SLC14A2 (NM_001242692) Human Tagged ORF Clone

CAT#: RC235035

SLC14A2 (Myc-DDK tagged) - Homo sapiens solute carrier family 14 (urea transporter), member 2 (SLC14A2), transcript variant 2

Product Images

Circular map for RC235035

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SLC14A2
• Synonyms: hUT-A6; HUT2; UT-A2; UT2; UTA; UTR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC235035 representing NM_001242692
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGACCCCCACAGCAGTCCTCTCCTGCCAGAGCCACTTTCCAGCAGATACAAACTCTACGAGGCAG
  AGTTTACCAGCCCGAGCTGGCCCTCGACATCCCCGGATACTCACCCAGCTCTGCCCCTCCTGGAAATGCC
  TGAAGAAAAGGATCTCCGGTCTTCCAATGAAGACAGTCACATTGTGAAGATCGAAAAGCTCAATGAAAGG
  AGTAAAAGGAAAGACGACGGGGTGGCCCATCGGGACTCAGCAGGCCAAAGGTGCATCTGCCTCTCCAAAG
  CAGTGGGCTACCTCACGGGCGACATGAAGGAGTACAGGATCTGGCTGAAAGACAAGCACCTTGCCCTCCA
  GTTCATAGACTGGGTCCTGAGAGGGACCGCTCAGGTGATGTTCATCAACAATCCTCTCAGCGGCCTCATC
  ATCTTCATAGGGCTGCTGATCCAGAATCCCTGGTGGACAATCACTGGGGGCCTGGGGACAGTGGTCTCGA
  CCTTAACAGCTCTCGCCTTGGGCCAAGACAGGTCTGCCATTGCCTCAGGACTCCATGGGTACAACGGGAT
  GCTGGTGGGACTGCTGATGGCCGTGTTCTCGGAGAAGTTAGACTACTACTGGTGGCTTCTGTTTCCTGTG
  ACCTTCACAGCCATGTCCTGCCCAGTTCTTTCTAGTGCCTTGAATTCCATCTTCAGCAAGTGGGACCTCC
  CGGTCTTCACTCTGCCCTTCAACATTGCAGTCACCTTGTACCTTGCAGCCACAGGCCACTACAACCTCTT
  CTTCCCCACAACACTGGTAGAGCCTGTGTCTTCAGTGCCCAATATCACCTGGACAGAGATGGAAATGCCC
  CTGCTGTTACAAGCCATCCCTGTTGGGGTCGGCCAGGTGTATGGCTGTGACAATCCCTGGACAGGCGGCG
  TGTTCCTGGTGGCTCTGTTCATCTCCTCGCCACTCATCTGCTTGCATGCAGCCATTGGCTCAATCGTGGG
  GCTGCTAGCAGCCCTGTCAGTGGCCACACCCTTCGAGACCATCTACACAGGCCTCTGGAGCTACAACTGC
  GTCCTCTCCTGCATCGCCATCGGAGGCATGTTCTATGCCCTCACCTGGCAGACTCACCTGCTGGCCCTCA
  TCTGTGCCCTGTTCTGTGCATACATGGAAGCAGCCATCTCCAACATCATGTCAGTGGTGGGCGTGCCACC
  AGGCACCTGGGCCTTCTGCCTTGCCACCATCATCTTCCTGCTCCTGACGACAAACAACCCAGCCATCTTC
  AGACTCCCACTCAGCAAAGTCACCTACCCCGAGGCCAACCGCATCTACTACCTGACAGTGAAAAGCGGTG
  AAGAAGAGAAGGCCCCCAGCGGAGGCGGTGGGGAGCATCCACCCACAGCAGGCCCAAAGGTGGAGGAGGG
  CTCGGAGGCTGTGCTCTCCAAGCACAGGAGTGTATTTCACATCGAGTGGTCATCCATTCGGAGGAGGAGC
  AAAGTGTTTGGAAAAGGCGAACACCAGGAAAGACAAAACAAAGACCCATTTCCCTATCGATACCGGAAGC
  CCACAGTCGAGCTGCTTGATCTGGACACCATGGAGGAGAGCTCTGAGATAAAAGTGGAAACAAACATTTC
  CAAGACATCCTGGATTCGGAGTTCCATGGCTGCCAGTGGGAAAAGGGTCAGCAAAGCCCTCAGCTACATC
  ACAGGAGAGATGAAGGAGTGTGGAGAGGGACTTAAAGACAAGTCCCCAGTGTTCCAGTTCTTTGACTGGG
  TCCTCCGAGGCACATCTCAAGTGATGTTTGTGAACAACCCCCTCAGCGGCATCCTCATCATCCTCGGCCT
  CTTCATCCAGAACCCCTGGTGGGCGATCTCAGGCTGCCTGGGTACCATCATGTCCACCTTGACAGCCCTC
  ATCCTGAGTCAGGACAAGTCGGCCATCGCTGCAGGATTTCACGGCTACAATGGGGTGCTGGTGGGGCTGC
  TGATGGCCGTGTTCTCAGACAAAGGTGACTACTACTGGTGGCTGTTGCTACCCGTCATCATCATGTCCAT
  GTCTTGCCCCATCCTCTCCAGTGCCCTGGGTACCATCTTCAGCAAGTGGGACCTCCCAGTCTTCACACTG
  CCCTTCAATATCACTGTGACTTTGTACCTGGCAGCCACGGGCCACTACAACCTTTTCTTCCCCACAACGC
  TGCTGCAGCCTGCATCCGCCATGCCCAACATCACCTGGTCAGAGGTCCAAGTGCCCTTGCTTTTGAGAGC
  CATCCCCGTTGGAATTGGCCAAGTGTACGGCTGTGATAACCCCTGGACTGGAGGCATCTTCCTCATAGCT
  CTGTTCATATCCTCACCTCTCATTTGCTTGCATGCAGCAATTGGATCCACCATGGGGATGCTAGCAGCAC
  TCACTATTGCGACGCCCTTTGACTCCATCTACTTCGGCCTGTGTGGCTTCAACAGCACCCTCGCATGCAT
  AGCGATAGGAGGCATGTTCTACGTCATCACCTGGCAGACGCACCTCCTCGCCATCGCCTGCGCACTGTTT
  GCTGCCTACCTGGGTGCTGCCCTGGCTAACATGTTATCTGTGTTTGGATTGCCGCCCTGCACTTGGCCCT
  TCTGTCTCTCAGCTCTCACCTTCCTGCTCCTGACGACCAATAACCCCGCCATCTACAAGCTCCCGCTCAG
  CAAAGTCACCTACCCAGAGGCCAACCGCATCTACTACCTGTCCCAGGAGAGAAACAGAAGGGCATCAATC
  ATAACAAAGTATCAGGCCTACGATGTCTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC235035 representing NM_001242692
  Red=Cloning site Green=Tags(s)
  
  MSDPHSSPLLPEPLSSRYKLYEAEFTSPSWPSTSPDTHPALPLLEMPEEKDLRSSNEDSHIVKIEKLNER
  SKRKDDGVAHRDSAGQRCICLSKAVGYLTGDMKEYRIWLKDKHLALQFIDWVLRGTAQVMFINNPLSGLI
  IFIGLLIQNPWWTITGGLGTVVSTLTALALGQDRSAIASGLHGYNGMLVGLLMAVFSEKLDYYWWLLFPV
  TFTAMSCPVLSSALNSIFSKWDLPVFTLPFNIAVTLYLAATGHYNLFFPTTLVEPVSSVPNITWTEMEMP
  LLLQAIPVGVGQVYGCDNPWTGGVFLVALFISSPLICLHAAIGSIVGLLAALSVATPFETIYTGLWSYNC
  VLSCIAIGGMFYALTWQTHLLALICALFCAYMEAAISNIMSVVGVPPGTWAFCLATIIFLLLTTNNPAIF
  RLPLSKVTYPEANRIYYLTVKSGEEEKAPSGGGGEHPPTAGPKVEEGSEAVLSKHRSVFHIEWSSIRRRS
  KVFGKGEHQERQNKDPFPYRYRKPTVELLDLDTMEESSEIKVETNISKTSWIRSSMAASGKRVSKALSYI
  TGEMKECGEGLKDKSPVFQFFDWVLRGTSQVMFVNNPLSGILIILGLFIQNPWWAISGCLGTIMSTLTAL
  ILSQDKSAIAAGFHGYNGVLVGLLMAVFSDKGDYYWWLLLPVIIMSMSCPILSSALGTIFSKWDLPVFTL
  PFNITVTLYLAATGHYNLFFPTTLLQPASAMPNITWSEVQVPLLLRAIPVGIGQVYGCDNPWTGGIFLIA
  LFISSPLICLHAAIGSTMGMLAALTIATPFDSIYFGLCGFNSTLACIAIGGMFYVITWQTHLLAIACALF
  AAYLGAALANMLSVFGLPPCTWPFCLSALTFLLLTTNNPAIYKLPLSKVTYPEANRIYYLSQERNRRASI
  ITKYQAYDVS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001242692
• ORF Size: 2760 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001242692.2
• RefSeq Size: 3685 bp
• RefSeq ORF: 2763 bp
• Locus ID: 8170
• UniProt ID: Q15849
• Cytogenetics: 18q12.3
• Protein Families: Transmembrane
• MW: 101.7 kDa
• Gene Summary: The protein encoded by this gene belongs to the urea transporter family. In mammalian cells, urea is the chief end product of nitrogen catabolism, and plays an important role in the urinary concentration mechanism. This protein is expressed in the inner medulla of the kidney, and mediates rapid transepithelial urea transport across the inner medullary collecting duct. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2011]