NDUFS1 (NM_001199984) Human Tagged ORF Clone

CAT#: RC233112

NDUFS1 (Myc-DDK tagged) - Homo sapiens NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa (NADH-coenzyme Q reductase) (NDUFS1), transcript variant 5

Product Images

Circular map for RC233112

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NDUFS1
• Synonyms: CI-75k; CI-75Kd; MC1DN5; PRO1304
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC233112 representing NM_001199984
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGATCAGGGGGTCCTCGGGAACGCTGTCACGAATCAATATGTTAAGGATACCTGTAAGAAAGGCCT
  TAGTAGGCCTTTCTAAGTCTCCTAAAGGATGTGTTCGAACAACTGCCACAGCAGCAAGCAACTTGATTGA
  AGTATTTGTTGATGGTCAGTCTGTCATGGTGGAACCGGGAACGACCGTCCTCCAAGCTTGTGAGAAGGTT
  GGCATGCAGATCCCTCGATTCTGTTATCATGAAAGGTTGTCTGTTGCTGGAAACTGCAGGATGTGCCTTG
  TTGAAATTGAGAAAGCCCCTAAGGTTGTAGCTGCTTGTGCCATGCCAGTAATGAAGGGTTGGAATATCCT
  AACAAACTCAGAAAAATCCAAAAAAGCCAGGGAAGGTGTGATGGAGTTCTTATTAGCAAATCACCCATTG
  GACTGTCCTATTTGTGACCAGGGAGGTGAATGTGATCTGCAGGACCAGTCCATGATGTTTGGAAATGATA
  GGAGCCGATTTTTAGAGGGGAAGCGTGCTGTGGAAGACAAGAACATTGGGCCATTGGTAAAGACCATCAT
  GACAAGATGTATACAGTGTACTCGCTGCATCAGGTTTGCAAGTGAGATTGCAGGAGTAGATGATTTGGGA
  ACAACAGGCAGAGGAAATGATATGCAAGTTGGCACATACATTGAAAAGATGTTCATGTCTGAACTGTCTG
  GGAATATCATTGATATCTGCCCTGTAGGTGCCCTAACCTCTAAGCCCTATGCCTTTACTGCCCGGCCTTG
  GGAAACAAGAAAGACAGAATCCATTGATGTAATGGATGCGGTTGGAAGTAATATTGTGGTTAGCACAAGA
  ACTGGAGAAGTGATGAGGATTTTGCCACGTATGCATGAGGACATCAATGAAGAGTGGATCTCTGATAAAA
  CCAGATTTGCCTATGATGGGCTAAAACGTCAAAGACTTACCGAGCCAATGGTCAGAAATGAAAAAGGGCT
  TTTAACCTATACTTCTTGGGAGGATGCGCTCTCTCGCGTAGCTGGAATGTTGCAGAGTTTTCAAGGCAAA
  GATGTGGCAGCAATTGCAGGTGGCTTGGTGGATGCTGAAGCCCTGGTAGCTCTCAAAGATTTGCTTAATA
  GAGTGGACTCTGACACCTTATGCACTGAAGAGGTCTTCCCCACTGCAGGAGCTGGCACAGATTTGCGTTC
  CAATTATCTTCTTAATACTACAATTGCTGGTGTGGAAGAGGCAGATGTTGTTCTTCTGGTTGGTACAAAC
  CCACGTTTTGAGGCACCACTGTTTAATGCTAGAATTCGAAAGAGCTGGCTGCATAATGACTTAAAAGTGG
  CCCTTATAGGCAGTCCAGTGGACCTCACTTACACATATGACCACCTGGGAGACTCCCCCAAAATTCTTCA
  AGACATTGCTTCGGGAAGCCATCCATTTAGCCAGGTCCTAAAGGAAGCTAAAAAACCAATGGTGGTTTTA
  GGCAGTTCTGCACTCCAAAGAAATGATGGAGCAGCAATTCTTGCAGCTGTTTCTAGCATTGCACAAAAGA
  TTCGGATGACTAGTGGTGTTACTGGTGATTGGAAAGTTATGAATATCCTTCATAGGATTGCAAGTCAAGT
  AGCTGCTTTGGACCTTGGCTATAAGCCTGGGGTGGAAGCAATTCGGAAGAACCCTCCCAAGGTGCTGTTT
  CTCCTGGGAGCAGATGGAGGTTGTATCACACGACAGGATTTGCCAAAGGATTGTTTCATTATTTATCAAG
  GACATCATGGTGATGTTGGGGCTCCCATAGCTGATGTTATTCTCCCAGGAGCTGCTTACACAGAGAAGTC
  TGCTACATATGTCAACACTGAGGGTAGAGCTCAGCAGACTAAGGTAGCAGTGACACCTCCTGGCTTGGCA
  AGAGAAGACTGGAAAATTATAAGAGCACTCTCTGAGATTGCTGGAATGACTCTTCCATATGATACTCTGG
  ATCAAGTAAGGAACAGATTGGAAGAAGTCTCTCCTAATCTTGTTCGATATGATGATATTGAAGGGGCTAA
  TTACTTCCAGCAAGCAAATGAGCTCTCAAAGCTAGTGAACCAGCAGCTTCTTGCTGACCCACTTGTTCCA
  CCTCAGCTAACTATAAAAGACTTCTACATGACAGATTCAATTAGCAGAGCCTCACAGACAATGGCCAAAT
  GTGTCAAAGCTGTCACAGAGGGTGCCCAGGCAGTAGAGGAACCATCCATATGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC233112 representing NM_001199984
  Red=Cloning site Green=Tags(s)
  
  MRIRGSSGTLSRINMLRIPVRKALVGLSKSPKGCVRTTATAASNLIEVFVDGQSVMVEPGTTVLQACEKV
  GMQIPRFCYHERLSVAGNCRMCLVEIEKAPKVVAACAMPVMKGWNILTNSEKSKKAREGVMEFLLANHPL
  DCPICDQGGECDLQDQSMMFGNDRSRFLEGKRAVEDKNIGPLVKTIMTRCIQCTRCIRFASEIAGVDDLG
  TTGRGNDMQVGTYIEKMFMSELSGNIIDICPVGALTSKPYAFTARPWETRKTESIDVMDAVGSNIVVSTR
  TGEVMRILPRMHEDINEEWISDKTRFAYDGLKRQRLTEPMVRNEKGLLTYTSWEDALSRVAGMLQSFQGK
  DVAAIAGGLVDAEALVALKDLLNRVDSDTLCTEEVFPTAGAGTDLRSNYLLNTTIAGVEEADVVLLVGTN
  PRFEAPLFNARIRKSWLHNDLKVALIGSPVDLTYTYDHLGDSPKILQDIASGSHPFSQVLKEAKKPMVVL
  GSSALQRNDGAAILAAVSSIAQKIRMTSGVTGDWKVMNILHRIASQVAALDLGYKPGVEAIRKNPPKVLF
  LLGADGGCITRQDLPKDCFIIYQGHHGDVGAPIADVILPGAAYTEKSATYVNTEGRAQQTKVAVTPPGLA
  REDWKIIRALSEIAGMTLPYDTLDQVRNRLEEVSPNLVRYDDIEGANYFQQANELSKLVNQQLLADPLVP
  PQLTIKDFYMTDSISRASQTMAKCVKAVTEGAQAVEEPSIC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001199984
• ORF Size: 2223 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001199984.1, NP_001186913.1
• RefSeq Size: 3393 bp
• RefSeq ORF: 2226 bp
• Locus ID: 4719
• UniProt ID: P28331
• Cytogenetics: 2q33.3
• Protein Pathways: Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease
• MW: 81.4 kDa
• Gene Summary: The protein encoded by this gene belongs to the complex I 75 kDa subunit family. Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This protein is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized. Mutations in this gene are associated with complex I deficiency. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]