eIF2B epsilon (EIF2B5) (NM_003907) Human Tagged ORF Clone

CAT#: RG202322

EIF2B5 (tGFP-tagged) - Human eukaryotic translation initiation factor 2B, subunit 5 epsilon, 82kDa (EIF2B5)

Product Images

Circular map for RG202322

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: eIF2B epsilon
• Synonyms: CACH; CLE; EIF-2B; EIF2Bepsilon; LVWM
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG202322 representing NM_003907
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCCCTGTAGTGGCGCCGCCTGGTGTGGTGGTTAGTCGGGCTAACAAGCGCAGCGGCGCGGGGC
  CGGGAGGCAGCGGTGGCGGGGGAGCCAGAGGGGCGGAGGAGGAACCGCCGCCGCCCCTACAAGCAGTTCT
  GGTGGCCGATAGCTTCGATCGCCGCTTCTTCCCCATCTCCAAGGACCAGCCTCGGGTCCTCTTGCCCCTG
  GCCAATGTGGCATTAATTGACTACACTCTGGAATTCCTGACTGCCACAGGTGTACAGGAAACATTTGTCT
  TTTGTTGCTGGAAAGCTGCTCAAATCAAAGAACATTTACTGAAGTCAAAGTGGTGCCGCCCTACATCTCT
  CAATGTGGTTCGAATAATTACATCAGAGCTCTATCGATCACTGGGAGATGTCCTCCGTGATGTTGATGCC
  AAGGCTTTGGTGCGCTCTGACTTTCTTCTGGTGTATGGGGATGTCATCTCAAACATCAATATCACCAGAG
  CCCTTGAGGAACACAGGTTGAGACGGAAGCTAGAAAAAAATGTTTCTGTGATGACGATGATCTTCAAGGA
  GTCATCCCCCAGCCACCCAACTCGTTGCCACGAAGACAATGTGGTAGTGGCTGTGGATAGTACCACAAAC
  AGGGTTCTCCATTTTCAGAAGACCCAGGGTCTCCGGCGTTTTGCATTTCCTCTGAGCCTGTTTCAGGGCA
  GTAGTGATGGAGTGGAGGTTCGATATGATTTACTGGATTGTCATATCAGCATCTGTTCTCCTCAGGTGGC
  ACAACTCTTTACAGACAACTTTGACTACCAAACTCGAGATGACTTTGTGCGAGGTCTCTTAGTGAATGAG
  GAGATCCTAGGGAACCAGATCCACATGCACGTAACAGCTAAGGAATATGGTGCCCGTGTCTCCAACCTAC
  ACATGTACTCAGCTGTCTGTGCTGACGTCATCCGCCGATGGGTCTACCCTCTCACCCCAGAGGCGAACTT
  CACTGACAGCACCACCCAGAGCTGCACTCATTCCCGGCACAACATCTACCGAGGGCCTGAGGTCAGCCTG
  GGCCATGGCAGCATCCTAGAGGAAAATGTGCTCCTGGGCTCTGGCACTGTCATTGGCAGCAATTGCTTTA
  TCACCAACAGTGTCATTGGCCCCGGCTGCCACATTGGTGATAACGTGGTGCTGGACCAGACCTACCTGTG
  GCAGGGTGTTCGAGTGGCGGCTGGAGCACAGATCCATCAGTCTCTGCTTTGTGACAATGCTGAGGTCAAG
  GAACGAGTGACACTGAAACCACGCTCTGTCCTCACTTCCCAGGTGGTCGTGGGCCCAAATATCACGCTGC
  CTGAGGGCTCGGTGATCTCTTTGCACCCTCCAGATGCAGAGGAAGATGAAGATGATGGCGAGTTCAGTGA
  TGATTCTGGGGCTGACCAAGAAAAGGACAAAGTGAAGATGAAAGGTTACAATCCAGCAGAAGTAGGAGCT
  GCTGGCAAGGGCTACCTCTGGAAAGCTGCAGGCATGAACATGGAGGAAGAGGAGGAACTGCAGCAGAATC
  TGTGGGGACTCAAGATCAACATGGAAGAAGAGAGTGAAAGTGAAAGTGAGCAAAGTATGGATTCTGAGGA
  GCCGGACAGCCGGGGAGGCTCCCCTCAGATGGATGACATCAAAGTGTTCCAGAATGAAGTTTTAGGAACA
  CTACAGCGGGGCAAAGAGGAGAACATTTCTTGTGACAATCTCGTCCTGGAAATCAACTCTCTCAAGTATG
  CCTATAACGTAAGTCTAAAGGAGGTGATGCAGGTACTGAGCCACGTGGTCCTGGAGTTCCCCCTGCAACA
  GATGGATTCCCCGCTTGACTCAAGCCGCTACTGTGCCCTGCTGCTTCCTCTGCTAAAGGCCTGGAGCCCT
  GTTTTTAGGAACTACATAAAGCGCGCAGCCGACCATTTGGAAGCGTTAGCAGCCATTGAGGACTTCTTCC
  TAGAGCATGAAGCTCTTGGTATTTCCATGGCCAAGGTACTGATGGCTTTCTACCAGCTGGAGATCCTGGC
  TGAGGAAACAATTCTGAGCTGGTTCAGCCAAAGAGATACAACTGACAAGGGCCAGCAGTTGCGCAAGAAT
  CAACAGCTGCAGAGGTTCATCCAGTGGCTAAAAGAGGCAGAAGAGGAGTCATCTGAAGATGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG202322 representing NM_003907
  Red=Cloning site Green=Tags(s)
  
  MAAPVVAPPGVVVSRANKRSGAGPGGSGGGGARGAEEEPPPPLQAVLVADSFDRRFFPISKDQPRVLLPL
  ANVALIDYTLEFLTATGVQETFVFCCWKAAQIKEHLLKSKWCRPTSLNVVRIITSELYRSLGDVLRDVDA
  KALVRSDFLLVYGDVISNINITRALEEHRLRRKLEKNVSVMTMIFKESSPSHPTRCHEDNVVVAVDSTTN
  RVLHFQKTQGLRRFAFPLSLFQGSSDGVEVRYDLLDCHISICSPQVAQLFTDNFDYQTRDDFVRGLLVNE
  EILGNQIHMHVTAKEYGARVSNLHMYSAVCADVIRRWVYPLTPEANFTDSTTQSCTHSRHNIYRGPEVSL
  GHGSILEENVLLGSGTVIGSNCFITNSVIGPGCHIGDNVVLDQTYLWQGVRVAAGAQIHQSLLCDNAEVK
  ERVTLKPRSVLTSQVVVGPNITLPEGSVISLHPPDAEEDEDDGEFSDDSGADQEKDKVKMKGYNPAEVGA
  AGKGYLWKAAGMNMEEEEELQQNLWGLKINMEEESESESEQSMDSEEPDSRGGSPQMDDIKVFQNEVLGT
  LQRGKEENISCDNLVLEINSLKYAYNVSLKEVMQVLSHVVLEFPLQQMDSPLDSSRYCALLLPLLKAWSP
  VFRNYIKRAADHLEALAAIEDFFLEHEALGISMAKVLMAFYQLEILAEETILSWFSQRDTTDKGQQLRKN
  QQLQRFIQWLKEAEEESSEDD
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003907
• ORF Size: 2163 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003907.1, NP_003898.1
• RefSeq Size: 2898 bp
• RefSeq ORF: 2166 bp
• Locus ID: 8893
• UniProt ID: Q13144
• Cytogenetics: 3q27.1
• Gene Summary: This gene encodes one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Mutations in this gene and the genes encoding other EIF2B subunits have been associated with leukoencephalopathy with vanishing white matter. [provided by RefSeq, Nov 2009]