SENP7 (NM_001282801) Human Tagged ORF Clone

CAT#: RC239853

SENP7 (myc-DDK-tagged) - Human SUMO1/sentrin specific peptidase 7 (SENP7), transcript variant 3

Product Images

Circular map for RC239853

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SENP7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239853 representing NM_001282801
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACAAGAGAAAGCTCGGGCGACGGCCATCTTCATCCGAAATCATCACAGAAGGAAAAAGGAAAAAGT
  CATCTTCTGATTTATCGGAGATAAGAAAGATGTTAAATGCAAAACCAGAGGATGTCCATGTTCAATCACC
  ACTGTCCAAATTCAGAAGCTCAGAACGCTGGACTCTCCCTTTGCAGTGGGAAAGAAGCCTAAGGAATAAA
  GTCATCTCTCTAGACCATAAAAATAAAAAACATATCCGAGGGTGTCCTGTTACTTCCAAGTCATCACCAG
  AAAGGATACCCAGAGTTATATTGACGAATGTCCTGGGAACGGAGTTAGGAAGAAAATACATAAGGACCCC
  ACCTGTAACTGAGGGAAGTTTGAGTGATACAGACAACTTGCAATCAGAGCAACTTTCTTCATCATCTGAT
  GGCAGCCTAGAATCTTATCAAAATCTAAACCCTCACAAGAGCTGTTATTTATCTGAAAGGGGCTCACAAC
  GAAGTAAGACAGTAGATGACAATTCTGCAAAGCAGACTGCGCACAATAAAGAAAAACGAAGAAAGGATGA
  TGGCATTTCTCTTTTAATATCTGATACTCAGCCTGAAGACCTTAACAGTGGAAGTAGAGGTTGTGATCAT
  CTCGAACAGGAAAGCAGAAACAAGGATGTTAAATATTCTGATTCAAAAGTGGAACTCACTCTGATTTCCA
  GGAAGACAAAGAGAAGGCTTAGAAATAATTTACCTGATTCTCAATATTGTACTTCTTTGGATAAGTCAAC
  AGAACAGACAAAAAAACAAGAAGATGACTCAACAATATCCACTGAGTTTGAAAAGCCAAGTGAAAACTAT
  CATCAGGATCCAAAACTGCCTGAAGAAATTACAACTAAACCTACAAAAAGTGATTTTACTAAACTATCCT
  CACTTAACAGTCAGGAGTTGACTTTGAGTAATGCCACCAAAAGTGCCTCTGCCGGTTCAACCACTGAAAC
  CGTTGAGAACTCTAATTCCATTGATATTGTGGGGATTTCTTCCCTGGTTGAGAAGGATGAGAATGAGTTG
  AATACCATAGAAAAGCCTATTCTAAGAGGACATAATGAAGGGAACCAATCACTGATCTCAGCTGAACCAA
  TTGTTGTTTCCAGTGATGAAGAAGGACCTGTTGAACATAAAAGTTCAGAAATTCTTAAGTTACAATCTAA
  GCAAGACCGTGAGACAACTAATGAAAATGAGAGTACTTCTGAATCAGCATTGTTAGAACTACCATTGATT
  ACATGTGAATCTGTACAGATGTCATCTGAATTATGCCCATATAATCCTGTCATGGAGAACATTTCCAGTA
  TTATGCCTAGTAATGAGATGGATCTACAACTGGATTTTATATTTACTTCTGTTTATATTGGTAAAATAAA
  AGGAGCTTCTAAAGGTTGTGTTACAATCACAAAAAAATATATTAAGATCCCATTTCAAGTGTCCCTGAAT
  GAGATTTCATTGCTAGTGGATACCACACATTTAAAGCGGTTTGGGTTATGGAAAAGTAAGGATGATAATC
  ACAGTAAAAGGAGTCATGCTATTCTTTTCTTCTGGGTCTCTTCAGATTATCTTCAAGAGATTCAGACCCA
  ATTAGAACACTCTGTATTAAGCCAGCAATCAAAATCTAGTGAATTCATTTTCCTTGAACTACACAATCCT
  GTTTCACAAAGAGAAGAATTGAAGCTGAAAGATATTATGACGGAAATAAGTATAATCAGTGGAGAATTAG
  AGCTTTCTTACCCGTTGTCTTGGGTTCAGGCATTTCCTTTGTTTCAGAACCTCTCTTCAAAAGAAAGTTC
  TTTTATTCATTATTACTGTGTTTCAACTTGTTCTTTCCCTGCTGGTGTTGCTGTTGCTGAAGAAATGAAG
  CTGAAATCAGTATCTCAGCCCTCAAACACAGATGCGGCCAAGCCTACTTACACCTTCCTGCAGAAGCAAA
  GTAGCGGTTGCTACTCCCTTTCTATTACATCTAATCCAGATGAAGAATGGCGAGAAGTCAGGCACACTGG
  ACTTGTTCAGAAGTTGATTGTATATCCTCCACCACCTACTAAGGGGGGATTAGGAGTAACTAATGAAGAT
  CTGGAGTGTTTAGAAGAAGGAGAGTTTCTTAATGATGTAATCATTGATTTTTACCTTAAGTATCTTATAT
  TGGAGAAGGCATCAGATGAACTTGTTGAACGAAGTCACATTTTTAGTAGCTTTTTCTATAAATGCTTGAC
  AAGAAAGGAAAATAATTTAACAGAAGATAATCCAAATCTTTCAATGGCACAGAGAAGACATAAAAGAGTA
  AGAACATGGACTCGTCACATAAACATTTTTAATAAAGATTACATCTTTGTACCTGTAAATGAGTCGTCTC
  ACTGGTATCTCGCAGTCATTTGTTTTCCATGGTTAGAAGAAGCTGTGTATGAAGATTTTCCACAAACTGT
  ATCCCAGCAGTCCCAGGCTCAGCAGTCCCAAAATGACAACAAAACAATAGATAATGATCTACGTACTACT
  TCGACACTGTCTTTGAGTGCAGAGGATTCCCAAAGTACCGAGTCGAATATGTCAGTACCAAAGAAAATGT
  GTAAAAGGCCATGTATTCTTATACTAGACTCCTTGAAAGCTGCTTCTGTACAAAACACAGTTCAGAATTT
  ACGAGAGTATTTAGAGGTAGAGTGGGAAGTTAAACTAAAAACTCATCGTCAATTCAGCAAAACAAACATG
  GTGGATCTATGCCCTAAAGTTCCTAAACAGGACAATAGCAGTGATTGTGGAGTATATTTATTGCAGTATG
  TGGAAAGCTTCTTCAAGGATCCTATTGTTAACTTTGAACTTCCAATTCATTTGGAGAAGTGGTTTCCTCG
  TCATGTAATAAAGACCAAACGGGAAGATATTCGAGAGCTCATCTTGAAACTTCATTTACAGCAACAGAAG
  GGCAGCAGTAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239853 representing NM_001282801
  Red=Cloning site Green=Tags(s)
  
  MDKRKLGRRPSSSEIITEGKRKKSSSDLSEIRKMLNAKPEDVHVQSPLSKFRSSERWTLPLQWERSLRNK
  VISLDHKNKKHIRGCPVTSKSSPERIPRVILTNVLGTELGRKYIRTPPVTEGSLSDTDNLQSEQLSSSSD
  GSLESYQNLNPHKSCYLSERGSQRSKTVDDNSAKQTAHNKEKRRKDDGISLLISDTQPEDLNSGSRGCDH
  LEQESRNKDVKYSDSKVELTLISRKTKRRLRNNLPDSQYCTSLDKSTEQTKKQEDDSTISTEFEKPSENY
  HQDPKLPEEITTKPTKSDFTKLSSLNSQELTLSNATKSASAGSTTETVENSNSIDIVGISSLVEKDENEL
  NTIEKPILRGHNEGNQSLISAEPIVVSSDEEGPVEHKSSEILKLQSKQDRETTNENESTSESALLELPLI
  TCESVQMSSELCPYNPVMENISSIMPSNEMDLQLDFIFTSVYIGKIKGASKGCVTITKKYIKIPFQVSLN
  EISLLVDTTHLKRFGLWKSKDDNHSKRSHAILFFWVSSDYLQEIQTQLEHSVLSQQSKSSEFIFLELHNP
  VSQREELKLKDIMTEISIISGELELSYPLSWVQAFPLFQNLSSKESSFIHYYCVSTCSFPAGVAVAEEMK
  LKSVSQPSNTDAAKPTYTFLQKQSSGCYSLSITSNPDEEWREVRHTGLVQKLIVYPPPPTKGGLGVTNED
  LECLEEGEFLNDVIIDFYLKYLILEKASDELVERSHIFSSFFYKCLTRKENNLTEDNPNLSMAQRRHKRV
  RTWTRHINIFNKDYIFVPVNESSHWYLAVICFPWLEEAVYEDFPQTVSQQSQAQQSQNDNKTIDNDLRTT
  STLSLSAEDSQSTESNMSVPKKMCKRPCILILDSLKAASVQNTVQNLREYLEVEWEVKLKTHRQFSKTNM
  VDLCPKVPKQDNSSDCGVYLLQYVESFFKDPIVNFELPIHLEKWFPRHVIKTKREDIRELILKLHLQQQK
  GSSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282801
• ORF Size: 2952 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001282801.2
• RefSeq Size: 4820 bp
• RefSeq ORF: 2955 bp
• Locus ID: 57337
• UniProt ID: Q9BQF6
• Cytogenetics: 3q12.3
• Protein Families: Druggable Genome, Protease
• MW: 112.6 kDa
• Gene Summary: The reversible posttranslational modification of proteins by the addition of small ubiquitin-like SUMO proteins (see SUMO1; MIM 601912) is required for many cellular processes. SUMO-specific proteases, such as SENP7, process SUMO precursors to generate a C-terminal diglycine motif required for the conjugation reaction. They also display isopeptidase activity for deconjugation of SUMO-conjugated substrates (Lima and Reverter, 2008 [PubMed 18799455]).[supplied by OMIM, Jun 2009]