Dishevelled 2 (DVL2) (NM_004422) Human Tagged ORF Clone

CAT#: RG202233

DVL2 (tGFP-tagged) - Human dishevelled, dsh homolog 2 (Drosophila) (DVL2)

Product Images

Circular map for RG202233

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Dishevelled 2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG202233 representing NM_004422
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGTAGCAGCACTGGGGGCGGTGGGGTTGGGGAGACGAAGGTGATTTACCACCTGGATGAGGAAG
  AGACTCCCTACCTGGTGAAGATCCCTGTCCCCGCCGAGCGCATCACCCTCGGCGATTTCAAGAGCGTCCT
  GCAGCGGCCCGCGGGCGCCAAGTACTTTTTCAAGTCTATGGATCAGGATTTCGGGGTGGTGAAGGAAGAA
  ATTTCAGATGACAACGCCCGCCTCCCCTGCTTCAACGGAAGGGTGGTATCCTGGCTGGTGTCCTCAGATA
  ATCCCCAACCCGAGATGGCCCCTCCAGTCCATGAGCCTCGGGCAGAACTGGCGCCTCCAGCCCCACCTTT
  ACCTCCTTTGCCACCCGAGAGGACCAGCGGCATTGGGGACTCAAGGCCTCCATCCTTCCACCCTAATGTG
  TCCAGCAGCCATGAGAATCTGGAGCCTGAGACAGAAACCGAGTCAGTAGTGTCACTGAGGCGGGAGCGGC
  CTCGCAGGAGAGACAGCAGTGAGCATGGCGCTGGGGGCCACAGGACTGGTGGCCCCTCAAGGCTGGAGCG
  CCACCTGGCCGGATACGAGAGCTCCTCTACCCTCATGACCAGCGAGCTGGAGAGTACCAGCCTGGGGGAC
  TCGGACGAGGAGGACACCATGAGCAGGTTCAGCAGCTCCACGGAGCAGAGCAGTGCCTCCCGCCTCCTTA
  AGCGCCACCGGCGGCGAAGGAAGCAGAGGCCACCCCGCCTGGAGAGGACGTCATCCTTCAGCAGCGTCAC
  AGATTCCACAATGTCTCTCAATATCATCACAGTCACGCTAAACATGGAGAAGTACAACTTCCTGGGTATC
  TCCATTGTTGGCCAGAGCAATGAGCGGGGAGACGGAGGCATCTACATTGGCTCCATCATGAAGGGTGGGG
  CTGTGGCGGCCGACGGGCGCATTGAGCCAGGGGACATGCTTTTGCAGGTGAATGACATGAACTTTGAGAA
  CATGAGCAACGATGACGCTGTGCGGGTGCTGAGGGACATTGTGCACAAGCCTGGCCCCATTGTGCTGACT
  GTGGCCAAGTGCTGGGATCCCTCTCCTCAGGCCTATTTCACTCTCCCCCGAAATGAGCCCATCCAGCCAA
  TTGACCCTGCTGCCTGGGTGTCCCATTCCGCGGCTCTGACTGGCACCTTCCCAGCCTATCCAGGTTCCTC
  CTCCATGAGCACCATTACATCTGGATCGTCTTTGCCTGATGGCTGTGAAGGCCGGGGTCTCTCCGTCCAT
  ACGGACATGGCATCGGTGACCAAGGCCATGGCAGCTCCAGAGTCTGGACTGGAAGTCCGGGACCGCATGT
  GGCTCAAGATCACCATCCCTAATGCCTTTCTGGGCTCGGATGTGGTTGACTGGCTCTACCATCACGTGGA
  GGGCTTTCCTGAGCGGCGGGAGGCCCGCAAGTATGCCAGCGGGCTGCTCAAAGCAGGCCTGATCCGACAC
  ACCGTCAACAAGATCACCTTCTCTGAGCAGTGCTATTACGTCTTCGGAGACCTCAGTGGTGGCTGTGAGA
  GCTACCTAGTCAACCTGTCTCTCAATGACAACGATGGCTCCAGTGGGGCTTCAGACCAGGATACCCTGGC
  TCCTCTGCCTGGGGCCACCCCCTGGCCCCTGCTGCCCACTTTCTCCTACCAATACCCTGCCCCACACCCC
  TACAGCCCGCAGCCTCCACCCTACCATGAGCTTTCATCTTACACCTATGGTGGGGGCAGTGCCAGCAGCC
  AGCATAGTGAGGGCAGCCGGAGCAGTGGGTCGACACGGAGTGATGGGGGGGCAGGGCGCACGGGGAGGCC
  CGAGGAGCGGGCCCCCGAGTCCAAGTCCGGCAGTGGCAGTGAGTCTGAGCCCTCCAGCCGAGGGGGCAGC
  CTTCGGCGGGGTGGGGAAGCAAGTGGGACTAGCGATGGGGGCCCTCCTCCATCCAGAGGCTCAACTGGGG
  GTGCCCCTAATCTCCGAGCCCACCCAGGGCTCCATCCCTATGGACCGCCCCCTGGCATGGCCCTCCCCTA
  CAACCCCATGATGGTGGTCATGATGCCCCCACCTCCACCTCCAGTCCCTCCAGCAGTGCAGCCTCCGGGG
  GCCCCTCCAGTCAGAGACCTGGGCTCTGTGCCCCCAGAACTGACAGCCAGCCGCCAAAGCTTCCACATGG
  CCATGGGCAATCCCAGCGAGTTCTTTGTGGATGTTATG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG202233 representing NM_004422
  Red=Cloning site Green=Tags(s)
  
  MAGSSTGGGGVGETKVIYHLDEEETPYLVKIPVPAERITLGDFKSVLQRPAGAKYFFKSMDQDFGVVKEE
  ISDDNARLPCFNGRVVSWLVSSDNPQPEMAPPVHEPRAELAPPAPPLPPLPPERTSGIGDSRPPSFHPNV
  SSSHENLEPETETESVVSLRRERPRRRDSSEHGAGGHRTGGPSRLERHLAGYESSSTLMTSELESTSLGD
  SDEEDTMSRFSSSTEQSSASRLLKRHRRRRKQRPPRLERTSSFSSVTDSTMSLNIITVTLNMEKYNFLGI
  SIVGQSNERGDGGIYIGSIMKGGAVAADGRIEPGDMLLQVNDMNFENMSNDDAVRVLRDIVHKPGPIVLT
  VAKCWDPSPQAYFTLPRNEPIQPIDPAAWVSHSAALTGTFPAYPGSSSMSTITSGSSLPDGCEGRGLSVH
  TDMASVTKAMAAPESGLEVRDRMWLKITIPNAFLGSDVVDWLYHHVEGFPERREARKYASGLLKAGLIRH
  TVNKITFSEQCYYVFGDLSGGCESYLVNLSLNDNDGSSGASDQDTLAPLPGATPWPLLPTFSYQYPAPHP
  YSPQPPPYHELSSYTYGGGSASSQHSEGSRSSGSTRSDGGAGRTGRPEERAPESKSGSGSESEPSSRGGS
  LRRGGEASGTSDGGPPPSRGSTGGAPNLRAHPGLHPYGPPPGMALPYNPMMVVMMPPPPPPVPPAVQPPG
  APPVRDLGSVPPELTASRQSFHMAMGNPSEFFVDVM
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004422
• ORF Size: 2208 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004422.3
• RefSeq Size: 3046 bp
• RefSeq ORF: 2211 bp
• Locus ID: 1856
• UniProt ID: O14641
• Cytogenetics: 17p13.1
• Domains: DEP, DAX, PDZ, Dishevelled
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS
• Protein Pathways: Basal cell carcinoma, Colorectal cancer, Melanogenesis, Notch signaling pathway, Pathways in cancer, Wnt signaling pathway
• Gene Summary: This gene encodes a member of the dishevelled (dsh) protein family. The vertebrate dsh proteins have approximately 40% amino acid sequence similarity with Drosophila dsh. This gene encodes a 90-kD protein that undergoes posttranslational phosphorylation to form a 95-kD cytoplasmic protein, which may play a role in the signal transduction pathway mediated by multiple Wnt proteins. The mechanisms of dishevelled function in Wnt signaling are likely to be conserved among metazoans. [provided by RefSeq, Jul 2008]