Dishevelled 2 (DVL2) (NM_004422) Human Untagged Clone

CAT#: SC128046

DVL2 (untagged)-Human dishevelled, dsh homolog 2 (Drosophila) (DVL2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Dishevelled 2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC128046 sequence for NM_004422 edited (data generated by NextGen Sequencing)
  ATGGCGGGTAGCAGCACTGGGGGCGGTGGGGTTGGGGAGACGAAGGTGATTTACCACCTG
  GATGAGGAAGAGACTCCCTACCTGGTGAAGATCCCTGTCCCCGCCGAGCGCATCACCCTC
  GGCGATTTCAAGAGCGTCCTGCAGCGGCCCGCGGGCGCCAAGTACTTTTTCAAGTCTATG
  GATCAGGATTTCGGGGTGGTGAAGGAAGAAATTTCAGATGACAACGCCCGCCTCCCCTGC
  TTCAACGGAAGGGTGGTATCCTGGCTGGTGTCCTCAGATAATCCCCAACCCGAGATGGCC
  CCTCCAGTCCATGAGCCTCGGGCAGAACTGGCGCCTCCAGCCCCACCTTTACCTCCTTTG
  CCACCCGAGAGGACCAGCGGCATTGGGGACTCAAGGCCTCCATCCTTCCACCCTAATGTG
  TCCAGCAGCCATGAGAATCTGGAGCCTGAGACAGAAACCGAGTCAGTAGTGTCACTGAGG
  CGGGAGCGGCCTCGCAGGAGAGACAGCAGTGAGCATGGCGCTGGGGGCCACAGGACTGGT
  GGCCCCTCAAGGCTGGAGCGCCACCTGGCCGGATACGAGAGCTCCTCTACCCTCATGACC
  AGCGAGCTGGAGAGTACCAGCCTGGGGGACTCGGACGAGGAGGACACCATGAGCAGGTTC
  AGCAGCTCCACGGAGCAGAGCAGTGCCTCCCGCCTCCTTAAGCGCCACCGGCGGCGAAGG
  AAGCAGAGGCCACCCCGCCTGGAGAGGACGTCATCCTTCAGCAGCGTCACAGATTCCACA
  ATGTCTCTCAATATCATCACAGTCACGCTAAACATGGAGAAGTACAACTTCCTGGGTATC
  TCCATTGTTGGCCAGAGCAATGAGCGGGGAGACGGAGGCATCTACATTGGCTCCATCATG
  AAGGGTGGGGCTGTGGCGGCCGACGGGCGCATTGAGCCAGGGGACATGCTTTTGCAGGTG
  AATGACATGAACTTTGAGAACATGAGCAACGATGACGCTGTGCGGGTGCTGAGGGACATT
  GTGCACAAGCCTGGCCCCATTGTGCTGACTGTGGCCAAGTGCTGGGATCCCTCTCCTCAG
  GCCTATTTCACTCTCCCCCGAAATGAGCCCATCCAGCCAATTGACCCTGCTGCCTGGGTG
  TCCCATTCCGCGGCTCTGACTGGCACCTTCCCAGCCTATCCAGGTTCCTCCTCCATGAGC
  ACCATTACATCTGGATCGTCTTTGCCTGATGGCTGTGAAGGCCGGGGTCTCTCCGTCCAT
  ACGGACATGGCATCGGTGACCAAGGCCATGGCAGCTCCAGAGTCTGGACTGGAAGTCCGG
  GACCGCATGTGGCTCAAGATCACCATCCCTAATGCCTTTCTGGGCTCGGATGTGGTTGAC
  TGGCTCTACCATCACGTGGAGGGCTTTCCTGAGCGGCGGGAGGCCCGCAAGTATGCCAGC
  GGGCTGCTCAAAGCAGGCCTGATCCGACACACCGTCAACAAGATCACCTTCTCTGAGCAG
  TGCTATTACGTCTTCGGAGACCTCAGTGGTGGCTGTGAGAGCTACCTAGTCAACCTGTCT
  CTCAATGACAACGATGGCTCCAGTGGGGCTTCAGACCAGGATACCCTGGCTCCTCTGCCT
  GGGGCCACCCCCTGGCCCCTGCTGCCCACTTTCTCCTACCAATACCCTGCCCCACACCCC
  TACAGCCCGCAGCCTCCACCCTACCATGAGCTTTCATCTTACACCTATGGTGGGGGCAGT
  GCCAGCAGCCAGCATAGTGAGGGCAGCCGGAGCAGTGGGTCGACACGGAGTGATGGGGGG
  GCAGGGCGCACGGGGAGGCCCGAGGAGCGGGCCCCCGAGTCCAAGTCCGGCAGTGGCAGT
  GAGTCTGAGCCCTCCAGCCGAGGGGGCAGCCTTCGGCGGGGTGGGGAAGCAAGTGGGACT
  AGCGATGGGGGCCCTCCTCCATCCAGAGGCTCAACTGGGGGTGCCCCTAATCTCCGAGCC
  CACCCAGGGCTCCATCCCTATGGACCGCCCCCTGGCATGGCCCTCCCCTACAACCCCATG
  ATGGTGGTCATGATGCCCCCACCTCCACCTCCAGTCCCTCCAGCAGTGCAGCCTCCGGGG
  GCCCCTCCAGTCAGAGACCTGGGCTCTGTGCCCCCAGAACTGACAGCCAGCCGCCAAAGC
  TTCCACATGGCCATGGGCAATCCCAGCGAGTTCTTTGTGGATGTTATGTAG
  
  Clone variation with respect to NM_004422.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004422 unedited
  GCCACAGGAGCTACACGAATACCACAGGGCGGCCGCGAAACGGCACGAGGGGGCGGCGGA
  GCCCGGGAGCGCGCGGGCGAGACACGGCGGGAAGCAGCACCGGGGGCGGCGGGGACGGGG
  AGACGAAGGCGATCTACCACCTGGATGAGGAAGAGACTCCCTACCTGGTGAAGACCCCTG
  TCCCCGCCGAGCGCATCACCCTCGGCGATTTCAAGAGCGTCCTGCAGCGGCCCGCGGGCG
  CCAAGTACTTTTTCAAGTCTATGGATCAGGATTTCGGGGTGGTGAAGGAAGAAATTTCAG
  ATGACAACGCCCGCCTCCCCTGCTTCAACGGAAGGGTGGTATCCTGGCTGGTGTCCTCAG
  ATAATCCCCAACCCGAGATGGCCCCTCCAGTCCATGAGCCTCGGGCAGAACTGGCGCCTC
  CAGCCCCACCTTTACCTCCTTTGCCACCCGAGAGGACCAGCGGCATTGGGGACTCAAGGC
  CTCCATCCTTCCACCCTAATGTGTCCAGCAGCCATGAGAATCTGGAGCCTGAGACAGAAA
  CCGAGTCAGTAGTGTCACTGAGGCGGGAGCGGCCTCGCAGGAGAGACAGCAGTGAGCATG
  GCGCTGGGGGCCACAGGACTGGTGGCCCCTCAAGGCTGGAGCGCCACCTGGCCGGATACG
  AGAGCTCCTCTACCCTCATGACCAGCGAGCTGGAGAGTACCAGCCTGGGGGACTCGGACG
  AGAGGACACCATGAGCAGTTCAGCAGCTCCACGGAGCAGAGCAGTGCCTCCCGCCTCCTT
  AAGCGCCACCGGCGGCGAAGAAGCAGAGGCCACCCCCGCCTGAGAGGACGTCATCCTTCA
  GCAGCGTCACAGATTCCACAATGTCTCTCATATCATCACAGTCACGCTAAACATGGAGAA
  GTACACTTCCTGGGTATCTCCATTGTTGGCCAGAGCAATGAGCGGGGGAGACGAAGCATC
  TACA
  
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_004422 unedited
  GGGGCCCCTTCCATCCATCCTCCTCATGCAGCCTCCGGGGCCCCTCCATCAGAGACCTGG
  GCTCTGTGCCCCCANAACTGACAGCCAGCCGCCAAAGCTTCCACATGGCCATGGGCAATC
  CCAGCGAGTTCTTTGTGGATGTTATGTAGCCCACTGTGGGGCCAGGCTGGGCCGGGCGCT
  CCTGGTGTGTGACTGGGTGTCCTGGCCGTCATGTGCTTGCTCTTACAGTGCCTGGGCTCA
  GCCTACCAGCTGCTGCCATACAGGAGATTGTGGCCACTGTGACTCTCACCAGCAGTGCCT
  GGTTCCTCCCCCTTCCCTCAGGGGTAGACAAGGGACCTTTGATTATTTTTAGCTTTGTTT
  TTTTATAAGCCTTTTTGGGGGTTAAAATAGAGTTTCTTACATTTTTGGGACTTTTTTAAT
  AGGCATTTCCTCTTTTATATGAAGAATTCCCATCCATTGGGCCCCTTCTAACCCCAGAAT
  GTGACCTCCTCCTCCAGTTACCCACAGCCCTGCCCTTTGCAGGGTTGGGGGTGGTCAGCG
  GTACCCCGGGGTTAGGCATCCTAGACAGCAGCCTGAGGAAGCTGGGAGATTTGGGCCATG
  TAGCTGCCTTTGTTACTCTATTTATTTTAGTCACTTGTATAAAACACCAAATAAAGCAAT
  AGAGGCAAACTCAAAANANAAAAANGACAAGAAGGAAGGTGCGGAGGGCAAACCTGCGCC
  ACACACAAAAAATCCAACACAAGAAAGACCCAAAGAAAANAAGAAGCGAGACAACCCCAC
  CTAAATAAAGAAAACTTCTTTTTTGTTTGGGGGCCGGGACAATTATTTTTTCCAAAAAAA
  ATCCGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004422
• Insert Size: 3050 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004422.2, NP_004413.1
• RefSeq Size: 3046 bp
• RefSeq ORF: 2211 bp
• Locus ID: 1856
• UniProt ID: O14641
• Cytogenetics: 17p13.1
• Domains: DEP, DAX, PDZ, Dishevelled
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS
• Protein Pathways: Basal cell carcinoma, Colorectal cancer, Melanogenesis, Notch signaling pathway, Pathways in cancer, Wnt signaling pathway
• Gene Summary: This gene encodes a member of the dishevelled (dsh) protein family. The vertebrate dsh proteins have approximately 40% amino acid sequence similarity with Drosophila dsh. This gene encodes a 90-kD protein that undergoes posttranslational phosphorylation to form a 95-kD cytoplasmic protein, which may play a role in the signal transduction pathway mediated by multiple Wnt proteins. The mechanisms of dishevelled function in Wnt signaling are likely to be conserved among metazoans. [provided by RefSeq, Jul 2008]