PODN (NM_001199081) Human Tagged ORF Clone

CAT#: RC234637

PODN (Myc-DDK tagged) - Homo sapiens podocan (PODN), transcript variant 3

Product Images

Circular map for RC234637

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PODN
• Synonyms: PCAN; SLRR5A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC234637 representing NM_001199081
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGGCGAGGAGGCAGAACAGCCTGCCTGGTTCCGTCAGCCCTGGCGCCCAGGCGCATCTGACTCGG
  CACCCCCTGCAGGCACCATGGCCCAGAGCCGGGTGCTGCTGCTCCTGCTGCTGCTGCCGCCACAGCTGCA
  CCTGGGACCTGTGCTTGCCGTGAGGGCCCCAGGATTTGGCCGAAGTGGCGGCCACAGCCTGAGCCCCGAA
  GAGAACGAATTTGCGGAGGAGGAGCCGGTGCTGGTACTGAGCCCTGAGGAGCCCGGGCCTGGCCCAGCCG
  CGGTCAGCTGCCCCCGAGACTGTGCCTGTTCCCAGGAGGGCGTCGTGGACTGTGGCGGTATTGACCTGCG
  TGAGTTCCCGGGGGACCTGCCTGAGCACACCAACCACCTATCTCTGCAGAACAACCAGCTGGAAAAGATC
  TACCCTGAGGAGCTCTCCCGGCTGCACCGGCTGGAGACGCTGAACCTGCAAAACAACCGCCTGACTTCCC
  GAGGGCTCCCAGAGAAGGCGTTTGAGCATCTGACCAACCTCAATTACCTGTACTTGGCCAATAACAAGCT
  GACCTTGGCACCCCGCTTCCTGCCAAACGCCCTGATCAGTGTGGACTTTGCTGCCAACTATCTCACCAAG
  ATCTATGGGCTCACCTTTGGCCAGAAGCCAAACTTGAGGTCTGTGTACCTGCACAACAACAAGCTGGCAG
  ACGCCGGGCTGCCGGACAACATGTTCAACGGCTCCAGCAACGTCGAGGTCCTCATCCTGTCCAGCAACTT
  CCTGCGCCACGTGCCCAAGCACCTGCCGCCTGCCCTGTACAAGCTGCACCTCAAGAACAACAAGCTGGAG
  AAGATCCCCCCGGGGGCCTTCAGCGAGCTGAGCAGCCTGCGCGAGCTATACCTGCAGAACAACTACCTGA
  CTGACGAGGGCCTGGACAACGAGACCTTCTGGAAGCTCTCCAGCCTGGAGTACCTGGATCTGTCCAGCAA
  CAACCTGTCTCGGGTCCCAGCTGGGCTGCCGCGCAGCCTGGTGCTGCTGCACTTGGAGAAGAACGCCATC
  CGGAGCGTGGACGCGAATGTGCTGACCCCCATCCGCAGCCTGGAGTACCTGCTGCTGCACAGCAACCAGC
  TGCGGGAGCAGGGCATCCACCCACTGGCCTTCCAGGGCCTCAAGCGGTTGCACACGGTGCACCTGTACAA
  CAACGCGCTGGAGCGCGTGCCCAGTGGCCTGCCTCGCCGCGTGCGCACCCTCATGATCCTGCACAACCAG
  ATCACAGGCATTGGCCGCGAAGACTTTGCCACCACCTACTTCCTGGAGGAGCTCAACCTCAGCTACAACC
  GCATCACCAGCCCGCAGGTGCACCGCGACGCCTTCCGCAAGCTGCGCCTGCTGCGCTCGCTGGACCTGTC
  GGGCAACCGGCTGCACACGCTGCCACCTGGGCTGCCTCGAAATGTCCATGTGCTGAAGGTCAAGCGCAAT
  GAGCTGGCTGCCTTGGCACGAGGGGCGCTGGTGGGCATGGCTCAGCTGCGTGAGCTGTACCTCACCAGCA
  ACCGACTGCGCAGCCGAGCCCTGGGCCCCCGTGCCTGGGTGGACCTCGCCCATCTGCAGCTGCTGGACAT
  CGCCGGGAATCAGCTCACAGAGATCCCCGAGGGGCTCCCCGAGTCACTTGAGTACCTGTACCTGCAGAAC
  AACAAGATTAGTGCGGTGCCCGCCAATGCCTTCGACTCCACGCCCAACCTCAAGGGGATCTTTCTCAGGT
  TTAACAAGCTGGCTGTGGGCTCCGTGGTGGACAGTGCCTTCCGGAGGCTGAAGCACCTGCAGGTCTTGGA
  CATTGAAGGCAACTTAGAGTTTGGTGACATTTCCAAGGACCGTGGCCGCTTGGGGAAGGAAAAGGAGGAG
  GAGGAAGAGGAGGAGGAGGAGGAAGAGGAAACAAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC234637 representing NM_001199081
  Red=Cloning site Green=Tags(s)
  
  MEGEEAEQPAWFRQPWRPGASDSAPPAGTMAQSRVLLLLLLLPPQLHLGPVLAVRAPGFGRSGGHSLSPE
  ENEFAEEEPVLVLSPEEPGPGPAAVSCPRDCACSQEGVVDCGGIDLREFPGDLPEHTNHLSLQNNQLEKI
  YPEELSRLHRLETLNLQNNRLTSRGLPEKAFEHLTNLNYLYLANNKLTLAPRFLPNALISVDFAANYLTK
  IYGLTFGQKPNLRSVYLHNNKLADAGLPDNMFNGSSNVEVLILSSNFLRHVPKHLPPALYKLHLKNNKLE
  KIPPGAFSELSSLRELYLQNNYLTDEGLDNETFWKLSSLEYLDLSSNNLSRVPAGLPRSLVLLHLEKNAI
  RSVDANVLTPIRSLEYLLLHSNQLREQGIHPLAFQGLKRLHTVHLYNNALERVPSGLPRRVRTLMILHNQ
  ITGIGREDFATTYFLEELNLSYNRITSPQVHRDAFRKLRLLRSLDLSGNRLHTLPPGLPRNVHVLKVKRN
  ELAALARGALVGMAQLRELYLTSNRLRSRALGPRAWVDLAHLQLLDIAGNQLTEIPEGLPESLEYLYLQN
  NKISAVPANAFDSTPNLKGIFLRFNKLAVGSVVDSAFRRLKHLQVLDIEGNLEFGDISKDRGRLGKEKEE
  EEEEEEEEEETR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001199081
• ORF Size: 1926 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001199081.2
• RefSeq Size: 3075 bp
• RefSeq ORF: 1842 bp
• Locus ID: 127435
• UniProt ID: Q7Z5L7
• Cytogenetics: 1p32.3
• Protein Families: Secreted Protein
• MW: 72.1 kDa
• Gene Summary: The protein encoded by this gene is a member of the small leucine-rich repeat protein family and contains an amino terminal CX3CXCX7C cysteine-rich cluster followed by a leucine-rich repeat domain. Studies suggest that this protein could function to inhibit smooth muscle cell proliferation and migration following arterial injury. [provided by RefSeq, Jul 2016]