ABHD12B (NM_001206673) Human Tagged ORF Clone

CAT#: RC233878

ABHD12B (Myc-DDK tagged) - Homo sapiens abhydrolase domain containing 12B (ABHD12B), transcript variant 1

Product Images

Circular map for RC233878

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ABHD12B
• Synonyms: BEM46L3; C14orf29; c14_5314
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC233878 representing NM_001206673
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGCGCAGGACTGCCAGGCGGCCGCATCGCCCGAGCCGCCCGGGCCCCCAGCCCGTAGCTGCGTGG
  CCGCCTGGTGGGACATGGTCGACCGCAACCTGCGATATTTTCCACACTCCTGTTCAATGCTCGGAAGAAA
  AATTGCTGCTCTGTATGACAGCTTTACTAGTAAATCCTTAAAAGAACACGTTTTCCTTCCTCTGATAGAC
  ATGCTAATTTATTTTAATTTTTTCAAAGCCCCATTTCTTGTGGATTTAAAGAAACCAGAGTTAAAGATTC
  CTCACACAGTGAACTTCTACCTGAGAGTTGAACCTGGGGTGATGCTAGGGATCTGGCACACAGTCCCCAG
  CTGCCGGGGGGAAGATGCCAAGGGGAAGGACTGTTGCTGGTATGAAGCAGCCCTTCGTGATGGGAACCCA
  ATTATTGTTTATCTTCATGGCAGTGCAGAACACAGGGCAGCTTCGCACAGACTGAAGCTGGTAAAGGTGC
  TGAGTGATGGTGGCTTTCATGTCTTGTCTGTTGACTACAGAGGATTTGGGGACTCTACAGGTAAGCCCAC
  AGAGGAGGGACTGACTACGGATGCCATTTGTGTCTATGAGTGGACCAAGGCAAGAAGTGGCATCACTCCC
  GTGTGTCTCTGGGGCCACTCTCTGGGTACAGGAGTTGCAACAAATGCTGCAAAAGTGCTAGAAGAAAAAG
  GATGCCCAGTTGATGCTATTGTCTTGGAAGCTCCATTTACCAACATGTGGGTTGCAAGTATCAATTATCC
  CTTGTTAAAGATTTACCGGAACATTCCAGGATTTTTACGTACACTTATGGATGCCCTGAGAAAAGACAAA
  ATAATCTTTCCTAATGATGAAAATGTTAAATTCCTTTCTTCTCCTCTTCTCATCTTACATGGAGAGGATG
  ACAGGACAGTGCCTTTGGAGTATGGGAAAAAGCTCTATGAAATTGCACGCAATGCATACAGGAACAAAGA
  GAGGGTCAAGATGGTTATCTTTCCTCCTGGCTTCCAACACAACCTGCTTTGTAAAAGCCCCACACTGTTA
  ATAACCGTGAGAGATTTCCTGAGCAAGCAGTGGTCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC233878 representing NM_001206673
  Red=Cloning site Green=Tags(s)
  
  MDAQDCQAAASPEPPGPPARSCVAAWWDMVDRNLRYFPHSCSMLGRKIAALYDSFTSKSLKEHVFLPLID
  MLIYFNFFKAPFLVDLKKPELKIPHTVNFYLRVEPGVMLGIWHTVPSCRGEDAKGKDCCWYEAALRDGNP
  IIVYLHGSAEHRAASHRLKLVKVLSDGGFHVLSVDYRGFGDSTGKPTEEGLTTDAICVYEWTKARSGITP
  VCLWGHSLGTGVATNAAKVLEEKGCPVDAIVLEAPFTNMWVASINYPLLKIYRNIPGFLRTLMDALRKDK
  IIFPNDENVKFLSSPLLILHGEDDRTVPLEYGKKLYEIARNAYRNKERVKMVIFPPGFQHNLLCKSPTLL
  ITVRDFLSKQWS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001206673
• ORF Size: 1086 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001206673.2
• RefSeq Size: 1708 bp
• RefSeq ORF: 1089 bp
• Locus ID: 145447
• UniProt ID: Q7Z5M8
• Cytogenetics: 14q22.1
• MW: 41.2 kDa