CSNK1G3 (NM_001270574) Human Tagged ORF Clone

CAT#: RC233719

CSNK1G3 (Myc-DDK tagged) - Homo sapiens casein kinase 1, gamma 3 (CSNK1G3), transcript variant 7

Product Images

Circular map for RC233719

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CSNK1G3
• Synonyms: CKI-gamma 3; CSNK1G3L
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC233719 representing NM_001270574
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCTGGAACTGCTGGGACCTAGTTTGGAAGACTTGTTTGACTTGTGTGACAGAACATTTTCTCTTA
  AAACAGTTCTCATGATAGCTATACAACTGATTTCTCGCATGGAATATGTCCATTCAAAGAACTTGATATA
  CAGAGATGTAAAACCTGAGAACTTCTTAATAGGACGACCAGGAAACAAAACCCAGCAAGTTATTCACATT
  ATAGATTTTGGTTTGGCAAAGGAATATATTGATCCGGAGACAAAGAAACACATACCATACAGAGAACACA
  AGAGCCTTACAGGAACAGCTAGATATATGAGCATAAACACACATTTAGGAAAAGAACAAAGTAGAAGAGA
  CGATTTAGAAGCTTTAGGTCATATGTTCATGTATTTTCTGAGAGGCAGTCTTCCTTGGCAAGGCTTAAAG
  GCTGACACATTAAAGGAGAGGTATCAGAAAATTGGAGATACAAAACGGGCTACACCAATAGAAGTGTTAT
  GTGAAAATTTTCCAGAAGAAATGGCAACATATCTTCGTTATGTAAGAAGGCTAGATTTTTTTGAAAAACC
  AGACTATGACTACTTAAGAAAGCTTTTTACTGACTTGTTTGATCGAAAAGGATATATGTTTGATTATGAA
  TATGACTGGATTGGTAAACAGTTGCCTACTCCAGTGGGTGCAGTTCAGCAAGATCCTGCTCTGTCATCAA
  ACAGAGAAGCACATCAACACAGAGATAAGATGCAACAATCCAAAAACCAGGTTGTAAGTTCTACAAATGG
  AGAGTTAAACACAGATGACCCCACCGCAGGACGTTCAAATGCACCCATCACAGCCCCTACTGAAGTAGAA
  GTGATGGATGAAACCAACTGCCAGAAAGTGTTGAACATGTGGTGCTGCTGTTTTTTCAAACGAAGGAAAA
  GGAAAACCATACAGCGCCACAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC233719 representing NM_001270574
  Red=Cloning site Green=Tags(s)
  
  MVLELLGPSLEDLFDLCDRTFSLKTVLMIAIQLISRMEYVHSKNLIYRDVKPENFLIGRPGNKTQQVIHI
  IDFGLAKEYIDPETKKHIPYREHKSLTGTARYMSINTHLGKEQSRRDDLEALGHMFMYFLRGSLPWQGLK
  ADTLKERYQKIGDTKRATPIEVLCENFPEEMATYLRYVRRLDFFEKPDYDYLRKLFTDLFDRKGYMFDYE
  YDWIGKQLPTPVGAVQQDPALSSNREAHQHRDKMQQSKNQVVSSTNGELNTDDPTAGRSNAPITAPTEVE
  VMDETNCQKVLNMWCCCFFKRRKRKTIQRHK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001270574
• ORF Size: 933 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001270574.2
• RefSeq Size: 4118 bp
• RefSeq ORF: 936 bp
• Locus ID: 1456
• UniProt ID: Q9Y6M4
• Cytogenetics: 5q23.2
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Hedgehog signaling pathway
• MW: 36.9 kDa
• Gene Summary: This gene encodes a member of a family of serine/threonine protein kinases that phosphorylate caseins and other acidic proteins. A related protein in the African clawed frog participates in the transmission of Wnt/beta-catenin signaling. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]