Myosin light chain 3 (MYL3) (NM_000258) Human Tagged ORF Clone

CAT#: RG203122

MYL3 (tGFP-tagged) - Human myosin, light chain 3, alkali; ventricular, skeletal, slow (MYL3)

Product Images

Circular map for RG203122

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Myosin light chain 3
• Synonyms: CMH8; MLC-lV/sb; MLC1SB; MLC1V; VLC1; VLCl
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG203122 representing NM_000258
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCCCAAAAAGCCAGAGCCCAAGAAGGATGATGCCAAGGCAGCCCCCAAGGCAGCTCCAGCTCCCG
  CACCTCCCCCTGAGCCTGAGCGCCCTAAGGAGGTCGAGTTTGATGCTTCCAAGATCAAGATTGAGTTCAC
  ACCTGAGCAGATTGAAGAGTTCAAGGAAGCCTTCATGCTGTTCGACCGCACACCCAAGTGTGAGATGAAG
  ATCACCTACGGGCAGTGTGGGGATGTCCTGCGGGCGCTGGGCCAGAACCCCACACAGGCAGAAGTGCTCC
  GTGTCCTGGGGAAGCCAAGACAGGAAGAGCTCAATACCAAGATGATGGACTTTGAAACTTTCCTGCCTAT
  GCTCCAGCACATTTCCAAGAACAAGGACACAGGCACCTATGAGGACTTCGTGGAGGGGCTGCGGGTCTTC
  GACAAGGAGGGCAATGGCACTGTCATGGGTGCTGAGCTTCGCCACGTGCTGGCCACGCTGGGTGAGAGGC
  TGACAGAAGACGAAGTGGAGAAGTTGATGGCTGGGCAAGAGGACTCCAATGGCTGCATCAACTATGAAGC
  ATTTGTGAAGCACATCATGTCCAGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG203122 representing NM_000258
  Red=Cloning site Green=Tags(s)
  
  MAPKKPEPKKDDAKAAPKAAPAPAPPPEPERPKEVEFDASKIKIEFTPEQIEEFKEAFMLFDRTPKCEMK
  ITYGQCGDVLRALGQNPTQAEVLRVLGKPRQEELNTKMMDFETFLPMLQHISKNKDTGTYEDFVEGLRVF
  DKEGNGTVMGAELRHVLATLGERLTEDEVEKLMAGQEDSNGCINYEAFVKHIMSS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000258
• ORF Size: 585 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000258.3
• RefSeq Size: 872 bp
• RefSeq ORF: 588 bp
• Locus ID: 4634
• UniProt ID: P08590
• Cytogenetics: 3p21.31
• Protein Families: Druggable Genome
• Protein Pathways: Cardiac muscle contraction, Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM)
• Gene Summary: MYL3 encodes myosin light chain 3, an alkali light chain also referred to in the literature as both the ventricular isoform and the slow skeletal muscle isoform. Mutations in MYL3 have been identified as a cause of mid-left ventricular chamber type hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008]