GDPD2 (NM_017711) Human Tagged ORF Clone

CAT#: RG204415

GDPD2 (tGFP-tagged) - Human glycerophosphodiester phosphodiesterase domain containing 2 (GDPD2), transcript variant 2

Product Images

Circular map for RG204415

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: GDPD2
• Synonyms: GDE3; OBDPF
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG204415 representing NM_017711
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGAGTCCCCCGGCTGCTGCTCCGTCTGGGCCCGCTGCCTCCACTGCCTGTATAGCTGCCACTGGA
  GGAAATGCCCCAGAGAGAGGATGCAAACCAGCAAGTGCGACTGTATCTGGTTTGGCCTGCTCTTCCTCAC
  CTTCCTCCTTTCCCTGAGCTGGCTGTACATCGGGCTCGTCCTTCTCAATGACCTGCACAACTTCAATGAA
  TTCCTCTTCCGCCGCTGGGGACACTGGATGGACTGGTCCCTGGCATTCCTGCTGGTCATCTCTCTACTGG
  TCACATATGCATCCTTGCTATTGGTCCTGGCCCTGCTCCTGCGGCTTTGTAGACAGCCCCTGCATCTGCA
  CAGCCTCCACAAGGTGCTGCTGCTCCTCATTATGCTGCTTGTGGCGGCTGGCCTTGTGGGACTGGACATC
  CAATGGCAGCAGGAGTGGCATAGCTTGCGTGTGTCACTGCAGGCCACAGCCCCATTCCTTCATATTGGAG
  CAGCCGCTGGAATTGCCCTCCTGGCCTGGCCTGTGGCTGATACCTTCTACCGTATCCACCGAAGAGGTCC
  CAAGATTCTGCTACTGCTCCTATTTTTTGGAGTTGTCCTGGTCATCTACTTGGCCCCCCTATGCATCTCC
  TCACCCTGCATCATGGAACCCAGAGACTTACCACCCAAGCCTGGGCTGGTGGGACACCGAGGGGCCCCCA
  TGCTGGCTCCCGAGAACACCCTGATGTCCTTGCGGAAGACAGCTGAATGCGGAGCTACTGTGTTTGAGAC
  TGATGTGATGGTCAGCTCCGATGGGGTCCCCTTCCTCATGCATGATGAGCACCTCAGCAGGACCACGAAT
  GTAGCCTCTGTATTCCCAACCCGAATCACAGCCCACAGCAGTGACTTCTCCTGGACTGAACTGAAGAGAC
  TCAATGCTGGATCCTGGTTCCTAGAGAGGCGACCCTTCTGGGGGGCCAAACCGCTGGCAGGCCCTGATCA
  GAAAGAGGCTGAGAGTCAGACGGTACCAGCATTAGAAGAGCTATTGGAGGAAGCTGCAGCCCTCAACCTT
  TCCATCATGTTCGACTTGCGCCGACCCCCACAGAACCACACATACTATGACACTTTTGTGATCCAGACAT
  TGGAGACTGTGCTGAATGCAAGGGTGCCCCAAGCCATGGTCTTTTGGCTACCAGATGAAGATCGGGCTAA
  TGTCCAACGACGGGCACCTGGAATGCGCCAGATATATGGACGTCAGGGAGGCAACAGAACGGAGAGGCCC
  CAGTTTCTTAACCTCCCCTATCAAGATCTGCCACTATTGGATATCAAGGCATTGCATAAGGATAATGTCT
  CGGTGAACCTATTTGTAGTGAACAAGCCCTGGCTCTTCTCTCTGCTTTGGTGTGCAGGGGTGGATTCGGT
  CACCACCAACGACTGCCAGCTGCTGCAGCAGATGCGTTACCCTATCTGGCTTATTACCCCTCAAACCTAC
  CTAATCATATGGGTCATTACCAATTGTGTTTCCACCATGCTGCTTTTGTGGACCTTCCTCCTCCAAAGGA
  GATTTGTTAAGAAGAGAGGGAAAACTGGCTTAGAAACAGCAGTGCTGCTGACAAGGATCAACAATTTCAT
  GATGGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG204415 representing NM_017711
  Red=Cloning site Green=Tags(s)
  
  MAESPGCCSVWARCLHCLYSCHWRKCPRERMQTSKCDCIWFGLLFLTFLLSLSWLYIGLVLLNDLHNFNE
  FLFRRWGHWMDWSLAFLLVISLLVTYASLLLVLALLLRLCRQPLHLHSLHKVLLLLIMLLVAAGLVGLDI
  QWQQEWHSLRVSLQATAPFLHIGAAAGIALLAWPVADTFYRIHRRGPKILLLLLFFGVVLVIYLAPLCIS
  SPCIMEPRDLPPKPGLVGHRGAPMLAPENTLMSLRKTAECGATVFETDVMVSSDGVPFLMHDEHLSRTTN
  VASVFPTRITAHSSDFSWTELKRLNAGSWFLERRPFWGAKPLAGPDQKEAESQTVPALEELLEEAAALNL
  SIMFDLRRPPQNHTYYDTFVIQTLETVLNARVPQAMVFWLPDEDRANVQRRAPGMRQIYGRQGGNRTERP
  QFLNLPYQDLPLLDIKALHKDNVSVNLFVVNKPWLFSLLWCAGVDSVTTNDCQLLQQMRYPIWLITPQTY
  LIIWVITNCVSTMLLLWTFLLQRRFVKKRGKTGLETAVLLTRINNFMME
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017711
• ORF Size: 1617 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017711.2, NP_060181.2
• RefSeq Size: 2199 bp
• RefSeq ORF: 1620 bp
• Locus ID: 54857
• UniProt ID: Q9HCC8
• Cytogenetics: Xq13.1
• Protein Families: Transmembrane
• Gene Summary: This gene encodes a member of the glycerophosphodiester phosphodiesterase enzyme family. The encoded protein hydrolyzes glycerophosphoinositol to produce inositol 1-phosphate and glycerol. This protein may have a role in osteoblast differentiation and growth. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2010]