TLE 1 (TLE1) (NM_001303103) Human Tagged ORF Clone

CAT#: RC239546

TLE1 (myc-DDK-tagged) - Human transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila) (TLE1), transcript variant 1

Product Images

Circular map for RC239546

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TLE 1
• Synonyms: ESG; ESG1; GRG1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239546 representing NM_001303103
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCCCGCAGAGCCGGCACCCGACGCCGCACCAGGCTGCAGGCCAGCCCTTCAAGTTCACTATCCCGG
  AGTCCCTGGACCGGATTAAAGAGGAATTCCAGTTCCTGCAGGCGCAGTATCACAGCCTTAAATTGGAATG
  TGAGAAACTGGCAAGTGAAAAGACAGAAATGCAGAGGCACTATGTGATGTATTATGAAATGTCATATGGA
  TTAAACATTGAAATGCACAAACAGACTGAAATCGCCAAGAGATTGAATACGATTTGTGCACAAGTCATCC
  CATTTCTGTCTCAGGAACATCAACAACAGGTGGCCCAGGCTGTTGAACGTGCCAAACAGGTGACCATGGC
  AGAATTGAATGCCATCATCGGGGTACGTGGCCTACCAGGTCTACCTCCTACACAGCAGCAGTTGCAAGCT
  CAGCATCTTTCTCATGGCCACGGACCCCCAGTTCCCCTTACGCCTCACCCTTCGGGACTTCAGCCTCCTG
  GAATCCCGCCCCTCGGGGGCAGTGCCGGCCTTCTTGCGCTGTCTAGTGCTCTGAGTGGGCAGTCTCACTT
  GGCAATAAAAGATGACAAGAAGCACCACGATGCAGAGCACCACAGAGACAGAGAGCCGGGCACAAGTAAT
  TCCCTCCTGGTCCCAGACAGTCTAAGAGGCACAGATAAACGCAGAAATGGACCTGAATTTTCCAATGACA
  TCAAGAAAAGGAAGGTGGATGATAAGGACTCCAGCCACTATGACAGTGATGGTGACAAAAGCGATGACAA
  CTTAGTTGTGGATGTGTCTAATGAGGACCCTTCTTCTCCGCGAGCAAGCCCTGCCCACTCGCCCCGGGAA
  AATGGAATCGACAAAAATCGCCTGCTAAAGAAGGATGCTTCTAGCAGTCCAGCTTCCACGGCCTCCTCGG
  CAAGTTCCACTTCTTTGAAATCCAAAGAAATGAGCTTGCATGAAAAAGCCAGCACGCCTGTTCTGAAATC
  CAGCACACCAACGCCTCGGAGCGACATGCCAACGCCGGGCACCAGCGCCACTCCAGGCCTCCGTCCAGGT
  CTCGGCAAGCCTCCAGCCATAGACCCCCTCGTTAACCAAGCGGCAGCTGGCTTGAGGACACCCCTGGCAG
  TGCCCGGCCCATATCCTGCTCCTTTTGGGATGGTCCCCCACGCTGGCATGAACGGCGAGCTGACCAGCCC
  AGGCGCTGCCTACGCCAGTTTACACAACATGTCGCCCCAGATGAGCGCCGCAGCCGCCGCGGCCGCCGTG
  GTGGCCTACGGGCGCTCCCCCATGGTGGGGTTTGATCCTCCCCCTCACATGAGAGTACCTACCATTCCTC
  CAAACCTGGCAGGAATCCCTGGGGGGAAACCTGCATACTCCTTCCACGTTACTGCAGACGGTCAGATGCA
  GCCTGTCCCTTTTCCCCCCGACGCCCTCATCGGACCCGGAATCCCCCGGCATGCTCGCCAGATCAACACC
  CTCAACCACGGGGAGGTGGTGTGCGCTGTGACCATCAGCAACCCCACGAGACACGTGTACACAGGCGGGA
  AGGGCTGCGTCAAGGTCTGGGACATCAGCCACCCTGGCAATAAGAGCCCTGTCTCCCAGCTCGACTGTCT
  GAACAGAGACAATTATATCCGTTCCTGTAAATTGCTACCCGATGGCTGCACTCTCATAGTGGGAGGGGAA
  GCCAGTACTTTGTCCATTTGGGACCTGGCGGCTCCAACCCCGCGCATCAAGGCGGAGCTGACGTCCTCGG
  CCCCCGCCTGCTACGCCCTGGCCATCAGCCCCGATTCCAAGGTCTGCTTCTCATGCTGCAGCGACGGCAA
  CATCGCTGTGTGGGATCTGCACAACCAGACACTAGTGAGGCAATTCCAGGGCCACACAGACGGAGCCAGC
  TGTATTGACATTTCTAATGATGGCACCAAGCTCTGGACGGGTGGTTTGGACAACACAGTCAGGTCCTGGG
  ACCTGCGCGAGGGGCGGCAGCTGCAGCAGCACGACTTCACCTCCCAGATCTTCTCCCTGGGGTACTGCCC
  CACCGGGGAGTGGCTGGCAGTGGGCATGGAGAGCAGCAATGTGGAGGTGCTGCACGTGAACAAGCCTGAC
  AAGTACCAGCTGCACCTGCATGAGAGCTGCGTGCTGTCCCTGAAATTTGCTTACTGTGGTAAATGGTTTG
  TGAGTACTGGAAAAGATAACCTCCTCAATGCTTGGCGGACCCCCTATGGAGCCAGCATATTCCAGTCCAA
  AGAGTCCTCGTCAGTGCTTAGCTGTGACATCTCTGTGGATGATAAGTACATAGTCACTGGCTCGGGGGAC
  AAGAAGGCTACAGTCTATGAAGTCATCTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239546 representing NM_001303103
  Red=Cloning site Green=Tags(s)
  
  MFPQSRHPTPHQAAGQPFKFTIPESLDRIKEEFQFLQAQYHSLKLECEKLASEKTEMQRHYVMYYEMSYG
  LNIEMHKQTEIAKRLNTICAQVIPFLSQEHQQQVAQAVERAKQVTMAELNAIIGVRGLPGLPPTQQQLQA
  QHLSHGHGPPVPLTPHPSGLQPPGIPPLGGSAGLLALSSALSGQSHLAIKDDKKHHDAEHHRDREPGTSN
  SLLVPDSLRGTDKRRNGPEFSNDIKKRKVDDKDSSHYDSDGDKSDDNLVVDVSNEDPSSPRASPAHSPRE
  NGIDKNRLLKKDASSSPASTASSASSTSLKSKEMSLHEKASTPVLKSSTPTPRSDMPTPGTSATPGLRPG
  LGKPPAIDPLVNQAAAGLRTPLAVPGPYPAPFGMVPHAGMNGELTSPGAAYASLHNMSPQMSAAAAAAAV
  VAYGRSPMVGFDPPPHMRVPTIPPNLAGIPGGKPAYSFHVTADGQMQPVPFPPDALIGPGIPRHARQINT
  LNHGEVVCAVTISNPTRHVYTGGKGCVKVWDISHPGNKSPVSQLDCLNRDNYIRSCKLLPDGCTLIVGGE
  ASTLSIWDLAAPTPRIKAELTSSAPACYALAISPDSKVCFSCCSDGNIAVWDLHNQTLVRQFQGHTDGAS
  CIDISNDGTKLWTGGLDNTVRSWDLREGRQLQQHDFTSQIFSLGYCPTGEWLAVGMESSNVEVLHVNKPD
  KYQLHLHESCVLSLKFAYCGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISVDDKYIVTGSGD
  KKATVYEVIY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001303103
• ORF Size: 2340 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001303103.1, NP_001290032.1
• RefSeq Size: 4167 bp
• RefSeq ORF: 2343 bp
• Locus ID: 7088
• UniProt ID: Q04724
• Cytogenetics: 9q21.32
• Protein Families: Transcription Factors
• MW: 84.6 kDa
• Gene Summary: Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG.[UniProtKB/Swiss-Prot Function]