GTF2IRD1 (NM_001199207) Human Tagged ORF Clone

CAT#: RC235096

GTF2IRD1 (Myc-DDK tagged) - Homo sapiens GTF2I repeat domain containing 1 (GTF2IRD1), transcript variant 3

Product Images

Circular map for RC235096

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GTF2IRD1
• Synonyms: BEN; CREAM1; GTF3; hMusTRD1alpha1; MUSTRD1; RBAP2; WBS; WBSCR11; WBSCR12
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC235096 representing NM_001199207
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTTGCTGGGTAAGCGCTGTGACGTCCCCACCAACGGCTGCGGACCCGACCGCTGGAACTCCGCGT
  TCACCCGCAAAGACGAGATCATCACCAGCCTCGTGTCTGCCTTAGACTCCATGTGCTCAGCGCTGTCCAA
  ACTGAACGCCGAGGTGGCCTGTGTCGCCGTGCACGATGAGAGCGCCTTTGTGGTGGGCACAGAGAAGGGG
  AGAATGTTCCTGAATGCCCGGAAGGAGCTACAGTCAGACTTCCTCAGGTTCTGCCTCTCCGCAGCTCAGC
  ACAGGGCAGCGACATCCCAGCTCGAAGGCCGGGTGGTGAGACGGGTGCTCACTGTGGCCTCGCGTGCTCT
  GTGTCCCACAGGAGGGCCCCCGTGGAAGGATCCGGAGGCAGAGCACCCCAAGAAGGTGCAGCGGGGCGAG
  GGTGGAGGCCGTAGCCTCCCTCGGTCCTCCCTGGAACATGGCTCAGATGTGTACCTTCTGCGGAAGATGG
  TAGAGGAGGTGTTTGATGTTCTTTATAGCGAGGCCCTGGGAAGGGCCAGTGTGGTGCCACTGCCCTATGA
  GAGGCTGCTCAGGGAGCCAGGGCTGCTGGCCGTGCAGGGGCTGCCCGAAGGCCTGGCCTTCCGAAGGCCA
  GCCGAGTATGACCCCAAGGCCCTCATGGCCATCCTGGAACACAGCCACCGCATCCGCTTCAAGCTCAAGA
  GGCCACTTGAGGATGGCGGGCGGGACTCGAAGGCCCTGGTGGAGCTGAACGGTGTCTCCCTGATTCCCAA
  GGGGTCACGGGACTGTGGCCTGCATGGCCAGGCCCCCAAGGTGCCACCCCAGGACCTGCCCCCAACCGCC
  ACCTCCTCCTCCATGGCCAGCTTCCTGTACAGCACGGCGCTCCCCAACCACGCCATCCGAGAGCTCAAGC
  AGGAAGCACCTTCCTGCCCCCTTGCCCCCAGCGACCTGGGCCTGAGTCGGCCCATGCCAGAGCCCAAGGC
  CACCGGTGCCCAAGACTTCTCCGACTGTTGTGGACAGAAGCCCACTGGGCCTGGTGGGCCTCTCATCCAG
  AACGTCCATGCCTCCAAGCGCATTCTCTTCTCCATCGTCCATGACAAGTCAGAGAAGTGGGACGCCTTCA
  TAAAGGAAACCGAGGACATCAACACGCTCCGGGAGTGTGTGCAGATCCTGTTTAACAGCAGATATGCGGA
  AGCCCTGGGCCTGGACCACATGGTCCCCGTGCCCTACCGGAAGATTGCCTGTGACCCGGAGGCTGTGGAG
  ATCGTGGGCATCCCGGACAAGATCCCCTTCAAGCGCCCCTGCACTTATGGAGTCCCCAAGCTGAAGCGGA
  TCCTGGAGGAGCGCCATAGTATCCACTTCATCATTAAGAGGATGTTTGATGAGCGAATTTTCACAGGGAA
  CAAGTTTACCAAAGACACCACGAAGCTGGAGCCAGCCAGCCCGCCAGAGGACACCTCTGCAGAGGTCTCT
  AGGGCCACCGTCCTTGACCTTGCTGGGAATGCTCGGTCAGACAAGGGCAGCATGTCTGAAGACTGTGGGC
  CAGGAACCTCCGGGGAGCTGGGCGGGCTGAGGCCGATCAAAATTGAGCCAGAGGATCTGGACATCATTCA
  GGTCACCGTCCCAGACCCCTCGCCAACCTCTGAGGAAATGACAGACTCGATGCCTGGGCACCTGCCATCG
  GAGGATTCTGGTTATGGGATGGAGATGCTGACAGACAAAGGTCTGAGTGAGGACGCGCGGCCCGAGGAGA
  GGCCCGTGGAGGACAGCCACGGTGACGTGATCCGGCCCCTGCGGAAGCAGGTGGAGCTGCTCTTCAACAC
  ACGATACGCCAAGGCCATTGGCATCTCGGAGCCCGTCAAGGTGCCGTACTCCAAGTTTCTGATGCACCCG
  GAGGAGCTGTTTGTGGTGGGACTGCCTGAAGGCATCTCCCTCCGCAGGCCCAACTGCTTCGGGATCGCCA
  AGCTCCGGAAGATTCTGGAGGCCAGCAACAGCATCCAGTTTGTCATCAAGAGGCCCGAGCTGCTCACTGA
  GGGAGTCAAAGAGCCCATCATGGATAGTCAAGAGAGGGATTCCGGGGACCCTCTGGTGGACGAGAGCCTG
  AAGAGACAGGGCTTTCAAGAAAATTATGACGCGAGGCTCTCACGGATCGACATCGCCAACACACTAAGGG
  AGCAGGTCCAGGACCTTTTCAATAAGAAATACGGGGAAGCCTTGGGCATCAAGTACCCGGTCCAGGTCCC
  CTACAAGCGGATCAAGAGTAACCCCGGCTCCGTGATCATCGAGGGGCTGCCCCCAGGAATCCCGTTCCGA
  AAGCCCTGTACCTTCGGCTCCCAGAACCTGGAGAGGATTCTTGCTGTGGCTGACAAGATCAAGTTCACAG
  TCACCAGGCCTTTCCAAGGACTCATCCCAAAGCCTGATGAAGATGACGCCAACAGACTCGGGGAGAAGGT
  GATCCTGCGGGAGCAGGTGAAGGAACTCTTCAACGAGAAATACGGTGAGGCCCTGGGCCTGAACCGGCCG
  GTGCTGGTCCCTTATAAACTAATCCGGGACAGCCCAGACGCCGTGGAGGTCACGGGTCTGCCTGATGACA
  TCCCCTTCCGGAACCCCAACACGTACGACATCCACCGGCTGGAGAAGATCCTGAAGGCCCGAGAGCATGT
  CCGCATGGTCATCATTAACCAGCTCCAACCCTTTGCAGAAATCTGCAATGATGCCAAGGTGCCAGCCAAA
  GACAGCAGCATTCCCAAGCGCAAGAGAAAGCGGGTCTCGGAAGGAAATTCCGTCTCCTCTTCCTCCTCGT
  CTTCCTCTTCCTCGTCCTCTAACCCGGATTCAGTGGCATCGGCCAACCAGATCTCACTCGTGCAATGGCC
  AATGTACATGGTGGACTATGCCGGCCTGAACGTGCAGCTCCCGGGACCTCTTAATTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC235096 representing NM_001199207
  Red=Cloning site Green=Tags(s)
  
  MALLGKRCDVPTNGCGPDRWNSAFTRKDEIITSLVSALDSMCSALSKLNAEVACVAVHDESAFVVGTEKG
  RMFLNARKELQSDFLRFCLSAAQHRAATSQLEGRVVRRVLTVASRALCPTGGPPWKDPEAEHPKKVQRGE
  GGGRSLPRSSLEHGSDVYLLRKMVEEVFDVLYSEALGRASVVPLPYERLLREPGLLAVQGLPEGLAFRRP
  AEYDPKALMAILEHSHRIRFKLKRPLEDGGRDSKALVELNGVSLIPKGSRDCGLHGQAPKVPPQDLPPTA
  TSSSMASFLYSTALPNHAIRELKQEAPSCPLAPSDLGLSRPMPEPKATGAQDFSDCCGQKPTGPGGPLIQ
  NVHASKRILFSIVHDKSEKWDAFIKETEDINTLRECVQILFNSRYAEALGLDHMVPVPYRKIACDPEAVE
  IVGIPDKIPFKRPCTYGVPKLKRILEERHSIHFIIKRMFDERIFTGNKFTKDTTKLEPASPPEDTSAEVS
  RATVLDLAGNARSDKGSMSEDCGPGTSGELGGLRPIKIEPEDLDIIQVTVPDPSPTSEEMTDSMPGHLPS
  EDSGYGMEMLTDKGLSEDARPEERPVEDSHGDVIRPLRKQVELLFNTRYAKAIGISEPVKVPYSKFLMHP
  EELFVVGLPEGISLRRPNCFGIAKLRKILEASNSIQFVIKRPELLTEGVKEPIMDSQERDSGDPLVDESL
  KRQGFQENYDARLSRIDIANTLREQVQDLFNKKYGEALGIKYPVQVPYKRIKSNPGSVIIEGLPPGIPFR
  KPCTFGSQNLERILAVADKIKFTVTRPFQGLIPKPDEDDANRLGEKVILREQVKELFNEKYGEALGLNRP
  VLVPYKLIRDSPDAVEVTGLPDDIPFRNPNTYDIHRLEKILKAREHVRMVIINQLQPFAEICNDAKVPAK
  DSSIPKRKRKRVSEGNSVSSSSSSSSSSSSNPDSVASANQISLVQWPMYMVDYAGLNVQLPGPLNY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001199207
• ORF Size: 2928 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001199207.2
• RefSeq Size: 3522 bp
• RefSeq ORF: 2931 bp
• Locus ID: 9569
• UniProt ID: Q9UHL9
• Cytogenetics: 7q11.23
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Basal transcription factors
• MW: 108.4 kDa
• Gene Summary: The protein encoded by this gene contains five GTF2I-like repeats and each repeat possesses a potential helix-loop-helix (HLH) motif. It may have the ability to interact with other HLH-proteins and function as a transcription factor or as a positive transcriptional regulator under the control of Retinoblastoma protein. This gene plays a role in craniofacial and cognitive development and mutations have been associated with Williams-Beuren syndrome, a multisystem developmental disorder caused by deletion of multiple genes at 7q11.23. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2010]