FBXW10 (NM_001267585) Human Tagged ORF Clone

CAT#: RC235141

FBXW10 (Myc-DDK tagged) - Homo sapiens F-box and WD repeat domain containing 10 (FBXW10), transcript variant 1

Product Images

Circular map for RC235141

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FBXW10
• Synonyms: Fbw10; HREP; SM2SH2; SM25H2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC235141 representing NM_001267585
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAAACCTGGAATCAAGGCTCAAGAATGCCCCCTATTTTCGTTGTGAGAAGGGAACCGATTCCATCC
  CTCTATGCCGGAAGTGTGAGACGTGTGTCTTAGCCTGGAAGATCTTCTCTACCAAAGAGTGGTTCTGCAG
  GATCAATGACATATCACAGAGGAGGTTTCTAGTTGGCATTCTGAAGCAGTTAAATAGCTTATATTTGTTA
  CACTATTTCCAAAATATCCTTCAGACCACACAGGGAAAGGATTTCATCTATAACAGGTCCCGGATCAACC
  TCAGCAAGAAAGAGGGGAAAGTTGTGAAGTCCTCCTTGAACCAAATGTTGGATAAAACAGTAGAACAGAA
  GATGAAAGAGATCTTGTACTGGTTTGCGAACAGCACCCAGTGGACCAAGGCGAATTATACTCTCTTACTG
  CTGCAGATGTGCAACCCCAAATTACTGCTCACTGCTGCCAATGTGATCAGAGTCCTGTTTCTGAGAGAGG
  AGAACAATATCTCAGGGCTCAATCAAGACATCACAGATGTGTGTTTTTCCCCTGAGAAAGACCACAGCTC
  CAAGTCTGCGACCTCACAAGTCTATTGGACAGCCAAAACTCAGCACACATCCCTTCCTTTGTCCAAAGCC
  CCAGAAAATGAACACTTGCTTGGGGCAGCATCTAACCCTGAGGAACCATGGAGGAATTCACTCCGGTGTA
  TATCCGAAATGAATAGGCTGTTTTCTGGAAAAGGAGACATAACCAAGCCAGGGTACGATCCCTGCAATCT
  ATTGGTTGACCTGGATGACATCAGAGACCTGTCTTCTGGGTTCAGCAAATACCGAGACTTCATCCGTTAC
  CTGCCCATCCACCTCTCCAAGTACATTCTAAGAATGCTGGATAGACACACCCTGAACAAGTGCGCCTCTG
  TGAGCCAGCACTGGGCCGCCATGGCTCAACAGGTCAAGATGGACTTGTCAGCGCACGGCTTCATTCAGAA
  CCAGATTACCTTCTTGCAGGGGTCCTACACAAGAGGAATTGATCCTAATTATGCCAATAAGGTTTCTATC
  CCAGTTCCTAAAATGGTAGATGACGGGAAGAGCATGCGTGTGAAACATCCGAAGTGGAAGCTGAGAACGA
  AGAATGAGTACAACCTGTGGACTGCATACCAGAACGAGGAAACGCAGCAGGTCCTGATAGAGGAGAGAAA
  TGTTTTCTGTGGGACCTACAATGTTCGCATTCTCTCTGACACGTGGGATCAAAACCGAGTCATCCACTAT
  TCCGGGGGAGATCTGATAGCTGTGTCATCTAATCGAAAGATCCATCTTCTGGACATCATACAAGTGAAAG
  CGATACCCGTTGAATTCCGAGGCCATGCTGGGAGTGTCCGGGCCCTCTTCCTGTGTGAGGAGGAAAACTT
  TCTCCTAAGCGGGAGCTATGACCTAAGTATCAGATACTGGGATCTGAAAAGTGGGGTTTGCACACGAATC
  TTCGGTGGTCACCAGGGGACTATCACTTGCATGGACTTGTGTAAGAACAGGCTCGTATCTGGAGGAAGAG
  ATTGCCAGGTAAAAGTATGGGATGTAGACACAGGGAAGTGCCTGAAGACGTTTAGACACAAAGACCCCAT
  CTTGGCCACCAGGATCAATGATACCTACATTGTGAGCAGCTGTGAGCGAGGGCTGGTGAAAGTGTGGCAC
  ATTGCCATGGCCCAGTTGGTAAAGACTCTCAGTGGCCATGAGGGAGCCGTGAAATGCCTGTTCTTTGACC
  AGTGGCATCTCCTCTCAGGAAGTACTGATGGCCTGGTCATGGCCTGGAGCATGGTGGGGAAGTACGAGCG
  CTGCCTGATGGCCTTCAAGCATCCCAAGGAGGTGCTCGACGTGTCCCTTCTCTTCCTCCGGGTCATCAGC
  GCCTGTGCAGATGGCAAGATCCGAATTTACAATTTCCTCAACGGGAACTGTATGAAGGTGTTAAAAGCCA
  ATGGCAGAGGTGATCCTGTGCTGTCCTTCTTTATTCAGGGCAACAGGATGGTGGTCAACACAGAGAGCAA
  TGTTCTCATGTTCCAGTTTGAGCACATAAAGTGGCAGTATGCCGTGGAAAAAACGAAACAAAAGAAGAAT
  AAGGAGAAAGAGGAGGAAAAAGAAGAAAATAGTCTCATGGAAATTCTCTCTAAGTGTAATATTCAGGTTC
  ACAGCCCAAGAGAGTCTGTATCCAGTAAACAAACTGTGATCCAAGAGCTCCTACCAGGCAAACCTCCCAA
  GTCCCGAGTACTCCTGAAGCCGGCCAAGTTCTCTTCAGCAGTGTTAATAGAGGAACTTCAAAGTCAAGGA
  AAGTCAAAATCACCCCGAAGAGATGCAGATGATGTGGAGAAAGCACAAAAACAAGGACAATTGGAAACTC
  CTGGAAAACTGCCCAGTCACCCAAAGAAAAAGTCTTGGAAAATCCCTATGTCACCTGACCAATTCCTCCT
  GACTGTTAGCGCCCTGCAGCACGCCCATAATTCCGGGGAATTTGCCTATCCCTGTAGGCCCCAAACAGAA
  ATTACTGATGTCTGGGGACCTTCAATTTCATACCCAAGGAAGGTCTTGAATTTCAAAGGAAAATCAATCC
  AACGTGCAGTTGATCGGTTGAGATTGAGCAATCCTCCTATAGATGTGAAACGAACCAGTATTCCCCTTGA
  AATCCAGAAACTGCAGCCCAACTTGAAGATCTCTTTGCACAGTCCTAGAGTCCAGTCCACCATACCCCAG
  CCCATGATTATCCGCTCCAGGTTCTCTGGCAGCTTAAAGGGTGGAGACCAAGTGACCAGTTCAATTGAAA
  GGGCTGTGTGCAGTACGGGTCCCCTGACCAGTATGCAGGTCATTAAACCAAACCGCATGCTAGCTCCACA
  AGTGGGCACAGCCACCCTGTCTCTTAAGAAAGAACGGCCTCGCATCTATACAGCCCTTGATCCTTTTAGA
  GTGAACACTGAGTTCGTGCTGTTGACCGTGAAGGAGGAGAAGGAGCACCAGGAAGCCAAGATGAAGGAAT
  ATCAGGCCAGGGAGTCCACTGGAGTGGTTGATCCAGGAAAAGTCAGCAAAGCTGCATGGATCAGGAAGAT
  CAAAGGCCTGCCTATTGATAATTTCACGAAGCAAGGGAAAACAGCGGCCCCTGAACTTGGACAAAATGTA
  TTTATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC235141 representing NM_001267585
  Red=Cloning site Green=Tags(s)
  
  MENLESRLKNAPYFRCEKGTDSIPLCRKCETCVLAWKIFSTKEWFCRINDISQRRFLVGILKQLNSLYLL
  HYFQNILQTTQGKDFIYNRSRINLSKKEGKVVKSSLNQMLDKTVEQKMKEILYWFANSTQWTKANYTLLL
  LQMCNPKLLLTAANVIRVLFLREENNISGLNQDITDVCFSPEKDHSSKSATSQVYWTAKTQHTSLPLSKA
  PENEHLLGAASNPEEPWRNSLRCISEMNRLFSGKGDITKPGYDPCNLLVDLDDIRDLSSGFSKYRDFIRY
  LPIHLSKYILRMLDRHTLNKCASVSQHWAAMAQQVKMDLSAHGFIQNQITFLQGSYTRGIDPNYANKVSI
  PVPKMVDDGKSMRVKHPKWKLRTKNEYNLWTAYQNEETQQVLIEERNVFCGTYNVRILSDTWDQNRVIHY
  SGGDLIAVSSNRKIHLLDIIQVKAIPVEFRGHAGSVRALFLCEEENFLLSGSYDLSIRYWDLKSGVCTRI
  FGGHQGTITCMDLCKNRLVSGGRDCQVKVWDVDTGKCLKTFRHKDPILATRINDTYIVSSCERGLVKVWH
  IAMAQLVKTLSGHEGAVKCLFFDQWHLLSGSTDGLVMAWSMVGKYERCLMAFKHPKEVLDVSLLFLRVIS
  ACADGKIRIYNFLNGNCMKVLKANGRGDPVLSFFIQGNRMVVNTESNVLMFQFEHIKWQYAVEKTKQKKN
  KEKEEEKEENSLMEILSKCNIQVHSPRESVSSKQTVIQELLPGKPPKSRVLLKPAKFSSAVLIEELQSQG
  KSKSPRRDADDVEKAQKQGQLETPGKLPSHPKKKSWKIPMSPDQFLLTVSALQHAHNSGEFAYPCRPQTE
  ITDVWGPSISYPRKVLNFKGKSIQRAVDRLRLSNPPIDVKRTSIPLEIQKLQPNLKISLHSPRVQSTIPQ
  PMIIRSRFSGSLKGGDQVTSSIERAVCSTGPLTSMQVIKPNRMLAPQVGTATLSLKKERPRIYTALDPFR
  VNTEFVLLTVKEEKEHQEAKMKEYQARESTGVVDPGKVSKAAWIRKIKGLPIDNFTKQGKTAAPELGQNV
  FI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001267585
• ORF Size: 3156 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001267585.2
• RefSeq Size: 3452 bp
• RefSeq ORF: 3159 bp
• Locus ID: 10517
• UniProt ID: Q5XX13
• Cytogenetics: 17p11.2
• MW: 120.3 kDa
• Gene Summary: Members of the F-box protein family, such as FBXW10, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM, Mar 2008]