Myosin light chain 3 (MYL3) (NM_000258) Human Untagged Clone

CAT#: SC122554

MYL3 (untagged)-Human myosin, light chain 3, alkali, ventricular, skeletal, slow (MYL3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Myosin light chain 3
• Synonyms: CMH8; MLC-lV/sb; MLC1SB; MLC1V; VLC1; VLCl
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000258 edited
  GGGGACCCTGCTTTCTGCATTCTTCTCTCCACATCCCTCTCTGTACTTACAGCCCCCAAT
  GGCCCCCAAAAAGCCAGAGCCCAAGAAGGATGATGCCAAGGCAGCCCCCAAGGCAGCTCC
  AGCTCCCGCACCTCCCCCTGAGCCTGAGCGCCCTAAGGAGGTCGAGTTTGATGCTTCCAA
  GATCAAGATTGAGTTCACACCTGAGCAGATTGAAGAGTTCAAGGAAGCCTTCATGCTGTT
  CGACCGCACACCCAAGTGTGAGATGAAGATCACCTACGGGCAGTGTGGGGATGTCCTGCG
  GGCGCTGGGCCAGAACCCCACACAGGCAGAAGTGCTCCGTGTCCTGGGGAAGCCAAGACA
  GGAAGAGCTCAATACCAAGATGATGGACTTTGAAACTTTCCTGCCTATGCTCCAGCACAT
  TTCCAAGAACAAGGACACAGGCACCTATGAGGACTTCGTGGAGGGGCTGCGGGTCTTCGA
  CAAGGAGGGCAATGGCACTGTCATGGGTGCTGAGCTTCGCCACGTGCTGGCCACGCTGGG
  TGAGAGGCTGACAGAAGACGAAGTGGAGAAGTTGATGGCTGGGCAAGAGGACTCCAATGG
  CTGCATCAACTATGAAGCATTTGTGAAGCACATCATGTCCAGCTAAACCTCGTGCCCAGG
  AAGCCCAGGGAAGGCTGTGCAGGGACGTCTCATCTCCCATGTGTGATGCTGACACCAGCG
  GCCTGGAGTCGTGGGAAGGAGGGGAGCCTTACCAAGACTCCTGCAAAAACCCTTGGACCT
  CTCCACGTGGTTGCCGTCTCTGGCCCCTCTCAGGCTGTGCTTACCTGTGACAGCAGCTCT
  GTCCCCACCCCATGGGCCTCATGAATAAATGGCTTCTTGCCGGCAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000258 unedited
  NNGGACGCTTGGATTTGTATACGACTTATATAGGGCGGCCGCGATTCGGCCATTACGGCC
  GGGGACCCTGCTTTCTGCATTCTTCTCTCCACATCCCTCTCTGTACTTACAGCCCCCAAT
  GGCCCCCAAAAAGCCAGAGCCCAAGAAGGATGATGCCAAGGCAGCCCCCAAGGCAGCTCC
  AGCTCCCGCACCTCCCCCTGAGCCTGAGCGCCCTAAGGAGGTCGAGTTTGATGCTTCCAA
  GATCAAGATTGAGTTCACACCTGAGCAGATTGAAGAGTTCAAGGAAGCCTTCATGCTGTT
  CGACCGCACACCCAAGTGTGAGATGAAGATCACCTACGGGCAGTGTGGGGATGTCCTGCG
  GGCGCTGGGCCAGAACCCCACACAGGCAGAAGTGCTCCGTGTCCTGGGGAAGCCAAGACA
  GGAAGAGCTCAATACCAAGATGATGGACTTTGAAACTTTCCTGCCTATGCTCCAGCACAT
  TTCCAAGAACAAGGACACAGGCACCTATGAGGACTTCGTGGAGGGGCTGCGGGTCTTCGA
  CAAGGAGGGCAATGGCACTGTCATGGGTGCTGAGCTTCGCCACGTGCTGGCCACGCTGGG
  TGAGAGGCTGACAGAAGACGAAGTGGAGAAGTTGATGGCTGGGCAAGAGGACTCCAATGG
  CTGCATCAACTATGAAGCATTTGTGAAGCACATCATGTCCAGCTAAACCTCGTGCCCAGG
  AAGCCCATGGAATGCTGTGCATGGACGTCTCATCTCCCATGTGTGATGCTGACACCANNC
  GCCTGGAGTCGTGGGAAGGATGGGAGCCTTACCAAGACTCCTGGCAAAACCCTTGGNACC
  TCTCACGTGGNTTGCCGTCTCTGGCCCCTCTCAGGCTGTGCTTACCTGTGACAGCAGC
  
• Restriction Sites: Please inquire
• ACCN: NM_000258
• Insert Size: 959 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000258.1, NP_000249.1
• RefSeq Size: 872 bp
• RefSeq ORF: 588 bp
• Locus ID: 4634
• UniProt ID: P08590
• Cytogenetics: 3p21.31
• Protein Families: Druggable Genome
• Protein Pathways: Cardiac muscle contraction, Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM)
• Gene Summary: MYL3 encodes myosin light chain 3, an alkali light chain also referred to in the literature as both the ventricular isoform and the slow skeletal muscle isoform. Mutations in MYL3 have been identified as a cause of mid-left ventricular chamber type hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008]