ZNF302 (NM_001289181) Human Tagged ORF Clone

CAT#: RC238263

ZNF302 (myc-DDK-tagged) - Human zinc finger protein 302 (ZNF302), transcript variant 3

Product Images

Circular map for RC238263

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF302
• Synonyms: HSD16; MST154; MSTP154; ZNF135L; ZNF140L; ZNF327
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC238263 representing NM_001289181
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCAGCGTCAGTACCCAGAGTGTTACCTGGCACCAAATGGATGCTTAGTAAGTAATTGTGGAGTGA
  ATAAGATGAGTAATGAAGAACTGGTGGGGCAGAATCATGGCATGGAAGGTGAAGCTTGCACAGGAGGGGA
  TGTGACATTTAGTGATGTGGCTATAGACTTCTCTCATGAAGAGTGGGCATGCCTAGATTCTGCTCAGAGG
  GACTTATACAAGGATGTGATGGTCCAGAATTATGAGAACCTGGTCTCTGTAGGTCTTTCCGTAACTAAGC
  CATATGTGATCATGTTATTGGAGGATGGAAAAGAGCCCTGGATGATGGAGAAAAAACTGTCAAAAGATTG
  GGAATCAAGATGGGAAAACAAGGAATTATCAACAAAGAAGGATATTTATGATGAAGATTCACCCCAACCA
  GTAACAATGGAAAAAGTTGTAAAACAAAGTTATGAATTTTCAAATTCTAATAAGAATTTGGAATATACAG
  AATGCGACACATTTAGAAGCACCTTTCATTCAAAGTCTACTCTTTCTGAACCACAAAACAATTCTGCTGA
  AGGGAATTCACACAAATATGATATATTAAAGAAGAATTTATCAAAAAAGTCAGTTATAAAAAGTGAGAGA
  ATAAATGGTGGAAAGAAACTTTTAAATTCTAATAAAAGTGGGGCAGCCTTCAACCAGAGCAAATCTCTTA
  CCCTTCCCCAGACTTGTAATAGAGAGAAAATCTATACATGCAGTGAATGTGGGAAAGCCTTTGGCAAACA
  GTCAATCCTCAGTCGCCACTGGAGAATTCATACAGGAGAGAAGCCCTATGAATGTCGTGAATGTGGGAAG
  ACTTTTAGCCATGGTTCATCCCTTACACGACATCAGATAAGCCATAGTGGAGAGAAACCTTACAAATGCA
  TTGAATGTGGGAAGGCCTTTAGCCATGGCTCATCACTTACTAACCATCAGAGCACTCACACGGGAGAGAA
  ACCGTATGAATGTATGAACTGTGGAAAGTCTTTTAGTCGTGTGTCCCTTCTCATTCAGCATCTAAGAATT
  CATACGCAAGAAAAACGCTATGAGTGTCGTATATGTGGAAAGGCCTTCATTCATAGTTCGTCTCTCATTC
  ACCATCAGAAAAGCCATACTGGAGAGAAGCCTTATGAATGTAGAGAATGTGGGAAAGCTTTCTGCTGTAG
  CTCACACCTTACTCAACATCAAAGAATTCACAGTATGAAGAAAAAATATGAATGCAACAAATGTCTCAAG
  GTCTTTAGTAGCTTCTCATTTCTTGTTCAACATCAGAGTATTCATACTGAAGAAAAACCGTTTGAAGTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC238263 representing NM_001289181
  Red=Cloning site Green=Tags(s)
  
  MSQRQYPECYLAPNGCLVSNCGVNKMSNEELVGQNHGMEGEACTGGDVTFSDVAIDFSHEEWACLDSAQR
  DLYKDVMVQNYENLVSVGLSVTKPYVIMLLEDGKEPWMMEKKLSKDWESRWENKELSTKKDIYDEDSPQP
  VTMEKVVKQSYEFSNSNKNLEYTECDTFRSTFHSKSTLSEPQNNSAEGNSHKYDILKKNLSKKSVIKSER
  INGGKKLLNSNKSGAAFNQSKSLTLPQTCNREKIYTCSECGKAFGKQSILSRHWRIHTGEKPYECRECGK
  TFSHGSSLTRHQISHSGEKPYKCIECGKAFSHGSSLTNHQSTHTGEKPYECMNCGKSFSRVSLLIQHLRI
  HTQEKRYECRICGKAFIHSSSLIHHQKSHTGEKPYECRECGKAFCCSSHLTQHQRIHSMKKKYECNKCLK
  VFSSFSFLVQHQSIHTEEKPFEV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001289181
• ORF Size: 1329 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289181.1, NP_001276110.1
• RefSeq Size: 2820 bp
• RefSeq ORF: 1332 bp
• Locus ID: 55900
• UniProt ID: Q9NR11
• Cytogenetics: 19q13.11
• Protein Families: Transcription Factors
• MW: 51 kDa
• Gene Summary: This gene encodes a member of the zinc-finger protein family. The encoded protein contains seven C2H2-type zinc fingers and a KRAB domain, but its function has yet to be determined. Alternatively spliced transcript variants have been described. [provided by RefSeq, Mar 2014]