OSBP2 (NM_001282741) Human Tagged ORF Clone

CAT#: RC239241

OSBP2 (myc-DDK-tagged) - Human oxysterol binding protein 2 (OSBP2), transcript variant 6

Product Images

Circular map for RC239241

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: OSBP2
• Synonyms: HLM; ORP-4; ORP4; OSBPL1; OSBPL4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239241 representing NM_001282741
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGGACGCGGCTGCTGGGACACGGCCTCCGTGCGCTCCTTCTGCAGCTCCGGCTGCCTGAATAAAT
  TTAAGAAGGATGACTCTGGGGACGACGACGAGGCTACCACCCCAGCCGACAAGAGCGAGCTGCACCACAC
  CCTGAAGAATCTTTCCCTGAAGTTAGATGACCTCAGCACGTGCAATGACCTCATCGCCAAGCACGGCGCT
  GCACTCCAGCGCTCCCTGACAGAGCTGGACGGCCTCAAGATCCCATCTGAGAGTGGGGAGAAGCTGAAGG
  TGGTGAATGAGCGGGCCACCCTCTTCCGCATCACATCCAATGCTATGATCAACGCCTGCAGGGACTTCTT
  GGAACTAGCAGAGATACACAGTCGGAAATGGCAGCGGGCACTGCAGTATGAGCAGGAGCAGCGCGTGCAC
  TTGGAGGAAACCATTGAGCAGCTGGCGAAGCAGCACAACAGCCTCGAGCGGGCCTTCCACAGTGCCCCTG
  GCCGGCCGGCCAACCCCTCCAAGAGCTTCATTGAGGGAAGCCTCTTGACTCCCAAAGGAGAGGACAGTGA
  GGAAGATGAAGATACCGAGTACTTTGATGCCATGGAAGACTCCACATCCTTCATCACCGTGATCACCGAG
  GCCAAGGAAGACAGCAGAAAAGCTGAAGGTAGCACCGGGACAAGTTCCGTGGACTGGAGCTCAGCAGACA
  ATGTACTAGATGGTGCCTCGCTCGTGCCCAAGGGTTCATCCAAAGTCAAGAGGCGAGTCCGCATTCCCAA
  CAAGCCCAACTACAGCCTTAACCTCTGGAGCATCATGAAGAACTGCATCGGCCGGGAGCTCTCCAGGATC
  CCCATGCCGGTGAACTTCAATGAGCCCCTGTCCATGCTCCAGCGGCTGACAGAGGACCTGGAGTACCACC
  ACCTGCTGGACAAGGCAGTGCACTGCACCAGCTCAGTGGAGCAGATGTGCCTGGTGGCCGCCTTCTCTGT
  GTCCTCCTACTCCACCACAGTGCACCGCATCGCCAAGCCCTTCAACCCCATGCTGGGGGAGACCTTCGAG
  CTGGACCGCCTCGACGACATGGGCCTGCGCTCCCTCTGTGAGCAGGTGAGCCACCACCCCCCCTCAGCTG
  CGCACTACGTGTTCTCCAAGCATGGCTGGAGCCTCTGGCAGGAGATCACCATCTCCAGCAAGTTCCGGGG
  AAAATACATCTCCATCATGCCGCTAGGTGCCATCCACTTAGAATTCCAGGCCAGTGGGAATCACTACGTG
  TGGAGGAAGAGCACCTCAACTGTTCACAACATCATCGTGGGCAAGCTCTGGATCGACCAGTCAGGGGACA
  TCGAGATTGTGAACCATAAGACCAATGACCGGTGCCAGCTGAAGTTCCTGCCCTACAGCTACTTCTCCAA
  AGAGGCAGCCCGGAAGGTGACAGGAGTGGTGAGTGACAGCCAGGGCAAGGCCCATTACGTGCTGTCCGGC
  TCGTGGGATGAACAAATGGAGTGCTCCAAGGTCATGCATAGCAGTCCCAGCAGCCCCAGCTCTGACGGGA
  AGCAGAAGACAGTGTACCAGACCCTGTCAGCCAAGCTGCTGTGGAAGAAGTACCCGCTGCCGGAGAACGC
  GGAGAACATGTACTACTTCTCAGAGCTGGCCCTGACCCTCAACGAGCACGAGGAGGGCGTAGCGCCAACC
  GACAGCCGCCTGCGGCCCGACCAGCGGCTGATGGAGAAGGGCCGTTGGGACGAGGCCAATACCGAGAAGC
  AGCGGCTGGAGGAGAAGCAGCGCCTGTCGCGGCGCCGGCGGCTGGAGGCCTGCGGGCCGGGCAGCAGCTG
  CAGCTCGGAGGAAGCAGAGAAGGAGGCGGATGCCTACACGCCACTGTGGTTTGAGAAGAGGCTGGATCCG
  CTGACCGGGGAGATGGCCTGTGTGTACAAGGGCGGCTACTGGGAGGCCAAGGAGAAGCAAGACTGGCATA
  TGTGCCCCAACATCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239241 representing NM_001282741
  Red=Cloning site Green=Tags(s)
  
  MKGRGCWDTASVRSFCSSGCLNKFKKDDSGDDDEATTPADKSELHHTLKNLSLKLDDLSTCNDLIAKHGA
  ALQRSLTELDGLKIPSESGEKLKVVNERATLFRITSNAMINACRDFLELAEIHSRKWQRALQYEQEQRVH
  LEETIEQLAKQHNSLERAFHSAPGRPANPSKSFIEGSLLTPKGEDSEEDEDTEYFDAMEDSTSFITVITE
  AKEDSRKAEGSTGTSSVDWSSADNVLDGASLVPKGSSKVKRRVRIPNKPNYSLNLWSIMKNCIGRELSRI
  PMPVNFNEPLSMLQRLTEDLEYHHLLDKAVHCTSSVEQMCLVAAFSVSSYSTTVHRIAKPFNPMLGETFE
  LDRLDDMGLRSLCEQVSHHPPSAAHYVFSKHGWSLWQEITISSKFRGKYISIMPLGAIHLEFQASGNHYV
  WRKSTSTVHNIIVGKLWIDQSGDIEIVNHKTNDRCQLKFLPYSYFSKEAARKVTGVVSDSQGKAHYVLSG
  SWDEQMECSKVMHSSPSSPSSDGKQKTVYQTLSAKLLWKKYPLPENAENMYYFSELALTLNEHEEGVAPT
  DSRLRPDQRLMEKGRWDEANTEKQRLEEKQRLSRRRRLEACGPGSSCSSEEAEKEADAYTPLWFEKRLDP
  LTGEMACVYKGGYWEAKEKQDWHMCPNIF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282741
• ORF Size: 1977 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001282741.1, NP_001269670.1
• RefSeq Size: 3589 bp
• RefSeq ORF: 1980 bp
• Locus ID: 23762
• UniProt ID: Q969R2
• Cytogenetics: 22q12.2
• MW: 75.1 kDa
• Gene Summary: The protein encoded by this gene contains a pleckstrin homology (PH) domain and an oxysterol-binding region. It binds oxysterols such as 7-ketocholesterol and may inhibit their cytotoxicity. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2013]