LAPTM4A (NM_014713) Human Tagged ORF Clone

CAT#: RG201290

LAPTM4A (tGFP-tagged) - Human lysosomal protein transmembrane 4 alpha (LAPTM4A)

Product Images

Circular map for RG201290

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: LAPTM4A
• Synonyms: HUMORF13; LAPTM4; MBNT; Mtrp
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG201290 representing NM_014713
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGTCCATGAGTTTCAAGCGGAACCGCAGTGACCGGTTCTACAGCACCCGGTGCTGCGGCTGTTGCC
  ATGTCCGCACCGGGACGATCATCCTGGGGACCTGGTACATGGTAGTAAACCTATTGATGGCAATTTTGCT
  GACTGTGGAAGTGACTCATCCAAACTCCATGCCAGCTGTCAACATTCAGTATGAAGTCATCGGTAATTAC
  TATTCTTCTGAGAGAATGGCTGATAATGCCTGTGTTCTTTTTGCCGTCTCTGTTCTTATGTTTATAATCA
  GTTCAATGCTGGTTTATGGATCAATTTCTTATCAAGTGGGTTGGCTGATTCCATTCTTCTGTTACCGACT
  TTTTGACTTCGTCCTCAGTTGCCTGGTTGCTATTAGTTCTCTCACCTATTTGCCAAGAATCAAAGAATAT
  CTGGATCAACTACCTGATTTTCCCTACAAAGATGACCTCCTGGCCTTGGACTCCAGCTGCCTCCTGTTCA
  TTGTTCTTGTGTTCTTTGCCTTATTCATCATTTTTAAGGCTTATCTAATTAACTGTGTTTGGAACTGCTA
  TAAATACATCAACAACCGAAACGTGCCGGAGATTGCTGTGTACCCTGCCTTTGAAGCACCTCCTCAGTAC
  GTTTTGCCAACCTATGAAATGGCCGTGAAAATGCCTGAAAAAGAACCACCACCTCCTTACTTACCTGCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG201290 representing NM_014713
  Red=Cloning site Green=Tags(s)
  
  MVSMSFKRNRSDRFYSTRCCGCCHVRTGTIILGTWYMVVNLLMAILLTVEVTHPNSMPAVNIQYEVIGNY
  YSSERMADNACVLFAVSVLMFIISSMLVYGSISYQVGWLIPFFCYRLFDFVLSCLVAISSLTYLPRIKEY
  LDQLPDFPYKDDLLALDSSCLLFIVLVFFALFIIFKAYLINCVWNCYKYINNRNVPEIAVYPAFEAPPQY
  VLPTYEMAVKMPEKEPPPPYLPA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014713
• ORF Size: 699 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014713.3, NP_055528.1
• RefSeq Size: 1419 bp
• RefSeq ORF: 702 bp
• Locus ID: 9741
• UniProt ID: Q15012
• Cytogenetics: 2p24.1
• Domains: Mtp
• Protein Families: Transmembrane
• Protein Pathways: Lysosome
• Gene Summary: This gene encodes a protein that has four predicted transmembrane domains. The function of this gene has not yet been determined; however, studies in the mouse homolog suggest a role in the transport of small molecules across endosomal and lysosomal membranes. [provided by RefSeq, Jul 2008]