FADS2 (NM_001281501) Human Tagged ORF Clone

CAT#: RC238137

FADS2 (myc-DDK-tagged) - Human fatty acid desaturase 2 (FADS2), transcript variant 2

Product Images

Circular map for RC238137

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FADS2
• Synonyms: D6D; DES6; FADSD6; LLCDL2; SLL0262; TU13
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC238137 representing NM_001281501
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACGGCAGGGAGGCGGGACCCTTTGTTTGTGTGTGCGTGTTGTTGGCCTCCATCCCCACTCCCCAGA
  CTCCACTTCTCCAGGCCTCTCTCCCGCCTTTTCATCCCGCATCCGCAGGACACCCAATCACCGGGCAACA
  GGATGCCTTCCGCGCCTTCCACCCTGACCTGGAATTCGTGGGCAAGTTCTTGAAACCCCTGCTGATTGGT
  GAACTGGCCCCGGAGGAGCCCAGCCAGGACCACGGCAAGAACTCAAAGATCACTGAGGACTTCCGGGCCC
  TGAGGAAGACGGCTGAGGACATGAACCTGTTCAAGACCAACCACGTGTTCTTCCTCCTCCTCCTGGCCCA
  CATCATCGCCCTGGAGAGCATTGCATGGTTCACTGTCTTTTACTTTGGCAATGGCTGGATTCCTACCCTC
  ATCACGGCCTTTGTCCTTGCTACCTCTCAGGCCCAAGCTGGATGGCTGCAACATGATTATGGCCACCTGT
  CTGTCTACAGAAAACCCAAGTGGAACCACCTTGTCCACAAATTCGTCATTGGCCACTTAAAGGGTGCCTC
  TGCCAACTGGTGGAATCATCGCCACTTCCAGCACCACGCCAAGCCTAACATCTTCCACAAGGATCCCGAT
  GTGAACATGCTGCACGTGTTTGTTCTGGGCGAATGGCAGCCCATCGAGTACGGCAAGAAGAAGCTGAAAT
  ACCTGCCCTACAATCACCAGCACGAATACTTCTTCCTGATTGGGCCGCCGCTGCTCATCCCCATGTATTT
  CCAGTACCAGATCATCATGACCATGATCGTCCATAAGAACTGGGTGGACCTGGCCTGGGCCGTCAGCTAC
  TACATCCGGTTCTTCATCACCTACATCCCTTTCTACGGCATCCTGGGAGCCCTCCTTTTCCTCAACTTCA
  TCAGGTTCCTGGAGAGCCACTGGTTTGTGTGGGTCACACAGATGAATCACATCGTCATGGAGATTGACCA
  GGAGGCCTACCGTGACTGGTTCAGTAGCCAGCTGACAGCCACCTGCAACGTGGAGCAGTCCTTCTTCAAC
  GACTGGTTCAGTGGACACCTTAACTTCCAGATTGAGCACCACCTCTTCCCCACCATGCCCCGGCACAACT
  TACACAAGATCGCCCCGCTGGTGAAGTCTCTATGTGCCAAGCATGGCATTGAATACCAGGAGAAGCCGCT
  ACTGAGGGCCCTGCTGGACATCATCAGGTCCCTGAAGAAGTCTGGGAAGCTGTGGCTGGACGCCTACCTT
  CACAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC238137 representing NM_001281501
  Red=Cloning site Green=Tags(s)
  
  MHGREAGPFVCVCVLLASIPTPQTPLLQASLPPFHPASAGHPITGQQDAFRAFHPDLEFVGKFLKPLLIG
  ELAPEEPSQDHGKNSKITEDFRALRKTAEDMNLFKTNHVFFLLLLAHIIALESIAWFTVFYFGNGWIPTL
  ITAFVLATSQAQAGWLQHDYGHLSVYRKPKWNHLVHKFVIGHLKGASANWWNHRHFQHHAKPNIFHKDPD
  VNMLHVFVLGEWQPIEYGKKKLKYLPYNHQHEYFFLIGPPLLIPMYFQYQIIMTMIVHKNWVDLAWAVSY
  YIRFFITYIPFYGILGALLFLNFIRFLESHWFVWVTQMNHIVMEIDQEAYRDWFSSQLTATCNVEQSFFN
  DWFSGHLNFQIEHHLFPTMPRHNLHKIAPLVKSLCAKHGIEYQEKPLLRALLDIIRSLKKSGKLWLDAYL
  HK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001281501
• ORF Size: 1266 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001281501.1, NP_001268430.1
• RefSeq Size: 3025 bp
• RefSeq ORF: 1269 bp
• Locus ID: 9415
• UniProt ID: O95864
• Cytogenetics: 11q12.2
• Protein Families: Transmembrane
• Protein Pathways: alpha-Linolenic acid metabolism, Biosynthesis of unsaturated fatty acids, PPAR signaling pathway
• MW: 49.8 kDa
• Gene Summary: The protein encoded by this gene is a member of the fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family members are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase portion, both of which are characterized by conserved histidine motifs. This gene is clustered with family members at 11q12-q13.1; this cluster is thought to have arisen evolutionarily from gene duplication based on its similar exon/intron organization. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]