EIF3B (NM_001037283) Human Tagged ORF Clone

CAT#: RG200612

EIF3B (tGFP-tagged) - Human eukaryotic translation initiation factor 3, subunit B (EIF3B), transcript variant 2

Product Images

Circular map for RG200612

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: EIF3B
• Synonyms: EIF3-ETA; EIF3-P110; EIF3-P116; EIF3S9; PRT1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG200612 representing NM_001037283
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGGACGCGGAGAACGTGGCGGTGCCCGAGGCGGCCGAGGAGCGCGCCGAGCCCGGCCAGCAGCAGC
  CGGCCGCCGAGCCGCCGCCAGCCGAGGGGCTGCTGCGGCCCGCGGGGCCCGGCGCTCCGGAGGCCGCGGG
  GACCGAGGCCTCCAGTGAGGAGGTGGGGATCGCGGAGGCCGGGCCGGAGCCCGAGGTGAGGACCGAGCCG
  GCGGCCGAGGCAGAGGCGGCCTCCGGCCCGTCCGAGTCGCCCTCGCCGCCGGCCGCCGAGGAGCTGCCCG
  GGTCGCATGCTGAGCCCCCTGTCCCGGCACAGGGCGAGGCCCCAGGAGAGCAGGCTCGGGACGAGCGCTC
  CGACAGCCGGGCCCAGGCGGTGTCCGAGGACGCGGGAGGAAACGAGGGCAGAGCGGCCGAGGCCGAACCC
  CGGGCGCTGGAGAATGGCGACGCGGACGAGCCCTCCTTCAGCGACCCCGAGGACTTCGTGGACGACGTGA
  GCGAGGAAGAATTACTGGGAGATGTACTCAAAGATCGGCCCCAGGAAGCAGATGGAATCGATTCGGTGAT
  TGTAGTGGACAATGTCCCTCAGGTGGGACCCGACCGACTTGAGAAACTCAAAAATGTCATCCACAAGATC
  TTTTCCAAGTTTGGGAAAATCACAAATGATTTTTATCCTGAAGAGGATGGGAAGACAAAAGGGTATATTT
  TCCTGGAGTACGCGTCCCCTGCCCACGCTGTGGATGCTGTGAAGAACGCCGACGGCTACAAGCTTGACAA
  GCAGCACACATTCCGGGTCAACCTCTTTACGGATTTTGACAAGTATATGACGATCAGTGACGAGTGGGAT
  ATTCCAGAGAAACAGCCTTTCAAAGACCTGGGGAACTTACGTTACTGGCTTGAAGAGGCAGAATGCAGAG
  ATCAGTACAGTGTGATTTTTGAGAGTGGAGACCGCACTTCCATATTCTGGAATGACGTAAAAGACCCTGT
  CTCAATTGAAGAAAGAGCGAGATGGACAGAGACGTATGTGCGTTGGTCTCCTAAGGGCACCTACCTGGCT
  ACCTTTCATCAAAGAGGCATTGCTCTATGGGGGGGAGAGAAATTCAAGCAAATTCAGAGATTCAGCCACC
  AAGGGGTTCAGCTTATTGACTTCTCACCTTGTGAAAGGTACCTGGTGACCTTTAGCCCCCTGATGGACAC
  GCAGGATGACCCTCAGGCCATAATCATCTGGGACATCCTTACGGGGCACAAGAAGAGGGGTTTTCACTGT
  GAGAGCTCAGCCCATTGGCCTATTTTTAAGTGGAGCCATGATGGCAAATTCTTTGCCAGAATGACCCTGG
  ATACGCTTAGCATCTATGAAACTCCTTCTATGGGTCTTTTGGACAAGAAGAGTTTGAAGATCTCTGGGAT
  AAAAGACTTTTCTTGGTCTCCTGGTGGTAACATAATCGCCTTCTGGGTGCCTGAAGACAAAGATATTCCA
  GCCAGGGTAACCCTGATGCAGCTCCCTACCAGGCAAGAGATCCGAGTGAGGAACCTGTTCAATGTGGTGG
  ACTGCAAGCTCCATTGGCAGAAGAACGGAGACTACTTGTGTGTGAAAGTAGATAGGACTCCGAAAGGCAC
  CCAGGGTGTTGTCACAAATTTTGAAATTTTCCGAATGAGGGAGAAACAGGTACCTGTGGATGTGGTCGAG
  ATGAAAGAAACCATCATAGCCTTTGCCTGGGAACCAAATGGAAGTAAGTTTGCTGTGCTGCACGGAGAGG
  CTCCGCGGATATCTGTGTCTTTCTACCACGTCAAAAACAACGGGAAGATTGAACTCATCAAGATGTTCGA
  CAAGCAGCAGGCGAACACCATCTTCTGGAGCCCCCAAGGACAGTTCGTGGTGTTGGCGGGCCTGAGGAGT
  ATGAACGGTGCCTTAGCGTTTGTGGACACTTCGGACTGCACGGTCATGAACATCGCAGAGCACTACATGG
  CTTCCGACGTCGAATGGGATCCTACTGGGCGCTACGTCGTCACCTCTGTGTCCTGGTGGAGCCATAAGGT
  GGACAACGCGTACTGGCTGTGGACTTTCCAGGGACGCCTCCTGCAGAAGAACAACAAGGACCGCTTCTGC
  CAGCTGCTGTGGCGGCCCCGGCCTCCCACACTCCTGAGCCAGGAACAGATCAAGCAAATTAAAAAGGATC
  TGAAGAAATACTCTAAGATCTTTGAACAGAAGGATCGTTTGAGTCAGTCCAAAGCCTCAAAGGAATTGGT
  GGAGAGAAGGCGCACCATGATGGAAGATTTCCGGAAGTACCGGAAAATGGCCCAGGAGCTCTATATGGAG
  CAGAAAAACGAGCGCCTGGAGTTGCGAGGAGGGGTGGACACTGACGAGCTGGACAGCAACGTGGACGACT
  GGGAAGAGGAGACCATTGAGTTCTTCGTCACTGAAGAAATCATTCCCCTCGGGAATCAGGAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG200612 representing NM_001037283
  Red=Cloning site Green=Tags(s)
  
  MQDAENVAVPEAAEERAEPGQQQPAAEPPPAEGLLRPAGPGAPEAAGTEASSEEVGIAEAGPEPEVRTEP
  AAEAEAASGPSESPSPPAAEELPGSHAEPPVPAQGEAPGEQARDERSDSRAQAVSEDAGGNEGRAAEAEP
  RALENGDADEPSFSDPEDFVDDVSEEELLGDVLKDRPQEADGIDSVIVVDNVPQVGPDRLEKLKNVIHKI
  FSKFGKITNDFYPEEDGKTKGYIFLEYASPAHAVDAVKNADGYKLDKQHTFRVNLFTDFDKYMTISDEWD
  IPEKQPFKDLGNLRYWLEEAECRDQYSVIFESGDRTSIFWNDVKDPVSIEERARWTETYVRWSPKGTYLA
  TFHQRGIALWGGEKFKQIQRFSHQGVQLIDFSPCERYLVTFSPLMDTQDDPQAIIIWDILTGHKKRGFHC
  ESSAHWPIFKWSHDGKFFARMTLDTLSIYETPSMGLLDKKSLKISGIKDFSWSPGGNIIAFWVPEDKDIP
  ARVTLMQLPTRQEIRVRNLFNVVDCKLHWQKNGDYLCVKVDRTPKGTQGVVTNFEIFRMREKQVPVDVVE
  MKETIIAFAWEPNGSKFAVLHGEAPRISVSFYHVKNNGKIELIKMFDKQQANTIFWSPQGQFVVLAGLRS
  MNGALAFVDTSDCTVMNIAEHYMASDVEWDPTGRYVVTSVSWWSHKVDNAYWLWTFQGRLLQKNNKDRFC
  QLLWRPRPPTLLSQEQIKQIKKDLKKYSKIFEQKDRLSQSKASKELVERRRTMMEDFRKYRKMAQELYME
  QKNERLELRGGVDTDELDSNVDDWEEETIEFFVTEEIIPLGNQE
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001037283
• ORF Size: 2442 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001037283.1, NP_001032360.1
• RefSeq Size: 3084 bp
• RefSeq ORF: 2445 bp
• Locus ID: 8662
• UniProt ID: P55884
• Cytogenetics: 7p22.3
• Gene Summary: RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:9388245, PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:9388245, PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773).[UniProtKB/Swiss-Prot Function]