PIK3C2G (NM_001288772) Human Tagged ORF Clone

CAT#: RC240163

PIK3C2G (myc-DDK-tagged) - Human phosphatidylinositol-4-phosphate 3-kinase, catalytic subunit type 2 gamma (PIK3C2G), transcript variant 1

Product Images

Circular map for RC240163

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PIK3C2G
• Synonyms: PI3K-C2-gamma; PI3K-C2GAMMA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC240163 representing NM_001288772
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCATATTCTTGGCAAACGGATCCAAATCCTAATGAATCACACGAAAAGCAGTATGAACACCAAGAAT
  TTCTCTTTGTAAATCAACCCCATTCTTCTAGCCAAGTCAGTCTGGGTTTTGATCAGATAGTAGATGAGAT
  CAGTGGCAAAATTCCACACTACGAGAGTGAAATTGATGAAAACACCTTTTTTGTGCCCACTGCACCAAAA
  TGGGACTCAACAGGGCATTCATTAAATGAAGCACACCAAATATCCTTGAATGAATTCACTTCTAAAAGCC
  GTGAACTCTCCTGGCATCAAGTTAGCAAAGCACCAGCAATTGGTTTTAGTCCTTCTGTGTTACCAAAACC
  TCAAAATACGAATAAAGAATGCTCCTGGGGAAGCCCCATAGGAAAACATCATGGTGCTGATGATTCCAGA
  TTCAGTATTTTAGCTCCATCATTCACAAGTTTGGATAAAATTAATCTAGAGAAAGAATTAGAAAATGAAA
  ATCATAACTACCATATAGGATTTGAAAGTAGCATTCCTCCAACAAATTCATCCTTCTCAAGTGACTTCAT
  GCCGAAAGAAGAGAATAAAAGGAGTGGACATGTGAACATTGTGGAACCATCTTTGATGCTTTTGAAAGGC
  TCTCTTCAACCCGGAATGTGGGAAAGTACATGGCAGAAGAATATAGAGTCAATAGGTTGTTCCATTCAGC
  TAGTGGAAGTACCTCAAAGCAGCAATACGAGTCTGGCCTCTTTTTGCAACAAAGTAAAAAAAATCAGAGA
  AAGATATCATGCAGCTGATGTTAATTTCAATTCTGGGAAGATCTGGAGCACTACTACAGCATTTCCGTAT
  CAGCTCTTTTCTAAGACCAAGTTTAATATACATATTTTTATTGATAACTCAACACAACCTCTTCATTTTA
  TGCCATGTGCTAATTATCTTGTCAAAGATCTAATTGCAGAAATTCTGCATTTTTGCACAAATGACCAGCT
  ACTCCCCAAAGATCATATTCTAAGTGTATGTGGCTCTGAAGAATTTTTACAAAACGACCACTGTTTGGGG
  AGCCACAAAATGTTTCAAAAAGATAAATCTGTTATTCAGCTCCACCTGCAGAAAAGTAGGGAAGCTCCAG
  GAAAGCTATCTCGAAAGCATGAAGAGGACCACAGTCAGTTTTATCTGAATCAACTTCTAGAATTTATGCA
  TATTTGGAAAGTATCCAGACAATGTCTCTTAACACTCATCAGAAAATATGACTTCCACCTGAAATACCTA
  TTGAAAACCCAGGAAAACGTGTATAATATTATTGAAGAAGTTAAAAAAATATGCAGTGTTCTAGGGTGTG
  TGGAAACCAAACAAATTACAGATGCAGTAAATGAACTAAGTCTAATTCTTCAGAGAAAAGGAGAGAATTT
  TTATCAAAGTTCAGAGACTTCAGCAAAAGGCTTGATAGAGAAGGTAACAACTGAACTATCCACATCCATC
  TACCAGCTAATCAATGTCTACTGTAACAGCTTTTATGCAGATTTTCAGCCTGTAAATGTACCTAGATGCA
  CTTCCTATCTAAATCCCGGGCTTCCTTCCCACCTCAGCTTCACAGTGTATGCAGCACACAACATTCCAGA
  AACCTGGGTGCACAGCTACAAAGCGTTTTCTTTTACCTGTTGGCTTACATATGCTGGAAAGAAGCTGTGC
  CAAGTGAGAAACTACAGAAATATTCCAGACAAGAAATTATTTTTTTTCTTGGTCAACTGGAATGAAACGA
  TCAATTTTCCCCTTGAAATAAAGTCACTTCCAAGGGAATCCATGCTCACTGTAAAACTGTTTGGGATTGC
  CTGTGCAACCAACAATGCAAATTTACTGGCGTGGACTTGTCTTCCACTGTTTCCAAAAGAAAAATCCATT
  CTCGGGTCTATGCTGTTCAGCATGACATTACAGAGTGAGCCTCCCGTAGAAATGATAACTCCAGGAGTGT
  GGGATGTAAGTCAGCCATCCCCGGTGACCCTGCAGATTGATTTTCCAGCTACTGGGTGGGAGTATATGAA
  ACCTGATTCTGAAGAGAATAGAAGTAATCTTGAAGAGCCACTAAAGGAGTGTATAAAACATATTGCCAGA
  CTTTCACAGAAACAGACTCCCCTACTACTCTCTGAAGAAAAGAAAAGATATTTATGGTTTTATCGCTTCT
  ACTGCAATAATGAAAACTGCTCCCTTCCTTTAGTCCTGGGTAGTGCCCCTGGATGGGATGAAAGGACTGT
  TTCAGAAATGCATACCATTTTGAGAAGATGGACATTTTCTCAACCTTTAGAGGCTCTTGGGCTTTTGACT
  TCCAGTTTTCCAGATCAAGAAATTCGTAAAGTGGCAGTTCAACAATTAGACAACCTCTTGAATGATGAAC
  TACTGGAATATCTCCCACAGCTAGTTCAGGCTGTCAAGTTTGAATGGAACCTTGAGAGTCCTTTAGTGCA
  ACTTCTACTCCACCGCTCCTTGCAGAGCATCCAGGTTGCCCATCGTCTTTACTGGCTGCTAAAAAATGCA
  GAAAATGAAGCTTATTTTAAAAGCTGGTATCAGAAGCTACTAGCTGCTCTCCAATTCTGTGCAGGTAAAG
  CCTTGAATGATGAGTTTTCCAAGGAGCAGAAACTTATCAAAATTCTGGGAGATATTGGGGAAAGAGTCAA
  GTCTGCCAGTGACCATCAAAGACAGGAGGTACTGAAGAAAGAAATTGGCAGACTAGAAGAGTTCTTTCAA
  GATGTAAATACTTGTCATCTTCCTCTGAACCCTGCCCTATGTATAAAAGGGATTGATCACGATGCATGTT
  CATATTTTACATCTAATGCTTTGCCATTGAAGATTACTTTCATCAATGCTAATCCGATGGGCAAAAACAT
  CAGCATTATTTTTAAGGCTGGAGATGATCTTCGTCAGGATATGCTTGTTCTGCAGCTTATTCAAGTGATG
  GACAATATTTGGCTGCAGGAAGGCTTGGATATGCAAATGATCATTTATAGATGTCTATCCACAGGAAAAG
  ACCAAGGATTGGTGCAGATGGTACCTGATGCTGTGACCCTAGCAAAGATTCATCGCCATTCTGGACTGAT
  AGGACCATTGAAAGAAAATACAATTAAAAAGTGGTTCAGTCAGCACAACCACTTAAAGGCAGATTATGAA
  AAGGCCTTGAGGAACTTTTTCTACTCCTGTGCTGGCTGGTGTGTGGTAACATTCATCCTGGGAGTATGTG
  ACCGTCACAATGATAATATCATGCTGACAAAGTCGGGCCACATGTTTCATATTGACTTTGGAAAATTCTT
  AGGTCATGCACAAACATTTGGAGGGATAAAAAGGGACCGAGCTCCTTTCATTTTTACTTCAGAGATGGAA
  TACTTTATTACAGAGGGTGGGAAAAACCCACAGCATTTTCAAGATTTTGTGGAACTTTGCTGTCGTGCTT
  ATAATATTATCAGAAAGCACAGCCAACTGCTCTTGAACCTGCTGGAAATGATGCTGTATGCAGGACTGCC
  TGAGCTAAGTGGAATTCAAGACCTGAAATATGTGTATAATAATCTTCGTCCACAAGACACAGACCTGGAA
  GCAACAAGTCATTTTACCAAGAAAATAAAGGAAAGTCTGGAGTGTTTCCCTGTTAAATTGAATAACTTGA
  TCCACACACTTGCACAAATGTCAGCCATAAGCCCTGCCAAATCTACTTCACAGACTTTTCCTCAGGAATC
  CTGTTTGCTGAGTACAACTAGGTCGATTGAAAGAGCAACAATTTTAGGGTTCAGCAAGAAATCCAGTAAT
  CTGTATCTGATCCAGGTGACACACAGCAACAACGAAACAAGCCTGACAGAAAAATCATTTGAGCAGTTTT
  CAAAACTTCACAGCCAACTTCAGAAGCAGTTTGCATCACTGACTCTCCCAGAGTTTCCTCATTGGTGGCA
  CCTACCTTTTACAAATTCAGATCACAGAAGATTCAGAGATCTAAATCATTACATGGAACAGATATTAAAT
  GTATCACATGAAGTTACAAACAGTGATTGTGTACTTAGCTTTTTCCTCTCTGAGGCTGTGCAACAAACAG
  TTGAAGAATCATCACCTGTGTACCTAGGTGAGAAGTTTCCAGACAAGAAGCCTAAGGTGCAGTTAGTCAT
  ATCCTACGAGGATGTGAAGCTGACCATACTAGTGAAACACATGAAAAACATTCATCTCCCAGATGGCTCT
  GCGCCCAGTGCACATGTTGAATTTTATCTTTTACCATATCCCAGTGAAGTTCGTAGGAGGAAAACAAAAT
  CTGTTCCAAAATGTACGGACCCCACTTACAATGAAATTGTAGTATATGATGAAGTCACAGAGCTCCAAGG
  ACATGTCTTAATGCTTATTGTGAAGAGTAAAACTGTATTTGTGGGAGCAATTAACATCCGACTCTGTAGT
  GTCCCACTCGATAAAGAAAAATGGTATCCATTAGGAAACAGTATAATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC240163 representing NM_001288772
  Red=Cloning site Green=Tags(s)
  
  MAYSWQTDPNPNESHEKQYEHQEFLFVNQPHSSSQVSLGFDQIVDEISGKIPHYESEIDENTFFVPTAPK
  WDSTGHSLNEAHQISLNEFTSKSRELSWHQVSKAPAIGFSPSVLPKPQNTNKECSWGSPIGKHHGADDSR
  FSILAPSFTSLDKINLEKELENENHNYHIGFESSIPPTNSSFSSDFMPKEENKRSGHVNIVEPSLMLLKG
  SLQPGMWESTWQKNIESIGCSIQLVEVPQSSNTSLASFCNKVKKIRERYHAADVNFNSGKIWSTTTAFPY
  QLFSKTKFNIHIFIDNSTQPLHFMPCANYLVKDLIAEILHFCTNDQLLPKDHILSVCGSEEFLQNDHCLG
  SHKMFQKDKSVIQLHLQKSREAPGKLSRKHEEDHSQFYLNQLLEFMHIWKVSRQCLLTLIRKYDFHLKYL
  LKTQENVYNIIEEVKKICSVLGCVETKQITDAVNELSLILQRKGENFYQSSETSAKGLIEKVTTELSTSI
  YQLINVYCNSFYADFQPVNVPRCTSYLNPGLPSHLSFTVYAAHNIPETWVHSYKAFSFTCWLTYAGKKLC
  QVRNYRNIPDKKLFFFLVNWNETINFPLEIKSLPRESMLTVKLFGIACATNNANLLAWTCLPLFPKEKSI
  LGSMLFSMTLQSEPPVEMITPGVWDVSQPSPVTLQIDFPATGWEYMKPDSEENRSNLEEPLKECIKHIAR
  LSQKQTPLLLSEEKKRYLWFYRFYCNNENCSLPLVLGSAPGWDERTVSEMHTILRRWTFSQPLEALGLLT
  SSFPDQEIRKVAVQQLDNLLNDELLEYLPQLVQAVKFEWNLESPLVQLLLHRSLQSIQVAHRLYWLLKNA
  ENEAYFKSWYQKLLAALQFCAGKALNDEFSKEQKLIKILGDIGERVKSASDHQRQEVLKKEIGRLEEFFQ
  DVNTCHLPLNPALCIKGIDHDACSYFTSNALPLKITFINANPMGKNISIIFKAGDDLRQDMLVLQLIQVM
  DNIWLQEGLDMQMIIYRCLSTGKDQGLVQMVPDAVTLAKIHRHSGLIGPLKENTIKKWFSQHNHLKADYE
  KALRNFFYSCAGWCVVTFILGVCDRHNDNIMLTKSGHMFHIDFGKFLGHAQTFGGIKRDRAPFIFTSEME
  YFITEGGKNPQHFQDFVELCCRAYNIIRKHSQLLLNLLEMMLYAGLPELSGIQDLKYVYNNLRPQDTDLE
  ATSHFTKKIKESLECFPVKLNNLIHTLAQMSAISPAKSTSQTFPQESCLLSTTRSIERATILGFSKKSSN
  LYLIQVTHSNNETSLTEKSFEQFSKLHSQLQKQFASLTLPEFPHWWHLPFTNSDHRRFRDLNHYMEQILN
  VSHEVTNSDCVLSFFLSEAVQQTVEESSPVYLGEKFPDKKPKVQLVISYEDVKLTILVKHMKNIHLPDGS
  APSAHVEFYLLPYPSEVRRRKTKSVPKCTDPTYNEIVVYDEVTELQGHVLMLIVKSKTVFVGAINIRLCS
  VPLDKEKWYPLGNSII
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001288772
• ORF Size: 4458 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001288772.2
• RefSeq Size: 4971 bp
• RefSeq ORF: 4461 bp
• Locus ID: 5288
• UniProt ID: O75747
• Cytogenetics: 12p12.3
• Protein Families: Druggable Genome
• Protein Pathways: Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system
• MW: 171.1 kDa
• Gene Summary: The protein encoded by this gene belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. This gene may play a role in several diseases, including type II diabetes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]