GTPBP3 (NM_001195422) Human Tagged ORF Clone

CAT#: RC231285

GTPBP3 (Myc-DDK-tagged)-Human GTP binding protein 3 (mitochondrial) (GTPBP3), transcript variant VII

Product Images

Circular map for RC231285

• 

  Western blot validation of overexpression lysate (Cat# LY434284) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC231285 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GTPBP3
• Synonyms: COXPD23; GTPBG3; MSS1; MTGP1; THDF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC231285 representing NM_001195422
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCATTCTCCAACTTGTCCCCACCCATGCTTTCTTCTGGTCCCCGCCTCGGAGCCTCAGTTTCCCC
  ATCTGCAAACTCCGGACCCTGGAGACGCTGTTTGGAATGTGAGATGGGCATTGTGCACGCGCCGGAGCAG
  CGGCGCACCAGCCCCCGGCTCCGGCGCCACCATCTTCGCGCTAAGCTCTGGCCAAGGCCGCTGCGGCATC
  GCAGTGATCCGGACCAGCGGCCCCGCCAGCGGCCACGCCCTCCGAATTCTCACAGCACCCCGAGACCTGC
  CCCTTGCTCGCCACGCCAGCCTGCGCCTGCTCAGCGATCCCCGCTCCGGGGAGCCTCTGGACCGCGCACT
  GGTGCTCTGGTTCCCAGGTCCCCAGAGTTTCACCGGTGAGGACTGCGTGGAGTTCCACGTGCATGGAGGC
  CCGGCAGTGGTGAGCGGCGTCCTGCAGGCCTTGGGCAGCGTGCCAGGGCTTCGACCGGCGGAGGCAGGCG
  AGTTCACCAGACGGGCGTTCGCCAATGGGAAGCTGAACCTGACCGAAGTGGAGGGGCTGGCGGACCTTAT
  CCACGCGGAAACAGAGGCGCAGCGGCGGCAGGCCCTCAGGCAGCTGGACGGAGAGCTGGGCCACCTCTGC
  CGTGGCTGGGCCGAGACCCTCACCAAAGCTCTGGCCCACGTGGAGGCCTATATCGATTTCGGCGAGGATG
  ACAACCTGGAGGAGGGGGTCCTGGAGCAAGCCGACATCGAAGTACGGGCACTGCAGGTGGCCCTGGGTGC
  ACATCTACGAGATGCCAGGCGCGGGCAGAGGCTCCGCTCAGGGGTGCACGTAGTGGTCACTGGACCCCCC
  AATGCGGGCAAGAGCAGCCTAGTGAACCTGCTCAGTCGGAAGCCTGTGTCCATCGTGTCCCCGGAGCCAG
  GGACCACCCGTGACGTGCTGGAGACCCCAGTCGACCTGGCCGGATTTCCTGTGCTGCTGAGCGACACGGC
  TGGGTTGCGGGAGGGCGTGGGGCCCGTGGAGCAGGAGGGCGTGCGGCGCGCCCGGGAGAGGCTAGAGCAG
  GCTGACCTCATTCTGGCCATGCTGGATGCTTCTGACCTGGCCTCTCCCTCCAGTTGCAACTTCCTGGCCA
  CCGTCGTAGCCTCTGTGGGAGCCCAGAGCCCCAGTGACAGCAGCCAGCGCCTCCTCCTGGTGCTGAACAA
  GTCGGACCTGCTGTCCCCGGAGGGCCCAGGTCCCGGTCCTGACCTGCCCCCGCACCTGCTGCTGTCCTGT
  CTGACGGGAGAGGGGCTGGACGGCCTCCTGGAGGCGCTGAGGAAGGAGCTAGCTGCAGTGTGTGGGGACC
  CGTCCACAGATCCCCCGCTGCTGACCCGAGCAAGGCACCAGCACCACCTCCAGGGTTGCCTGGATGCCCT
  CGGCCACTACAAGCAGTCAAAAGACCTGGCCCTGGCGGCAGAGGCGCTGCGGGTGGCCCGGGGTCACCTG
  ACCCGGCTCACAGGTGGAGGGGGTACCGAGGAGATCCTGGACATCATCTTCCAGGACTTCTGTGTGGGCA
  AG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC231285 representing NM_001195422
  Red=Cloning site Green=Tags(s)
  
  MVHSPTCPHPCFLLVPASEPQFPHLQTPDPGDAVWNVRWALCTRRSSGAPAPGSGATIFALSSGQGRCGI
  AVIRTSGPASGHALRILTAPRDLPLARHASLRLLSDPRSGEPLDRALVLWFPGPQSFTGEDCVEFHVHGG
  PAVVSGVLQALGSVPGLRPAEAGEFTRRAFANGKLNLTEVEGLADLIHAETEAQRRQALRQLDGELGHLC
  RGWAETLTKALAHVEAYIDFGEDDNLEEGVLEQADIEVRALQVALGAHLRDARRGQRLRSGVHVVVTGPP
  NAGKSSLVNLLSRKPVSIVSPEPGTTRDVLETPVDLAGFPVLLSDTAGLREGVGPVEQEGVRRARERLEQ
  ADLILAMLDASDLASPSSCNFLATVVASVGAQSPSDSSQRLLLVLNKSDLLSPEGPGPGPDLPPHLLLSC
  LTGEGLDGLLEALRKELAAVCGDPSTDPPLLTRARHQHHLQGCLDALGHYKQSKDLALAAEALRVARGHL
  TRLTGGGGTEEILDIIFQDFCVGK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001195422
• ORF Size: 1542 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001195422.1, NP_001182351.1
• RefSeq ORF: 1545 bp
• Locus ID: 84705
• UniProt ID: Q969Y2
• Cytogenetics: 19p13.11
• MW: 54.9 kDa
• Gene Summary: This locus encodes a GTP-binding protein. The encoded protein is localized to the mitochondria and may play a role in mitochondrial tRNA modification. Polymorphisms at this locus may be associated with severity of aminoglycoside-induced deafness, a disease associated with a mutation in the 12S rRNA. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Sep 2010]