SHANK3 (NM_033517) Human Tagged ORF Clone

CAT#: RC235332

SHANK3 (Myc-DDK tagged) - Homo sapiens SH3 and multiple ankyrin repeat domains 3 (SHANK3)

Product Images

Circular map for RC235332

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SHANK3
• Synonyms: DEL22q13.3; PROSAP2; PSAP2; SCZD15; SPANK-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC235332 representing NM_033517
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGGCCCCGGGGCCAGCGCCGTGGTCGTGCGCGTCGGCATCCCGGACCTGCAGCAGACGAAGTGCC
  TGCGCCTGGACCCGGCCGCGCCCGTGTGGGCCGCCAAGCAGCGCGTGCTCTGCGCCCTCAACCACAGCCT
  CCAGGACGCGCTCAACTATGGGCTTTTCCAGCCGCCCTCCCGGGGCCGCGCCGGCAAGTTCCTGGATGAG
  GAGCGGCTCCTGCAGGAGTACCCGCCCAACCTGGACACGCCCCTGCCCTACCTGGAGTTTCGATACAAGC
  GGCGAGTTTATGCCCAGAACCTCATCGATGATAAGCAGTTTGCAAAGCTTCACACAAAGGCGAACCTGAA
  GAAGTTCATGGACTACGTCCAGCTGCATAGCACGGACAAGGTGGCACGCCTGTTGGACAAGGGGCTGGAC
  CCCAACTTCCATGACCCTGACTCAGGAGAGTGCCCCCTGAGCCTCGCAGCCCAGCTGGACAACGCCACGG
  ACCTGCTAAAGGTGCTGAAGAATGGTGGTGCCCACCTGGACTTCCGCACTCGCGATGGGCTCACTGCCGT
  GCACTGTGCCACACGCCAGCGGAATGCGGCAGCACTGACGACCCTGCTGGACCTGGGGGCTTCACCTGAC
  TACAAGGACAGCCGCGGCTTGACACCCCTCTACCACAGCGCCCTGGGGGGTGGGGATGCCCTCTGCTGTG
  AGCTGCTTCTCCACGACCACGCTCAGCTGGGGATCACCGACGAGAATGGCTGGCAGGAGATCCACCAGGC
  CTGCCGCTTTGGGCACGTGCAGCATCTGGAGCACCTGCTGTTCTATGGGGCAGACATGGGGGCCCAGAAC
  GCCTCGGGGAACACAGCCCTGCACATCTGTGCCCTCTACAACCAGGAGAGCTGTGCTCGTGTCCTGCTCT
  TCCGTGGAGCTAACAGGGATGTCCGCAACTACAACAGCCAGACAGCCTTCCAGGTGGCCATCATCGCAGG
  GAACTTTGAGCTTGCAGAGGTTATCAAGACCCACAAAGACTCGGATGTTGTACCATTCAGGGAAACCCCC
  AGCTATGCGAAGCGGCGGCGACTGGCTGGCCCCAGTGGCTTGGCATCCCCTCGGCCTCTGCAGCGCTCAG
  CCAGCGATATCAACCTGAAGGGGGAGGCACAGCCAGCAGCTTCTCCTGGACCCTCGCTGAGAAGCCTCCC
  CCACCAGCTGCTGCTCCAGCGGCTGCAAGAGGAGAAAGATCGTGACCGGGATGCCGACCAGGAGAGCAAC
  ATCAGTGGCCCTTTAGCAGGCAGGGCCGGCCAAAGCAAGATCAGCCCGAGCGGGCCCGGCGGCCCCGGCC
  CCGCGCCCGGCCCCGGCCCCGCGCCCCCTGCGCCCCCCGCACCGCCGCCCCGGGGCCCGAAGCGGAAACT
  TTACAGCGCCGTCCCCGGCCGCAAGTTCATCGCCGTGAAGGCGCACAGCCCGCAGGGTGAAGGCGAGATC
  CCGCTGCACCGCGGCGAGGCCGTGAAGGTGCTCAGCATTGGGGAGGGCGGTTTCTGGGAGGGAACCGTGA
  AAGGCCGCACGGGCTGGTTCCCGGCCGACTGCGTGGAGGAAGTGCAGATGAGGCAGCATGACACACGGCC
  TGAAACGCGGGAGGACCGGACGAAGCGGCTCTTTCGGCACTACACAGTGGGCTCCTACGACAGCCTCACC
  TCACACAGCGATTATGTCATTGATGACAAAGTGGCTGTCCTGCAGAAACGGGACCACGAGGGCTTTGGTT
  TTGTGCTCCGGGGAGCCAAAGCAGAGACCCCCATCGAGGAGTTCACGCCCACGCCAGCCTTCCCGGCGCT
  GCAGTATCTCGAGTCGGTGGACGTGGAGGGTGTGGCCTGGAGGGCCGGGCTGCGCACGGGAGACTTCCTC
  ATCGAGGTGAACGGGGTGAACGTGGTGAAGGTCGGACACAAGCAGGTGGTGGCTCTGATTCGCCAGGGTG
  GCAACCGCCTCGTCATGAAGGTTGTGTCTGTGACAAGGAAGCCAGAAGAGGACGGGGCTCGGCGCAGAGC
  CCCACCGCCCCCCAAGAGGGCCCCCAGCACCACACTGACCCTGCGCTCCAAGTCCATGACAGCTGAGCTC
  GAGGAACTTGCCTCCATTCGGAGAAGAAAAGGGGAGAAGCTGGACGAGATGCTGGCAGCCGCCGCAGAGC
  CAACGCTGCGGCCAGACATCGCAGACGCAGACTCCAGAGCCGCCACCGTCAAACAGAGGCCCACCAGTCG
  GAGGATCACACCCGCCGAGATTAGCTCATTGTTTGAACGCCAGGGCCTCCCAGGCCCAGAGAAGCTGCCG
  GGCTCCTTGCGGAAGGGGATTCCACGGACCAAGTCTGTAGGGGAGGACGAGAAGCTGGCGTCCCTGCTGG
  AAGGGCGCTTCCCGCGGAGCACCTCGATGCAAGACCCGGTGCGCGAGGGTCGCGGCATCCCGCCCCCGCC
  GCAGACCGCGCCGCCTCCCCCGCCCGCGCCCTACTACTTCGACTCGGGGCCGCCCCCGGCCTTCTCGCCG
  CCGCCCCCGCCGGGCCGCGCCTACGACACGGTGCGCTCCAGCTTCAAGCCCGGCCTGGAGGCGCGCCTGG
  GCGCGGGCGCTGCCGGCCTGTACGAGCCGGGCGCGGCCCTCGGCCCGCTGCCGTATCCCGAGCGGCAGAA
  GCGCGCGCGCTCCATGATCATCCTGCAGGACTCGGCGCCCGAGTCGGGCGACGCCCCTCGACCCCCGCCC
  GCGGCCACCCCGCCCGAGCGACCCAAGCGCCGGCCGCGGCCGCCCGGCCCCGACAGCCCCTACGCCAACC
  TGGGCGCCTTCAGCGCCAGCCTCTTCGCTCCGTCCAAGCCGCAGCGCCGCAAGAGCCCCCTGGTGAAGCA
  GCTGCAGGTGGAGGACGCGCAGGAGCGCGCGGCCCTGGCCGTGGGCAGCCCCGGTCCCGGCGGCGGCAGC
  TTCGCCCGCGAGCCCTCCCCGACCCACCGCGGTCCGCGCCCGGGTGGCCTCGACTACGGCGCGGGCGATG
  GCCCGGGGCTCGCGTTCGGCGGCCCGGGCCCGGCCAAGGACCGGCGGCTGGAGGAGCGGCGCCGCTCCAC
  TGTGTTCCTGTCCGTGGGGGCCATCGAGGGCAGCGCCCCCGGCGCGGATCTGCCATCCCTACAGCCCTCC
  CGCTCCATCGACGAGCGCCTCCTGGGGACCGGCCCCACCGCCGGCCGCGACCTGCTGCTGCCCTCCCCGG
  TGTCTGCCCTGAAGCCGTTGGTCAGCGGCCCGAGCCTGGGGCCCTCGGGTTCCACCTTCATCCACCCACT
  CACCGGCAAACCCCTGGACCCCAGCTCACCCCTGGCCCTTGCCCTGGCTGCCCGAGAGCGAGCTCTGGCC
  TCCCAGGCGCCCTCCCGGTCCCCCACACCCGTGCACAGTCCCGACGCCGACCGCCCCGGACCCCTGTTTG
  TGGATGTACAGGCCCGGGACCCAGAGCGAGGGTCCCTGGCTTCCCCGGCTTTCTCCCCACGGAGCCCAGC
  CTGGATTCCTGTGCCTGCTCGCAGGGAGGCAGAGAAGGTCCCCCGGGAGGAGCGGAAGTCACCCGAGGAC
  AAGAAGTCCATGATCCTCAGCGTCCTGGACACATCCCTGCAGCGGCCAGCTGGCCTCATCGTTGTGCACG
  CCACCAGCAACGGGCAGGAGCCCAGCAGGCTGGGGGGGGCCGAAGAGGAGCGCCCGGGCACCCCGGAGTT
  GGCCCCGGCCCCCATGCAGTCAGCGGCTGTGGCAGAGCCCCTGCCCAGCCCCCGGGCCCAGCCCCCTGGT
  GGCACCCCGGCAGACGCCGGGCCAGGCCAGGGCAGCTCAGAGGAAGAGCCAGAGCTGGTGTTTGCTGTGA
  ACCTGCCACCTGCCCAGCTGTCGTCCAGCGATGAGGAGACCAGGGAGGAGCTGGCCCGAATTGGGTTGGT
  GCCACCCCCTGAAGAGTTTGCCAACGGGGTCCTGCTGGCCACCCCACTCGCTGGCCCGGGCCCCTCGCCC
  ACCACGGTGCCCAGCCCGGCCTCAGGGAAGCCCAGCAGTGAGCCACCCCCTGCCCCTGAGTCTGCAGCCG
  ACTCTGGGGTGGAGGAGGCTGACACACGCAGCTCCAGCGACCCCCACCTGGAGACCACAAGCACCATCTC
  CACGGTGTCCAGCATGTCCACCTTGAGCTCGGAGAGCGGGGAACTCACTGACACCCACACCTCCTTCGCT
  GACGGACACACTTTTCTACTCGAGAAGCCACCAGTGCCTCCCAAGCCCAAGCTCAAGTCCCCGCTGGGGA
  AGGGGCCGGTGACCTTCAGGGACCCGCTGCTGAAGCAGTCCTCGGACAGCGAGCTCATGGCCCAGCAGCA
  CCACGCCGCCTCTGCCGGGCTGGCCTCTGCCGCCGGGCCTGCCCGCCCTCGCTACCTCTTCCAGAGAAGG
  TCCAAGCTATGGGGGGACCCCGTGGAGAGCCGGGGGCTCCCTGGGCCTGAAGACGACAAACCAACTGTGA
  TCAGTGAGCTCAGCTCCCGCCTGCAGCAGCTGAACAAGGACACGCGTTCCCTGGGGGAGGAACCAGTTGG
  TGGCCTGGGCAGCCTGCTGGACCCTGCCAAGAAGTCGCCCATCGCAGCAGCTCGGCTCTTCAGCAGCCTC
  GGTGAGCTGAGCTCCATTTCAGCGCAGCGCAGCCCCGGGGGCCCGGGCGGCGGGGCCTCGTACTCGGTGA
  GGCCCAGTGGCCGCTACCCCGTGGCGAGACGCGCCCCGAGCCCGGTGAAGCCCGCGTCGCTGGAGCGGGT
  GGAGGGGCTGGGGGCGGGCGCGGGGGGCGCAGGGCGGCCCTTCGGCCTCACGCCCCCCACCATCCTCAAG
  TCGTCCAGCCTCTCCATCCCGCACGAGCCCAAGGAGGTGCGCTTCGTGGTGCGCAGCGTGAGCGCGCGCA
  GTCGCTCCCCCTCGCCGTCGCCGCTGCCCTCGCCCGCGTCCGGCCCCGGCCCCGGCGCCCCCGGCCCACG
  CCGACCCTTCCAGCAGAAGCCGCTGCAGCTCTGGAGCAAGTTCGACGTGGGCGACTGGCTGGAGAGCATC
  CACCTAGGCGAGCACCGCGACCGCTTCGAGGACCATGAGATAGAAGGCGCGCACCTACCCGCGCTTACCA
  AGGACGACTTCGTGGAGCTGGGCGTCACGCGCGTGGGCCACCGCATGAACATCGAGCGCGCGCTCAGGCA
  GCTGGACGGCAGC
  
  GCTAGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATG
  ATATCCTGGATT
  Protein Sequence

  >RC235332 representing NM_033517
  Red=Cloning site Green=Tags(s)
  
  MDGPGASAVVVRVGIPDLQQTKCLRLDPAAPVWAAKQRVLCALNHSLQDALNYGLFQPPSRGRAGKFLDE
  ERLLQEYPPNLDTPLPYLEFRYKRRVYAQNLIDDKQFAKLHTKANLKKFMDYVQLHSTDKVARLLDKGLD
  PNFHDPDSGECPLSLAAQLDNATDLLKVLKNGGAHLDFRTRDGLTAVHCATRQRNAAALTTLLDLGASPD
  YKDSRGLTPLYHSALGGGDALCCELLLHDHAQLGITDENGWQEIHQACRFGHVQHLEHLLFYGADMGAQN
  ASGNTALHICALYNQESCARVLLFRGANRDVRNYNSQTAFQVAIIAGNFELAEVIKTHKDSDVVPFRETP
  SYAKRRRLAGPSGLASPRPLQRSASDINLKGEAQPAASPGPSLRSLPHQLLLQRLQEEKDRDRDADQESN
  ISGPLAGRAGQSKISPSGPGGPGPAPGPGPAPPAPPAPPPRGPKRKLYSAVPGRKFIAVKAHSPQGEGEI
  PLHRGEAVKVLSIGEGGFWEGTVKGRTGWFPADCVEEVQMRQHDTRPETREDRTKRLFRHYTVGSYDSLT
  SHSDYVIDDKVAVLQKRDHEGFGFVLRGAKAETPIEEFTPTPAFPALQYLESVDVEGVAWRAGLRTGDFL
  IEVNGVNVVKVGHKQVVALIRQGGNRLVMKVVSVTRKPEEDGARRRAPPPPKRAPSTTLTLRSKSMTAEL
  EELASIRRRKGEKLDEMLAAAAEPTLRPDIADADSRAATVKQRPTSRRITPAEISSLFERQGLPGPEKLP
  GSLRKGIPRTKSVGEDEKLASLLEGRFPRSTSMQDPVREGRGIPPPPQTAPPPPPAPYYFDSGPPPAFSP
  PPPPGRAYDTVRSSFKPGLEARLGAGAAGLYEPGAALGPLPYPERQKRARSMIILQDSAPESGDAPRPPP
  AATPPERPKRRPRPPGPDSPYANLGAFSASLFAPSKPQRRKSPLVKQLQVEDAQERAALAVGSPGPGGGS
  FAREPSPTHRGPRPGGLDYGAGDGPGLAFGGPGPAKDRRLEERRRSTVFLSVGAIEGSAPGADLPSLQPS
  RSIDERLLGTGPTAGRDLLLPSPVSALKPLVSGPSLGPSGSTFIHPLTGKPLDPSSPLALALAARERALA
  SQAPSRSPTPVHSPDADRPGPLFVDVQARDPERGSLASPAFSPRSPAWIPVPARREAEKVPREERKSPED
  KKSMILSVLDTSLQRPAGLIVVHATSNGQEPSRLGGAEEERPGTPELAPAPMQSAAVAEPLPSPRAQPPG
  GTPADAGPGQGSSEEEPELVFAVNLPPAQLSSSDEETREELARIGLVPPPEEFANGVLLATPLAGPGPSP
  TTVPSPASGKPSSEPPPAPESAADSGVEEADTRSSSDPHLETTSTISTVSSMSTLSSESGELTDTHTSFA
  DGHTFLLEKPPVPPKPKLKSPLGKGPVTFRDPLLKQSSDSELMAQQHHAASAGLASAAGPARPRYLFQRR
  SKLWGDPVESRGLPGPEDDKPTVISELSSRLQQLNKDTRSLGEEPVGGLGSLLDPAKKSPIAAARLFSSL
  GELSSISAQRSPGGPGGGASYSVRPSGRYPVARRAPSPVKPASLERVEGLGAGAGGAGRPFGLTPPTILK
  SSSLSIPHEPKEVRFVVRSVSARSRSPSPSPLPSPASGPGPGAPGPRRPFQQKPLQLWSKFDVGDWLESI
  HLGEHRDRFEDHEIEGAHLPALTKDDFVELGVTRVGHRMNIERALRQLDGS
  
  
• Restriction Sites: SgfI-NheI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_033517
• ORF Size: 5193 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033517.1, NP_277052.1
• RefSeq Size: 7113 bp
• RefSeq ORF: 5196 bp
• Locus ID: 85358
• Cytogenetics: 22q13.33
• MW: 185.1 kDa
• Gene Summary: This gene is a member of the Shank gene family. Shank proteins are multidomain scaffold proteins of the postsynaptic density that connect neurotransmitter receptors, ion channels, and other membrane proteins to the actin cytoskeleton and G-protein-coupled signaling pathways. Shank proteins also play a role in synapse formation and dendritic spine maturation. Mutations in this gene are a cause of autism spectrum disorder (ASD), which is characterized by impairments in social interaction and communication, and restricted behavioral patterns and interests. Mutations in this gene also cause schizophrenia type 15, and are a major causative factor in the neurological symptoms of 22q13.3 deletion syndrome, which is also known as Phelan-McDermid syndrome. Additional isoforms have been described for this gene but they have not yet been experimentally verified. [provided by RefSeq, Mar 2012]