TMC7 (NM_001300732) Human Tagged ORF Clone

CAT#: RC239499

TMC7 (myc-DDK-tagged) - Human transmembrane channel-like 7 (TMC7), transcript variant 3

Product Images

Circular map for RC239499

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TMC7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239499 representing NM_001300732
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGAGTCCAGCGGCAGTGCGCTCCAGCCCGGCAGGCCCAGCCGGCAGCCGGCGGTCCATCCAGAGA
  ACCTCTCTCTAGACTCCAGTTGCTTCTCTTCTCCACCTGTGAACTTCCTCCAAGAATTGCCAAGCTACCG
  GTCCATTGCACGTAGGAGAACGACTGTCCATTCCCGGGACAAGCAAAGCGGAACTTTGCTAAAGCCAACC
  GACTCTTACAGCTCCCAGCTGGAGGACAGAATCGCTGAAAACCTCAGCAGCCATTCTCTTCGAAATTATG
  CACTGAACATCTCTGAGAAGCGGAGACTAAGGGACATTCAAGAGACACAAATGAAGTATCTCTCCGAATG
  GGACCAGTGGAAGCGGTATAGCAGCAAGTCTTGGAAGAGGTTCCTAGAGAAGGCTCGAGAGATGACGACC
  CACCTGGAGCTGTGGCGGGAGGACATCCGCAGCATAGAAGGGAAATTTGGCACTGGGATTCAGTCCTATT
  TCTCCTTCTTGAGATTCCTGGTGTTGCTGAATTTGGTGATATTTCTGATCATCTTTATGCTGGTTTTGCT
  CCCAGTCTTACTCACGAAATACAAGATCACCAACAGCAGCTTCGTGCTCATTCCTTTCAAAGACATGGAT
  AAACAATGTACAGTCTATCCAGTAAGCAGTTCTGGACTCATTTACTTTTACAGTTATATCATAGACTTGC
  TTTCTGGCACTGGTTTCCTGGAGGAAACTAGCCTCTTTTACGGACATTACACCATTGATGGGGTGAAATT
  TCAGAACTTCACCTATGATCTGCCCCTGGCGTATTTGTTAAGCACAATCGCCTCCCTGGCCCTGAGCCTT
  CTTTGGATAGTGAAAAGGTCGGTGGAAGGATTCAAAATCAACCTGATTCGGAGTGAGGAGCACTTTCAGA
  GTTACTGCAACAAGATATTTGCCGGCTGGGACTTCTGCATCACTAACCGCAGCATGGCGGATCTGAAGCA
  CAGCAGCTTGCGGTACGAGCTCCGAGCAGATCTGGAGGAAGAAAGAATGCGGCAGAAAATAGCAGAAAGG
  ACCTCAGAAGAAACAATACGCATTTACTCTTTGAGACTGTTTTTGAACTGTATTGTTCTGGCTGTTTTAG
  GGGCATGCTTTTATGCAATATACGTAGCAACTGTCTTCTCGCAAGAGCACATGAAAAAGGAAATCGACAA
  GATGGTTTTTGGAGAGAACCTCTTCATATTGTATCTACCGTCTATTGTGATCACGCTGGCCAATTTTATC
  ACCCCAATGATCTTTGCCAAGATCATCCGCTATGAGGATTATTCTCCAGGCTTTGAGATCCGTCTGACAA
  TCCTTAGGTGTGTCTTTATGCGGCTGGCCACCATATGTGTCCTGGTGTTCACGCTGGGCTCCAAGATCAC
  ATCCTGTGATGATGACACATGTGACCTTTGCGGCTACAACCAGAAACTCTACCCGTGCTGGGAGACCCAA
  GTTGGGCAGGAAATGTACAAGCTGATGATCTTCGACTTCATCATCATCTTGGCTGTGACACTCTTCGTGG
  ATTTTCCTAGAAAGCTCCTGGTGACCTACTGTTCCTCTTGCAAGCTGATTCAGTGCTGGGGGCAGCAGGA
  GTTTGCCATTCCTGATAACGTCCTGGGGATAGTTTACGGGCAAACCATCTGCTGGATCGGAGCCTTTTTC
  TCACCCCTTCTCCCTGCAATTGCAACCCTGAAATTCATTATCATCTTCTATGTGAAAGAGTGGAGTCTGC
  TTTACACCTGCAGACCCTCCCCCAGGCCGTTCAGAGCATCCAATTCTAATTTCTTCTTCCTGTTGGTGTT
  GTTGATCGGGCTGTGTTTGGCAATAATACCTCTGACAATCAGCATATCACGCATCCCTTCCTCGAAAGCC
  TGTGGGCCGTTCACCAACTTCAACACCACCTGGGAGGTCATCCCCAAGACGGTGAGCACCTTCCCCAGCT
  CGCTGCAGTCCTTCATCCATGGTGTCACATCCGAAGCCTTTGCAGTTCCTTTCTTCATGATTATTTGCCT
  CATCATGTTTTACTTCATTGCCTTAGCTGGAGCACACAAACGGGTGGTCATCCAGCTCCGAGAGCAGCTA
  TCCCTGGTAAGGAAGCATAGCTCCAGAGGGTCATGGCTGGGGACATTTACCCAGGACACTGCAAGGAAAG
  TGCTGTTTTCCTGGGAGGGAGTGAAATTACTGCAGCCTTCTCTCACCACCACCTCCCCAAAACTCTGCCT
  TGAGGCCCAGCCGCGTCCAGCTTCATTAATTCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239499 representing NM_001300732
  Red=Cloning site Green=Tags(s)
  
  MSESSGSALQPGRPSRQPAVHPENLSLDSSCFSSPPVNFLQELPSYRSIARRRTTVHSRDKQSGTLLKPT
  DSYSSQLEDRIAENLSSHSLRNYALNISEKRRLRDIQETQMKYLSEWDQWKRYSSKSWKRFLEKAREMTT
  HLELWREDIRSIEGKFGTGIQSYFSFLRFLVLLNLVIFLIIFMLVLLPVLLTKYKITNSSFVLIPFKDMD
  KQCTVYPVSSSGLIYFYSYIIDLLSGTGFLEETSLFYGHYTIDGVKFQNFTYDLPLAYLLSTIASLALSL
  LWIVKRSVEGFKINLIRSEEHFQSYCNKIFAGWDFCITNRSMADLKHSSLRYELRADLEEERMRQKIAER
  TSEETIRIYSLRLFLNCIVLAVLGACFYAIYVATVFSQEHMKKEIDKMVFGENLFILYLPSIVITLANFI
  TPMIFAKIIRYEDYSPGFEIRLTILRCVFMRLATICVLVFTLGSKITSCDDDTCDLCGYNQKLYPCWETQ
  VGQEMYKLMIFDFIIILAVTLFVDFPRKLLVTYCSSCKLIQCWGQQEFAIPDNVLGIVYGQTICWIGAFF
  SPLLPAIATLKFIIIFYVKEWSLLYTCRPSPRPFRASNSNFFFLLVLLIGLCLAIIPLTISISRIPSSKA
  CGPFTNFNTTWEVIPKTVSTFPSSLQSFIHGVTSEAFAVPFFMIICLIMFYFIALAGAHKRVVIQLREQL
  SLVRKHSSRGSWLGTFTQDTARKVLFSWEGVKLLQPSLTTTSPKLCLEAQPRPASLIH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001300732
• ORF Size: 2274 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001300732.1, NP_001287661.1
• RefSeq Size: 2738 bp
• RefSeq ORF: 2277 bp
• Locus ID: 79905
• Cytogenetics: 16p12.3
• Protein Families: Transmembrane
• MW: 87.6 kDa
• Gene Summary: Probable ion channel.[UniProtKB/Swiss-Prot Function]