PPP1R3B (NM_001201329) Human Tagged ORF Clone

CAT#: RC233646

PPP1R3B (Myc-DDK tagged) - Homo sapiens protein phosphatase 1, regulatory subunit 3B (PPP1R3B), transcript variant 1

Product Images

Circular map for RC233646

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PPP1R3B
• Synonyms: GL; PPP1R4; PTG
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC233646 representing NM_001201329
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGCTGTGGACATCGAGTACAGATACAACTGCATGGCTCCTTCCTTGCGCCAAGAGAGGTTTGCCT
  TTAAGATCTCACCAAAGCCCAGCAAACCACTGAGGCCTTGTATTCAGCTGAGCAGCAAGAATGAAGCCAG
  TGGAATGGTGGCCCCGGCTGTCCAGGAGAAGAAGGTGAAAAAGCGGGTGTCCTTCGCAGACAACCAGGGG
  CTGGCCCTGACAATGGTCAAAGTGTTCTCGGAATTCGATGACCCGCTAGATATGCCATTCAACATCACCG
  AGCTCCTAGACAACATTGTGAGCTTGACGACAGCAGAGAGCGAGAGCTTTGTTCTGGATTTTTCCCAGCC
  CTCTGCAGATTACTTAGACTTTAGAAATCGACTTCAGGCCGACCACGTCTGCCTTGAGAACTGTGTGCTC
  AAGGACAAGGCCATTGCAGGCACTGTGAAGGTTCAGAACCTCGCATTTGAGAAGACCGTGAAAATAAGGA
  TGACGTTCGACACCTGGAAGAGCTACACAGACTTTCCTTGTCAGTACGTGAAGGACACTTATGCCGGTTC
  AGACAGGGACACGTTCTCCTTCGACATCAGCTTGCCCGAGAAGATTCAGTCTTATGAAAGAATGGAGTTT
  GCTGTGTACTACGAGTGCAATGGACAGACGTACTGGGACAGCAACAGAGGCAAGAACTATAGGATCATCC
  GGGCTGAGTTAAAATCTACCCAGGGAATGACCAAGCCCCACAGTGGACCGGATTTGGGAATATCCTTTGA
  CCAGTTCGGAAGCCCTCGGTGTTCCTATGGTCTGTTTCCAGAGTGGCCAAGTTACTTAGGATATGAAAAG
  CTAGGGCCCTACTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC233646 representing NM_001201329
  Red=Cloning site Green=Tags(s)
  
  MMAVDIEYRYNCMAPSLRQERFAFKISPKPSKPLRPCIQLSSKNEASGMVAPAVQEKKVKKRVSFADNQG
  LALTMVKVFSEFDDPLDMPFNITELLDNIVSLTTAESESFVLDFSQPSADYLDFRNRLQADHVCLENCVL
  KDKAIAGTVKVQNLAFEKTVKIRMTFDTWKSYTDFPCQYVKDTYAGSDRDTFSFDISLPEKIQSYERMEF
  AVYYECNGQTYWDSNRGKNYRIIRAELKSTQGMTKPHSGPDLGISFDQFGSPRCSYGLFPEWPSYLGYEK
  LGPYY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001201329
• ORF Size: 855 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001201329.2
• RefSeq Size: 5722 bp
• RefSeq ORF: 858 bp
• Locus ID: 79660
• UniProt ID: Q86XI6
• Cytogenetics: 8p23.1
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Insulin signaling pathway
• MW: 33.1 kDa
• Gene Summary: This gene encodes the catalytic subunit of the serine/theonine phosphatase, protein phosphatase-1. The encoded protein is expressed in liver and skeletal muscle tissue and may be involved in regulating glycogen synthesis in these tissues. This gene may be a involved in type 2 diabetes and maturity-onset diabetes of the young. Alternate splicing results in multiple transcript variants that encode the same protein.[provided by RefSeq, Jan 2011]