DLK (DLK1) (NM_003836) Human Tagged ORF Clone
DLK1 (GFP-tagged) - Human delta-like 1 homolog (Drosophila) (DLK1)
|Type||Human Tagged ORF Clone|
|Synonyms||Delta1; DLK; DLK-1; FA1; pG2; Pref-1; PREF1; ZOG|
|E. coli Selection||Ampicillin (100 ug/mL)|
|Mammalian Cell Selection||Neomycin|
>RG202923 representing NM_003836
Red=Cloning site Blue=ORF Green=Tags(s)
ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG202923 representing NM_003836
Red=Cloning site Green=Tags(s)
TRTRPLE - GFP Tag - V
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||1149 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at email@example.com or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||1532 bp|
|RefSeq ORF||1152 bp|
|Domains||EGF_CA, EGF, EGF|
|Protein Families||Druggable Genome, ES Cell Differentiation/IPS, Transmembrane|
|Gene Summary||This gene encodes a transmembrane protein that contains multiple epidermal growth factor repeats that functions as a regulator of cell growth. The encoded protein is involved in the differentiation of several cell types including adipocytes. This gene is located in a region of chromosome 14 frequently showing unparental disomy, and is imprinted and expressed from the paternal allele. A single nucleotide variant in this gene is associated with child and adolescent obesity and shows polar overdominance, where heterozygotes carrying an active paternal allele express the phenotype, while mutant homozygotes are normal. [provided by RefSeq, Nov 2015]|
|cDNA Clone Resources|
|RC202923||DLK1 (Myc-DDK-tagged)-Human delta-like 1 homolog (Drosophila) (DLK1)||
|RC202923L1||Lenti ORF clone of Human delta-like 1 homolog (Drosophila) (DLK1), Myc-DDK-tagged||
|RC202923L2||Lenti ORF clone of Human delta-like 1 homolog (Drosophila) (DLK1), mGFP tagged||
|RC202923L3||Lenti ORF clone of Human delta-like 1 homolog (Drosophila) (DLK1), Myc-DDK-tagged||
|RC202923L4||Lenti ORF clone of Human delta-like 1 homolog (Drosophila) (DLK1), mGFP tagged||
|SC127962||DLK1 (untagged)-Human delta-like 1 homolog (Drosophila) (DLK1)||
|SC320741||DLK1 (untagged)-Human delta-like 1 homolog (Drosophila) (DLK1)||