ASB15 (NM_001290258) Human Tagged ORF Clone

CAT#: RC238999

ASB15 (myc-DDK-tagged) - Human ankyrin repeat and SOCS box containing 15 (ASB15), transcript variant 2

Product Images

Circular map for RC238999

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ASB15
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC238999 representing NM_001290258
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATACTAATGATGACCCTGATGAAGACCATCTTACAAGTTATGATATTCAGCTAAGTATTCAAGAAT
  CCATTGAAGCCAGCAAGACTGCACTTTGTCCTGAAAGATTTGTACCCCTAAGTGCTCAAAACAGAAAACT
  TGTGGAGGCCATAAAACAAGGTCACATTCCTGAGCTCCAGGAGTATGTAAAATATAAATATGCAATGGAT
  GAAGCTGATGAAAAAGGATGGTTTCCATTGCATGAAGCTGTTGTTCAACCCATTCAACAAATACTTGAGA
  TTGTTCTGGATGCATCCTATAAGACACTCTGGGAATTCAAGACCTGTGATGGAGAAACACCCTTGACTTT
  GGCAGTCAAAGCTGGTCTGGTGGAAAATGTAAGAACTTTATTAGAAAAGGGAGTGTGGCCCAACACAAAA
  AATGATAAAGGAGAGACCCCCCTTCTGATTGCTGTGAAAAAGGGCTCCTATGACATGGTGTCGACTCTGA
  TCAAACATAACACTAGCCTAGACCAGCCCTGTGTCAAGCGATGGTCAGCAATGCATGAAGCAGCCAAGCA
  AGGCCGAAAAGATATCGTAGCTCTGCTGCTGAAACATGGAGGCAATGTCCACCTGAGAGATGGATTTGGA
  GTCACACCACTAGGCGTCGCTGCCGAGTATGGTCACTGTGACGTGTTAGAACATCTAATCCACAAAGGTG
  GTGATGTGCTTGCTTTGGCGGATGATGGGGCGTCGGTGCTGTTTGAGGCAGCAGGAGGTGGCAATCCCGA
  CTGCATTTCCCTCCTGCTGGAATATGGAGGAAGCGGAAATGTACCTAACCGAGCAGGACATCTTCCTATA
  CACCGAGCTGCCTATGAGGGGCATTATCTTGCACTGAAATATCTTATCCCAGTAACATCTAAAAATGCAA
  TTCGGAAAAGTGGGCTAACACCAATTCACTCAGCAGCAGATGGACAAAATGCACAGTGTCTAGAACTGCT
  CATTGAAAATGGTTTTGATGTCAACACTCTACTTGCTGACCACATTTCCCAGAGCTATGACGATGAGAGG
  AAGACTGCGCTGTATTTTGGCGTTTCTAATAATGACGTTCATTGCACAGAAGTCCTTCTGGCTGCAGGTG
  CAGACCCAAACTTAGATCCCCTCAACTGTCTACTTGTTGCAGTGAGGGCCAATAATTATGAAATTGTCAG
  GCTGCTTCTCTCCCATGGAGCTAATGTCAATTGTTATTTTATGCATGTGAATGACACTCGTTTCCCCAGT
  GTCATTCAATATGCTCTAAACGACGAGGTAATGCTGAGGCTATTGCTGAATAATGGCTATCAAGTGGAGA
  TGTGCTTTGACTGCATGCATGGTGACATCTTTGGAAATTCATTTGTGTGGTCAGAGATACAGGAAGAGGT
  GCTGCCAGGATGGACATCTTGTGTAATAAAAGATAACCCGTTCTGTGAGTTTATTACAGTTCCTTGGATG
  AAGCACTTGGTAGGCAGAGTTACTCGTGTACTAATAGATTACATGGATTATGTTCCTCTGTGTGCTAAAC
  TGAAGTCTGCACTAGAAGTACAGAGAGAATGGCCAGAAATCCGCCAAATACTAGAGAATCCTTGTTCATT
  GAAGCATTTGTGTCGGTTAAAAATTCGAAGGCTTATGGGTCTCCAGAAACTCTGCCAGCCAGCCTCAGTG
  GAGAAGCTTCCTCTACCACCAGCTATTCAAAGATACATATTATTTAAAGAGTATGATCTCTATGGACAAG
  AGCTAAAATTGACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC238999 representing NM_001290258
  Red=Cloning site Green=Tags(s)
  
  MDTNDDPDEDHLTSYDIQLSIQESIEASKTALCPERFVPLSAQNRKLVEAIKQGHIPELQEYVKYKYAMD
  EADEKGWFPLHEAVVQPIQQILEIVLDASYKTLWEFKTCDGETPLTLAVKAGLVENVRTLLEKGVWPNTK
  NDKGETPLLIAVKKGSYDMVSTLIKHNTSLDQPCVKRWSAMHEAAKQGRKDIVALLLKHGGNVHLRDGFG
  VTPLGVAAEYGHCDVLEHLIHKGGDVLALADDGASVLFEAAGGGNPDCISLLLEYGGSGNVPNRAGHLPI
  HRAAYEGHYLALKYLIPVTSKNAIRKSGLTPIHSAADGQNAQCLELLIENGFDVNTLLADHISQSYDDER
  KTALYFGVSNNDVHCTEVLLAAGADPNLDPLNCLLVAVRANNYEIVRLLLSHGANVNCYFMHVNDTRFPS
  VIQYALNDEVMLRLLLNNGYQVEMCFDCMHGDIFGNSFVWSEIQEEVLPGWTSCVIKDNPFCEFITVPWM
  KHLVGRVTRVLIDYMDYVPLCAKLKSALEVQREWPEIRQILENPCSLKHLCRLKIRRLMGLQKLCQPASV
  EKLPLPPAIQRYILFKEYDLYGQELKLT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001290258
• ORF Size: 1764 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290258.2
• RefSeq Size: 2975 bp
• RefSeq ORF: 1767 bp
• Locus ID: 142685
• UniProt ID: Q8WXK1
• Cytogenetics: 7q31.32
• Protein Families: Druggable Genome
• MW: 65.8 kDa
• Gene Summary: This gene encodes a member of the suppressor of cytokine signaling box superfamily. The proteins in this superfamily participate in the ubiquitin-proteasome system for the degradation of proteins in the cell cycle and signal transduction pathways. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]