HEPACAM2 (NM_001288810) Human Tagged ORF Clone

CAT#: RC238329

HEPACAM2 (myc-DDK-tagged) - Human HEPACAM family member 2 (HEPACAM2), transcript variant 4

Product Images

Circular map for RC238329

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HEPACAM2
• Synonyms: MIKI
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC238329 representing NM_001288810
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGCTCAAGGTCTTCACAACTTTCCTTTCCTTTGCAACAGGTGCTTGCTCGGGGCTGAAGGTGACAG
  TGCCATCACACACTGTCCATGGCGTCAGAGGTCAGGCCCTCTACCTACCCGTCCACTATGGCTTCCACAC
  TCCAGCATCAGACATCCAGATCATATGGCTATTTGAGAGACCCCACACAATGCCCAAATACTTACTGGGC
  TCTGTGAATAAGTCTGTGGTTCCTGACTTGGAATACCAACACAAGTTCACCATGATGCCACCCAATGCAT
  CTCTGCTTATCAACCCACTGCAGTTCCCTGATGAAGGCAATTACATCGTGAAGGTCAACATTCAGGGAAA
  TGGAACTCTATCTGCCAGTCAGAAGATACAAGTCACGGTTGATGATCCTGTCACAAAGCCAGTGGTGCAG
  ATTCATCCTCCCTCTGGGGCTGTGGAGTATGTGGGGAACATGACCCTGACATGCCATGTGGAAGGGGGCA
  CTCGGCTAGCTTACCAATGGCTAAAAAATGGGAGACCTGTCCACACCAGCTCCACCTACTCCTTTTCTCC
  CCAAAACAATACCCTTCATATTGCTCCAGTAACCAAGGAAGACATTGGGAATTACAGCTGCCTGGTGAGG
  AACCCTGTCAGTGAAATGGAAAGTGATATCATTATGCCCATCATATATTATGGACCTTATGGACTTCAAG
  TGAATTCTGATAAAGGGCTAAAAGTAGGGGAAGTGTTTACTGTTGACCTTGGAGAGGCCATCCTATTTGA
  TTGTTCTGCTGATTCTCATCCCCCCAACACCTACTCCTGGATTAGGAGGACTGACAATACTACATATATC
  ATTAAGCATGGGCCTCGCTTAGAAGTTGCATCTGAGAAAGTAGCCCAGAAGACAATGGACTATGTGTGCT
  GTGCTTACAACAACATAACCGGCAGGCAAGATGAAACTCATTTCACAGTTATCATCACTTCCGTAGGACT
  GGAGAAGCTTGCACAGAAAGGAAAATCATTGTCACCTTTAGCAAGTATAACTGGAATATCACTATTTTTG
  ATTATATCCATGTGTCTTCTCTTCCTATGGAAAAAATATCAACCCTACAAAGGCCAGAAACAGAATACAG
  GAAAGCTCAAACATTTTCAGGCCATGAAGATGCTCTGGATGACTTCGGAATATATGAATTTGTTGCTTTT
  CCAGATGTTTCTGGTGTTTCCAGGATCCCAAGCAGGTCTGTTCCAGCCTCTGATTGTGTATCGGGGCAAG
  ATTTGCACAGTACAGTGTATGAAGTTATTCAGCACATCCCTGCCCAGCAGCAAGACCATCCAGAGTGAAC
  TTTCATGGGCTAAACAGTACATTCGAGTGAAATTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC238329 representing NM_001288810
  Red=Cloning site Green=Tags(s)
  
  MWLKVFTTFLSFATGACSGLKVTVPSHTVHGVRGQALYLPVHYGFHTPASDIQIIWLFERPHTMPKYLLG
  SVNKSVVPDLEYQHKFTMMPPNASLLINPLQFPDEGNYIVKVNIQGNGTLSASQKIQVTVDDPVTKPVVQ
  IHPPSGAVEYVGNMTLTCHVEGGTRLAYQWLKNGRPVHTSSTYSFSPQNNTLHIAPVTKEDIGNYSCLVR
  NPVSEMESDIIMPIIYYGPYGLQVNSDKGLKVGEVFTVDLGEAILFDCSADSHPPNTYSWIRRTDNTTYI
  IKHGPRLEVASEKVAQKTMDYVCCAYNNITGRQDETHFTVIITSVGLEKLAQKGKSLSPLASITGISLFL
  IISMCLLFLWKKYQPYKGQKQNTGKLKHFQAMKMLWMTSEYMNLLLFQMFLVFPGSQAGLFQPLIVYRGK
  ICTVQCMKLFSTSLPSSKTIQSELSWAKQYIRVKF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001288810
• ORF Size: 1365 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001288810.3
• RefSeq Size: 2123 bp
• RefSeq ORF: 1368 bp
• Locus ID: 253012
• UniProt ID: A8MVW5
• Cytogenetics: 7q21.2
• Protein Families: Transmembrane
• MW: 51.5 kDa
• Gene Summary: This gene encodes a protein related to the immunoglobulin superfamily that plays a role in mitosis. Knockdown of this gene results in prometaphase arrest, abnormal nuclear morphology and apoptosis. Poly(ADP-ribosylation) of the encoded protein promotes its translocation to centrosomes, which may stimulate centrosome maturation. A chromosomal deletion including this gene may be associated with myeloid leukemia and myelodysplastic syndrome in human patients. [provided by RefSeq, Oct 2016]