LAPTM4A (NM_014713) Human Tagged ORF Clone

CAT#: RG201290

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  • TrueORF®

LAPTM4A (tGFP-tagged) - Human lysosomal protein transmembrane 4 alpha (LAPTM4A)



Reconstitution Protocol

USD 500.00

In Stock*

Size
    • 10 ug

Product images

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Symbol LAPTM4A
Synonyms HUMORF13; LAPTM4; MBNT; Mtrp
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG201290 representing NM_014713
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGTCCATGAGTTTCAAGCGGAACCGCAGTGACCGGTTCTACAGCACCCGGTGCTGCGGCTGTTGCC
ATGTCCGCACCGGGACGATCATCCTGGGGACCTGGTACATGGTAGTAAACCTATTGATGGCAATTTTGCT
GACTGTGGAAGTGACTCATCCAAACTCCATGCCAGCTGTCAACATTCAGTATGAAGTCATCGGTAATTAC
TATTCTTCTGAGAGAATGGCTGATAATGCCTGTGTTCTTTTTGCCGTCTCTGTTCTTATGTTTATAATCA
GTTCAATGCTGGTTTATGGATCAATTTCTTATCAAGTGGGTTGGCTGATTCCATTCTTCTGTTACCGACT
TTTTGACTTCGTCCTCAGTTGCCTGGTTGCTATTAGTTCTCTCACCTATTTGCCAAGAATCAAAGAATAT
CTGGATCAACTACCTGATTTTCCCTACAAAGATGACCTCCTGGCCTTGGACTCCAGCTGCCTCCTGTTCA
TTGTTCTTGTGTTCTTTGCCTTATTCATCATTTTTAAGGCTTATCTAATTAACTGTGTTTGGAACTGCTA
TAAATACATCAACAACCGAAACGTGCCGGAGATTGCTGTGTACCCTGCCTTTGAAGCACCTCCTCAGTAC
GTTTTGCCAACCTATGAAATGGCCGTGAAAATGCCTGAAAAAGAACCACCACCTCCTTACTTACCTGCC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG201290 representing NM_014713
Red=Cloning site Green=Tags(s)

MVSMSFKRNRSDRFYSTRCCGCCHVRTGTIILGTWYMVVNLLMAILLTVEVTHPNSMPAVNIQYEVIGNY
YSSERMADNACVLFAVSVLMFIISSMLVYGSISYQVGWLIPFFCYRLFDFVLSCLVAISSLTYLPRIKEY
LDQLPDFPYKDDLLALDSSCLLFIVLVFFALFIIFKAYLINCVWNCYKYINNRNVPEIAVYPAFEAPPQY
VLPTYEMAVKMPEKEPPPPYLPA

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_014713
ORF Size 699 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_014713.3, NP_055528.1
RefSeq Size 1419 bp
RefSeq ORF 702 bp
Locus ID 9741
UniProt ID Q15012
Cytogenetics 2p24.1
Domains Mtp
Protein Families Transmembrane
Protein Pathways Lysosome
Gene Summary This gene encodes a protein that has four predicted transmembrane domains. The function of this gene has not yet been determined; however, studies in the mouse homolog suggest a role in the transport of small molecules across endosomal and lysosomal membranes. [provided by RefSeq, Jul 2008]
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.