ALDH1L1 (NM_001270364) Human Tagged ORF Clone

CAT#: RC235023

ALDH1L1 (Myc-DDK tagged) - Homo sapiens aldehyde dehydrogenase 1 family, member L1 (ALDH1L1), transcript variant 1

Product Images

Circular map for RC235023

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ALDH1L1
• Synonyms: 10-fTHF; 10-FTHFDH; FDH; FTHFD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC235023 representing NM_001270364
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGGTCCTTCCAACCCTCCTGCTACCATGAAGATTGCAGTGATTGGACAGAGCCTGTTTGGCCAGG
  AAGTTTACTGCCACCTGAGGAAGGAGGGCCACGAAGTGGTGGGTGTGTTCACTGTTCCAGACAAGGATGG
  AAAGGCCGACCCCCTGGGTCTGGAAGCTGAGAAGGATGGAGTGCCGGTATTCAAGTACTCCCGGTGGCGT
  GCAAAAGGACAGGCTTTGCCTGATGTGGTGGCAAAATACCAGGCTTTGGGGGCCGAGCTCAACGTCCTGC
  CCTTCTGCAGCCAATTCATCCCCATGGAGATAATCAGTGCCCCCCGGCATGGCTCCATCATCTATCACCC
  GTCACTGCTCCCTAGGCACCGAGGGGCCTCGGCCATCAACTGGACCCTCATTCACGGAGATAAGAAAGGG
  GGGTTTTCCATCTTCTGGGCGGATGATGGTCTGGACACCGGAGACCTGCTGCTGCAGAAGGAGTGTGAGG
  TGCTCCCGGACGACACCGTGAGCACGCTGTACAACCGCTTCCTCTTCCCTGAAGGCATCAAAGGGATGGT
  GCAGGCCGTGAGGCTGATCGCTGAGGGCAAAGCCCCCAGACTCCCTCAGCCTGAGGAAGGAGCCACCTAT
  GAGGGGATTCAGAAGAAGGAGACAGCCAAGATCAACTGGGACCAGCCGGCAGAGGCCATTCACAACTGGA
  TCCGCGGGAACGACAAGGTGCCGGGAGCCTGGACAGAGGCCTGTGAACAGAAACTGACATTTTTCAACTC
  AACGCTGAACACTTCAGGCCTGGTGCCCGAGGGAGACGCTTTGCCCATCCCAGGAGCCCATCGGCCAGGG
  GTGGTCACCAAAGCAGGACTCATCCTCTTTGGGAATGATGACAAAATGCTGCTGGTGAAGAATATTCAGC
  TGGAGGATGGCAAAATGATCCTGGCCTCGAACTTCTTTAAGGGGGCAGCCAGCAGTGTCCTTGAGCTGAC
  AGAGGCAGAGCTGGTTACTGCGGAGGCTGTGCGGAGTGTTTGGCAGCGGATCCTCCCCAAAGTCCTGGAG
  GTTGAAGACTCCACTGATTTCTTCAAGTCAGGGGCCGCGTCTGTGGACGTTGTGAGGCTGGTGGAGGAAG
  TGAAGGAGCTGTGTGATGGCCTGGAGTTAGAAAATGAAGATGTGTACATGGCATCCACCTTTGGGGACTT
  CATCCAGCTGTTAGTGAGGAAGCTGCGAGGGGACGATGAGGAGGGCGAGTGCAGCATTGACTACGTGGAA
  ATGGCAGTGAACAAGCGCACTGTCCGCATGCCCCACCAGCTCTTCATTGGGGGGGAGTTCGTGGATGCCG
  AGGGCGCCAAGACCTCTGAGACCATCAATCCCACCGATGGAAGTGTCATCTGCCAGGTATCCCTGGCCCA
  AGTCACCGACGTCGACAAGGCAGTGGCCGCAGCCAAGGATGCCTTTGAGAATGGACGGTGGGGGAAGATC
  AGTGCGCGGGACCGGGGCCGGCTGATGTACAGGTTGGCAGATCTCATGGAGCAGCACCAGGAGGAGCTGG
  CCACCATTGAGGCCCTGGATGCGGGTGCCGTCTACACGCTGGCCCTGAAGACCCACGTGGGCATGTCCAT
  CCAGACCTTCCGCTACTTTGCTGGCTGGTGTGACAAGATCCAGGGCTCCACCATCCCCATCAACCAGGCC
  AGACCCAACCGCAACCTGACCTTGACCAGGAAGGAGCCTGTTGGGGTTTGTGGCATCATCATCCCCTGGA
  ACTATCCCCTGATGATGCTGTCCTGGAAGACAGCTGCCTGCCTGGCTGCCGGGAACACAGTGGTGATCAA
  GCCTGCTCAGGTGACCCCACTCACAGCCTTGAAGTTTGCAGAGCTGACATTAAAGGCCGGCATTCCCAAA
  GGTGTGGTTAACGTCCTCCCAGGATCTGGCTCCCTGGTCGGCCAGAGACTCTCAGACCATCCTGATGTGA
  GGAAAATCGGGTTCACAGGCTCCACAGAGGTGGGCAAGCACATCATGAAAAGCTGTGCCATAAGTAACGT
  GAAGAAGGTGTCCCTGGAACTGGGCGGGAAGTCACCCCTCATCATCTTTGCTGACTGTGACCTCAACAAG
  GCTGTGCAGATGGGGATGAGTTCTGTTTTCTTCAACAAAGGAGAGAATTGCATTGCAGCAGGCCGACTCT
  TTGTGGAGGACTCCATTCATGATGAGTTCGTGCGGAGAGTGGTAGAAGAGGTGCGGAAGATGAAGGTGGG
  CAACCCGCTGGACAGGGACACCGACCACGGGCCGCAGAATCACCATGCCCACCTTGTGAAGCTGATGGAG
  TACTGCCAGCATGGCGTGAAGGAAGGGGCCACACTGGTCTGCGGCGGGAATCAGGTCCCTCGGCCAGGGT
  TCTTCTTTGAGCCAACTGTTTTCACAGACGTGGAAGACCACATGTTCATAGCCAAGGAGGAGTCCTTCGG
  GCCTGTCATGATCATCTCTCGGTTTGCTGATGGGGACTTGGATGCCGTGCTGTCTCGGGCCAATGCCACG
  GAATTTGGCCTGGCTTCTGGTGTCTTCACCAGGGACATCAACAAGGCCCTGTATGTCAGTGACAAGCTCC
  AGGCAGGCACTGTGTTTGTCAACACGTACAACAAGACCGACGTGGCCGCTCCCTTCGGAGGATTCAAACA
  GTCTGGATTTGGCAAAGATCTAGGAGAGGCGGCTCTGAACGAGTACCTGCGGGTCAAGACAGTGACCTTC
  GAATAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC235023 representing NM_001270364
  Red=Cloning site Green=Tags(s)
  
  MAGPSNPPATMKIAVIGQSLFGQEVYCHLRKEGHEVVGVFTVPDKDGKADPLGLEAEKDGVPVFKYSRWR
  AKGQALPDVVAKYQALGAELNVLPFCSQFIPMEIISAPRHGSIIYHPSLLPRHRGASAINWTLIHGDKKG
  GFSIFWADDGLDTGDLLLQKECEVLPDDTVSTLYNRFLFPEGIKGMVQAVRLIAEGKAPRLPQPEEGATY
  EGIQKKETAKINWDQPAEAIHNWIRGNDKVPGAWTEACEQKLTFFNSTLNTSGLVPEGDALPIPGAHRPG
  VVTKAGLILFGNDDKMLLVKNIQLEDGKMILASNFFKGAASSVLELTEAELVTAEAVRSVWQRILPKVLE
  VEDSTDFFKSGAASVDVVRLVEEVKELCDGLELENEDVYMASTFGDFIQLLVRKLRGDDEEGECSIDYVE
  MAVNKRTVRMPHQLFIGGEFVDAEGAKTSETINPTDGSVICQVSLAQVTDVDKAVAAAKDAFENGRWGKI
  SARDRGRLMYRLADLMEQHQEELATIEALDAGAVYTLALKTHVGMSIQTFRYFAGWCDKIQGSTIPINQA
  RPNRNLTLTRKEPVGVCGIIIPWNYPLMMLSWKTAACLAAGNTVVIKPAQVTPLTALKFAELTLKAGIPK
  GVVNVLPGSGSLVGQRLSDHPDVRKIGFTGSTEVGKHIMKSCAISNVKKVSLELGGKSPLIIFADCDLNK
  AVQMGMSSVFFNKGENCIAAGRLFVEDSIHDEFVRRVVEEVRKMKVGNPLDRDTDHGPQNHHAHLVKLME
  YCQHGVKEGATLVCGGNQVPRPGFFFEPTVFTDVEDHMFIAKEESFGPVMIISRFADGDLDAVLSRANAT
  EFGLASGVFTRDINKALYVSDKLQAGTVFVNTYNKTDVAAPFGGFKQSGFGKDLGEAALNEYLRVKTVTF
  EY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001270364
• ORF Size: 2736 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001270364.1, NP_001257293.1
• RefSeq Size: 3204 bp
• RefSeq ORF: 2739 bp
• Locus ID: 10840
• UniProt ID: O75891
• Cytogenetics: 3q21.3
• Protein Families: Druggable Genome
• Protein Pathways: One carbon pool by folate
• MW: 100.2 kDa
• Gene Summary: The protein encoded by this gene catalyzes the conversion of 10-formyltetrahydrofolate, nicotinamide adenine dinucleotide phosphate (NADP+), and water to tetrahydrofolate, NADPH, and carbon dioxide. The encoded protein belongs to the aldehyde dehydrogenase family. Loss of function or expression of this gene is associated with decreased apoptosis, increased cell motility, and cancer progression. There is an antisense transcript that overlaps on the opposite strand with this gene locus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2012]