USP10 (NM_005153) Human Tagged ORF Clone

CAT#: RG200835

USP10 (tGFP-tagged) - Human ubiquitin specific peptidase 10 (USP10)

Product Images

Circular map for RG200835

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: USP10
• Synonyms: UBPO
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG200835 representing NM_005153
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCTCCACAGCCCGCAGTATATTTTTGGAGATTTTAGCCCTGATGAATTCAATCAATTCTTTGTGA
  CTCCTCGATCTTCAGTTGAGCTTCCTCCATACAGTGGAACAGTTCTGTGTGGCACACAGGCTGTGGATAA
  ACTACCTGATGGACAAGAATATCAGAGAATTGAGTTTGGTGTCGATGAAGTCATTGAACCCAGTGACACT
  TTGCCGAGAACCCCCAGCTACAGTATTTCAAGCACACTGAACCCTCAGGCCCCTGAATTTATTCTCGGTT
  GTACAGCTTCCAAAATAACCCCTGATGGTATCACTAAAGAAGCAAGCTATGGCTCCATCGACTGCCAGTA
  CCCAGGCTCTGCCCTCGCTTTGGATGGAAGTTCTAATGTGGAGGCGGAAGTTTTGGAAAATGATGGTGTC
  TCAGGTGGTCTTGGACAAAGGGAGCGTAAAAAGAAGAAAAAGCGGCCACCTGGATATTACAGCTATTTGA
  AAGATGGTGGCGATGATAGTATCTCCACAGAAGCCCTGGTCAATGGCCATGCCAATTCAGCAGTCCCGAA
  CAGTGTCAGTGCAGAGGATGCAGAATTTATGGGTGACATGCCTCCGCCACTTACGCCCAGGACTTGTAAC
  AGCCCCCAGAACTCCACAGACTCTGTCAGTGACATTGTGCCTGACAGTCCTTTCCCCGGAGCACTCGGCA
  GTGACACCAGGACTGCAGGGCAGCCAGAGGGGGGCCCCGGGGCTGATTTTGGTCAGTCCTGCTTCCCTGC
  AGAGGCTGGCAGAGACACCCTGTCAAGGACAGCTGGGGCTCAGCCCTGCGTTGGTACCGATACTACTGAA
  AACCTTGGAGTTGCTAATGGACAAATACTTGAATCCTCGGGTGAGGGCACAGCTACCAACGGGGTGGAGT
  TGCACACCACGGAAAGCATAGACTTGGACCCAACCAAACCCGAGAGTGCATCACCTCCTGCTGACGGCAC
  GGGCTCTGCATCAGGCACCCTTCCTGTCAGCCAGCCCAAGTCCTGGGCCAGCCTCTTTCATGATTCTAAG
  CCCTCTTCCTCCTCGCCGGTGGCCTATGTGGAAACTAAGTATTCCCCTCCCGCCATATCTCCCCTGGTTT
  CTGAAAAGCAGGTTGAAGTCAAAGAAGGGCTTGTTCCGGTTTCAGAGGATCCTGTAGCCATAAAGATTGC
  AGAGTTGCTGGAGAATGTAACCCTAATCCATAAACCAGTGTCGTTGCAACCCCGTGGGCTGATCAATAAA
  GGGAACTGGTGCTACATTAATGCTACACTGCAGGCATTGGTTGCTTGCCCGCCGATGTACCACCTGATGA
  AGTTCATTCCTCTGTATTCCAAAGTGCAAAGGCCTTGTACGTCAACACCCATGATAGACAGCTTTGTTCG
  GCTAATGAATGAGTTCACTAATATGCCAGTACCTCCAAAACCCCGACAAGCTCTTGGAGATAAAATCGTG
  AGGGATATTCGCCCTGGAGCTGCCTTTGAGCCCACATATATTTACAGACTCCTGACAGTTAACAAGTCAA
  GCCTGTCTGAAAAGGGTCGACAAGAAGATGCTGAGGAATACTTAGGCTTCATTCTAAATGGACTTCATGA
  GGAAATGTTGAACCTAAAGAAGCTTCTCTCACCAAGTAATGAAAAACTTACGATTTCCAACGGCCCCAAA
  AACCACTCGGTCAATGAAGAAGAGCAGGAAGAACAAGGTGAAGGAAGCGAGGATGAATGGGAACAAGTGG
  GCCCCCGGAACAAGACTTCCGTCACCCGCCAGGCGGATTTTGTTCAGACTCCAATCACCGGCATTTTTGG
  TGGACACATCAGGTCTGTGGTTTACCAGCAGAGTTCAAAAGAATCTGCCACTTTGCAGCCATTTTTCACG
  TTGCAGTTGGATATCCAGTCAGACAAGATACGCACAGTCCAGGATGCACTGGAGAGCTTGGTGGCAAGAG
  AATCTGTCCAAGGTTATACCACAAAAACCAAACAAGAGGTTGAGATAAGTCGAAGAGTGACTCTGGAAAA
  ACTCCCTCCTGTCCTCGTGCTGCACCTGAAACGATTCGTTTATGAGAAGACTGGTGGGTGCCAGAAGCTT
  ATCAAAAATATTGAATATCCTGTGGACTTGGAAATTAGTAAAGAACTGCTTTCTCCAGGGGTTAAAAATA
  AGAATTTTAAATGCCACCGAACCTATCGGCTCTTTGCAGTGGTCTACCATCACGGCAACAGTGCGACGGG
  CGGCCATTACACTACAGACGTCTTCCAGATCGGTCTGAATGGCTGGCTGCGCATCGATGACCAGACAGTC
  AAGGTGATCAACCAGTACCAGGTGGTGAAACCAACTGCTGAACGCACAGCCTACCTCCTGTATTACCGCC
  GAGTGGACCTGCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG200835 representing NM_005153
  Red=Cloning site Green=Tags(s)
  
  MALHSPQYIFGDFSPDEFNQFFVTPRSSVELPPYSGTVLCGTQAVDKLPDGQEYQRIEFGVDEVIEPSDT
  LPRTPSYSISSTLNPQAPEFILGCTASKITPDGITKEASYGSIDCQYPGSALALDGSSNVEAEVLENDGV
  SGGLGQRERKKKKKRPPGYYSYLKDGGDDSISTEALVNGHANSAVPNSVSAEDAEFMGDMPPPLTPRTCN
  SPQNSTDSVSDIVPDSPFPGALGSDTRTAGQPEGGPGADFGQSCFPAEAGRDTLSRTAGAQPCVGTDTTE
  NLGVANGQILESSGEGTATNGVELHTTESIDLDPTKPESASPPADGTGSASGTLPVSQPKSWASLFHDSK
  PSSSSPVAYVETKYSPPAISPLVSEKQVEVKEGLVPVSEDPVAIKIAELLENVTLIHKPVSLQPRGLINK
  GNWCYINATLQALVACPPMYHLMKFIPLYSKVQRPCTSTPMIDSFVRLMNEFTNMPVPPKPRQALGDKIV
  RDIRPGAAFEPTYIYRLLTVNKSSLSEKGRQEDAEEYLGFILNGLHEEMLNLKKLLSPSNEKLTISNGPK
  NHSVNEEEQEEQGEGSEDEWEQVGPRNKTSVTRQADFVQTPITGIFGGHIRSVVYQQSSKESATLQPFFT
  LQLDIQSDKIRTVQDALESLVARESVQGYTTKTKQEVEISRRVTLEKLPPVLVLHLKRFVYEKTGGCQKL
  IKNIEYPVDLEISKELLSPGVKNKNFKCHRTYRLFAVVYHHGNSATGGHYTTDVFQIGLNGWLRIDDQTV
  KVINQYQVVKPTAERTAYLLYYRRVDLL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005153
• ORF Size: 2394 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005153.1, NP_005144.1
• RefSeq Size: 3399 bp
• RefSeq ORF: 2397 bp
• Locus ID: 9100
• UniProt ID: Q14694
• Cytogenetics: 16q24.1
• Domains: UCH
• Protein Families: Druggable Genome, Protease
• Gene Summary: Ubiquitin is a highly conserved protein that is covalently linked to other proteins to regulate their function and degradation. This gene encodes a member of the ubiquitin-specific protease family of cysteine proteases. The enzyme specifically cleaves ubiquitin from ubiquitin-conjugated protein substrates. The protein is found in the nucleus and cytoplasm. It functions as a co-factor of the DNA-bound androgen receptor complex, and is inhibited by a protein in the Ras-GTPase pathway. The human genome contains several pseudogenes similar to this gene. Several transcript variants, some protein-coding and others not protein-coding, have been found for this gene. [provided by RefSeq, Jan 2013]