RUNX1T1 (NM_001198633) Human Tagged ORF Clone

CAT#: RC231324

RUNX1T1 (Myc-DDK-tagged)-Human runt-related transcription factor 1, translocated to, 1 (cyclin D-related) (RUNX1T1), transcript variant 13

Product Images

Circular map for RC231324

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RUNX1T1
• Synonyms: AML1-MTG8; AML1T1; CBFA2T1; CDR; ETO; MTG8; ZMYND2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC231324 representing NM_001198633
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGGACTCTCCGGCCCTGTGCAGTATCGTACTGAGAAGCACTCCACAATGCCAGACTCACCTGTGG
  ATGTGAAGACGCAATCTAGGCTGACTCCTCCAACAATGCCACCTCCCCCAACTACTCAAGGAGCTCCAAG
  AACCAGTTCATTTACACCGACAACGTTAACTAATGGCACGAGCCATTCTCCTACAGCCTTGAATGGCGCC
  CCCTCACCACCCAATGGCTTCAGCAATGGGCCTTCCTCTTCTTCCTCCTCCTCTCTGGCTAATCAACAGC
  TGCCCCCAGCCTGTGGTGCCAGGCAACTCAGCAAGCTGAAAAGGTTCCTTACTACCCTGCAGCAGTTTGG
  CAATGACATTTCACCCGAGATAGGAGAAAGAGTTCGCACCCTCGTTCTGGGACTAGTGAACTCCACTTTG
  ACAATTGAAGAATTTCATTCCAAACTGCAAGAAGCTACTAACTTCCCACTGAGACCTTTTGTCATCCCAT
  TTTTGAAGGCCAACTTGCCCCTGCTGCAGCGTGAGCTCCTCCACTGCGCAAGACTGGCCAAACAGAACCC
  TGCCCAGTACCTCGCCCAGCATGAACAGCTGCTTCTGGATGCCAGCACCACCTCACCTGTTGACTCCTCA
  GAGCTGCTTCTCGATGTGAACGAAAACGGGAAGAGGCGAACTCCAGACAGAACCAAAGAAAATGGCTTTG
  ACAGAGAGCCTTTGCACTCAGAACATCCAAGCAAGCGACCATGCACTATTAGCCCAGGCCAGCGGTACAG
  TCCAAATAACGGCTTATCCTACCAGCCCAATGGCCTGCCTCACCCTACCCCACCTCCACCTCAGCATTAC
  CGTTTGGATGATATGGCCATTGCCCACCACTACAGGGACTCCTATCGACACCCCAGCCACAGGGACCTCA
  GGGACAGAAACAGACCTATGGGGTTGCATGGCACACGTCAAGAAGAAATGATTGATCACAGACTAACAGA
  CAGAGAATGGGCAGAAGAGTGGAAACATCTTGACCATCTGTTAAACTGCATAATGGACATGGTAGAAAAA
  ACAAGGCGATCTCTCACCGTACTAAGGCGGTGTCAAGAAGCAGACCGGGAAGAATTGAATTACTGGATCC
  GGCGGTACAGTGACGCCGAGGACTTAAAAAAAGGTGGCGGCAGTAGCAGCAGCCACTCTAGGCAGCAGAG
  TCCCGTCAACCCAGACCCAGTTGCACTAGACGCGCATCGGGAATTCCTTCACAGGCCTGCGTCTGGATAC
  GTGCCAGAGGAGATCTGGAAGAAAGCTGAGGAGGCCGTCAATGAGGTGAAGCGCCAGGCGATGACGGAGC
  TGCAGAAGGCCGTGTCTGAGGCGGAGCGGAAAGCCCACGACATGATCACAACAGAGAGGGCCAAGATGGA
  GCGCACGGTCGCCGAGGCCAAACGGCAGGCGGCGGAGGACGCACTGGCAGTTATCAATCAGCAGGAGGAT
  TCAAGCGAGAGTTGCTGGAATTGTGGCCGTAAAGCGAGTGAAACCTGCAGTGGCTGTAACACAGCCCGAT
  ACTGTGGCTCATTTTGCCAGCACAAAGACTGGGAGAAGCACCATCACATCTGTGGACAGACCCTGCAGGC
  CCAGCAGCAGGGAGACACACCTGCAGTCAGCTCCTCTGTCACGCCCAACAGCGGGGCTGGGAGCCCGATG
  GACACACCACCAGCAGCCACTCCGAGGTCAACCACCCCGGGAACCCCTTCCACCATAGAGACAACCCCTC
  GC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC231324 representing NM_001198633
  Red=Cloning site Green=Tags(s)
  
  MVGLSGPVQYRTEKHSTMPDSPVDVKTQSRLTPPTMPPPPTTQGAPRTSSFTPTTLTNGTSHSPTALNGA
  PSPPNGFSNGPSSSSSSSLANQQLPPACGARQLSKLKRFLTTLQQFGNDISPEIGERVRTLVLGLVNSTL
  TIEEFHSKLQEATNFPLRPFVIPFLKANLPLLQRELLHCARLAKQNPAQYLAQHEQLLLDASTTSPVDSS
  ELLLDVNENGKRRTPDRTKENGFDREPLHSEHPSKRPCTISPGQRYSPNNGLSYQPNGLPHPTPPPPQHY
  RLDDMAIAHHYRDSYRHPSHRDLRDRNRPMGLHGTRQEEMIDHRLTDREWAEEWKHLDHLLNCIMDMVEK
  TRRSLTVLRRCQEADREELNYWIRRYSDAEDLKKGGGSSSSHSRQQSPVNPDPVALDAHREFLHRPASGY
  VPEEIWKKAEEAVNEVKRQAMTELQKAVSEAERKAHDMITTERAKMERTVAEAKRQAAEDALAVINQQED
  SSESCWNCGRKASETCSGCNTARYCGSFCQHKDWEKHHHICGQTLQAQQQGDTPAVSSSVTPNSGAGSPM
  DTPPAATPRSTTPGTPSTIETTPR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001198633
• ORF Size: 1752 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001198633.1, NP_001185562.1
• RefSeq ORF: 1755 bp
• Locus ID: 862
• UniProt ID: Q06455
• Cytogenetics: 8q21.3
• Protein Families: Transcription Factors
• Protein Pathways: Acute myeloid leukemia, Pathways in cancer
• MW: 65.5 kDa
• Gene Summary: This gene encodes a member of the myeloid translocation gene family which interact with DNA-bound transcription factors and recruit a range of corepressors to facilitate transcriptional repression. The t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities in acute myeloid leukemia. The translocation produces a chimeric gene made up of the 5'-region of the runt-related transcription factor 1 gene fused to the 3'-region of this gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2010]