eIF2B epsilon (EIF2B5) Rabbit Polyclonal Antibody

CAT#: TA375798

eIF2B epsilon (EIF2B5) Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using eIF2B epsilon (eIF2B epsilon (EIF2B5)) antibody (TA375798) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 10s.

• 

  Immunofluorescence analysis of L929 cells using eIF2B epsilon (eIF2B epsilon (EIF2B5)) Rabbit pAb (TA375798) at dilution of 1:100. Blue: DAPI for nuclear staining.

• 

  Immunoprecipitation analysis of 200ug extracts of A-549 cells using 3ug eIF2B epsilon (eIF2B epsilon (EIF2B5)) antibody ( TA375798). Western blot was performed from the immunoprecipitate using eIF2B epsilon (eIF2B epsilon (EIF2B5)) antibody ( TA375798) at a dilition of 1:1000.

Specifications

Product Data

• Applications: ICC/IF, IP, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:200
  IF,1:50 - 1:200
  IP,1:50 - 1:200
• Reactivities: Human, Mouse
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 442-721 of human eIF2B epsilon (eIF2B epsilon (EIF2B5)) (NP_003898.2).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 80kDa
• Gene Name: eukaryotic translation initiation factor 2B subunit epsilon
• Database Link:
  Entrez Gene 8893 Human
  UniProt ID Q13144
• Background: This gene encodes one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Mutations in this gene and the genes encoding other EIF2B subunits have been associated with leukoencephalopathy with vanishing white matter.
• Synonyms: CACH; CLE; EIF-2B; EIF2BE; EIF2Bepsilon; LVWM