CHPF (NM_001195731) Human Tagged ORF Clone

CAT#: RC230858

CHPF (Myc-DDK-tagged)-Human chondroitin polymerizing factor (CHPF), transcript variant 2

Product Images

Circular map for RC230858

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CHPF
• Synonyms: CHSY2; CSS2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230858 representing NM_001195731
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGTGGTGACGCTAGGCGAGGAGCGACCCATTGGACACCTGCACCTGGCGCTGCGCCACCTGCTGG
  AGCAGCACGGCGACGACTTTGACTGGTTCTTCCTGGTGCCTGACACCACCTACACCGAGGCGCACGGCCT
  GGCACGCCTAACTGGCCACCTCAGCCTGGCCTCCGCCGCCCACCTGTACCTGGGCCGGCCCCAGGACTTC
  ATCGGCGGAGAGCCCACCCCCGGCCGCTACTGCCACGGAGGCTTTGGGGTGCTGCTGTCGCGCATGCTGC
  TGCAACAACTGCGCCCCCACCTGGAAGGCTGCCGCAACGACATCGTCAGTGCGCGCCCTGACGAGTGGCT
  GGGTCGCTGCATTCTCGATGCCACCGGGGTGGGCTGCACTGGTGACCACGAGGGGGTGCACTATAGCCAT
  CTGGAGCTGAGCCCTGGGGAGCCAGTGCAGGAGGGGGACCCTCATTTCCGAAGTGCCCTGACAGCCCACC
  CTGTGCGTGACCCTGTGCACATGTACCAGCTGCACAAAGCTTTCGCCCGAGCTGAACTGGAACGCACGTA
  CCAGGAGATCCAGGAGTTACAGTGGGAGATCCAGAATACCAGCCATCTGGCCGTTGATGGGGACCGGGCA
  GCTGCTTGGCCCGTGGGTATTCCAGCACCATCCCGCCCGGCCTCCCGCTTTGAGGTGCTGCGCTGGGACT
  ACTTCACGGAGCAGCACGCTTTCTCCTGCGCCGATGGCTCACCCCGCTGCCCACTGCGTGGGGCTGACCG
  GGCTGATGTGGCCGATGTTCTGGGGACAGCTCTAGAGGAGCTGAACCGCCGCTACCACCCGGCCTTGCGG
  CTCCAGAAGCAGCAGCTGGTGAATGGCTACCGACGCTTTGATCCGGCCCGGGGTATGGAATACACGCTGG
  ACTTGCAGCTGGAGGCACTGACCCCCCAGGGAGGCCGCCGGCCCCTCACTCGCCGAGTGCAGCTGCTCCG
  GCCGCTGAGCCGCGTGGAGATCTTGCCTGTGCCCTATGTCACTGAGGCCTCACGTCTCACTGTGCTGCTG
  CCTCTAGCTGCGGCTGAGCGTGACCTGGCCCCTGGCTTCTTGGAGGCCTTTGCCACTGCAGCACTGGAGC
  CTGGTGATGCTGCGGCAGCCCTGACCCTGCTGCTACTGTATGAGCCGCGCCAGGCCCAGCGCGTGGCCCA
  TGCAGATGTCTTCGCACCTGTCAAGGCCCACGTGGCAGAGCTGGAGCGGCGTTTCCCCGGTGCCCGGGTG
  CCATGGCTCAGTGTGCAGACAGCCGCACCCTCACCACTGCGCCTCATGGATCTACTCTCCAAGAAGCACC
  CGCTGGACACACTGTTCCTGCTGGCCGGGCCAGACACGGTGCTCACGCCTGACTTCCTGAACCGCTGCCG
  CATGCATGCCATCTCCGGCTGGCAGGCCTTCTTTCCCATGCATTTCCAAGCCTTCCACCCAGCTGTGGCC
  CCACCACAAGGGCCTGGGCCCCCAGAGCTGGGCCGTGACACTGGCCGCTTTGATCGCCAGGCAGCCAGCG
  AGGCCTGCTTCTACAACTCCGACTACGTGGCAGCCCGTGGGCGCCTGGCGGCAGCCTCAGAACAAGAAGA
  GGAGCTGCTGGAGAGCCTGGATGTGTACGAGCTGTTCCTCCACTTCTCCAGTCTGCATGTGCTGCGGGCG
  GTGGAGCCGGCGCTGCTGCAGCGCTACCGGGCCCAGACGTGCAGCGCGAGGCTCAGTGAGGACCTGTACC
  ACCGCTGCCTCCAGAGCGTGCTTGAGGGCCTCGGCTCCCGAACCCAGCTGGCCATGCTACTCTTTGAACA
  GGAGCAGGGCAACAGCACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230858 representing NM_001195731
  Red=Cloning site Green=Tags(s)
  
  MAVVTLGEERPIGHLHLALRHLLEQHGDDFDWFFLVPDTTYTEAHGLARLTGHLSLASAAHLYLGRPQDF
  IGGEPTPGRYCHGGFGVLLSRMLLQQLRPHLEGCRNDIVSARPDEWLGRCILDATGVGCTGDHEGVHYSH
  LELSPGEPVQEGDPHFRSALTAHPVRDPVHMYQLHKAFARAELERTYQEIQELQWEIQNTSHLAVDGDRA
  AAWPVGIPAPSRPASRFEVLRWDYFTEQHAFSCADGSPRCPLRGADRADVADVLGTALEELNRRYHPALR
  LQKQQLVNGYRRFDPARGMEYTLDLQLEALTPQGGRRPLTRRVQLLRPLSRVEILPVPYVTEASRLTVLL
  PLAAAERDLAPGFLEAFATAALEPGDAAAALTLLLLYEPRQAQRVAHADVFAPVKAHVAELERRFPGARV
  PWLSVQTAAPSPLRLMDLLSKKHPLDTLFLLAGPDTVLTPDFLNRCRMHAISGWQAFFPMHFQAFHPAVA
  PPQGPGPPELGRDTGRFDRQAASEACFYNSDYVAARGRLAAASEQEEELLESLDVYELFLHFSSLHVLRA
  VEPALLQRYRAQTCSARLSEDLYHRCLQSVLEGLGSRTQLAMLLFEQEQGNST
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001195731
• ORF Size: 1839 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001195731.1, NP_001182660.1
• RefSeq Size: 2657 bp
• RefSeq ORF: 1842 bp
• Locus ID: 79586
• UniProt ID: Q8IZ52
• Cytogenetics: 2q35
• Protein Families: Druggable Genome
• Protein Pathways: Chondroitin sulfate biosynthesis, Metabolic pathways
• MW: 68.4 kDa
• Gene Summary: Has both beta-1,3-glucuronic acid and beta-1,4-N-acetylgalactosamine transferase activity. Transfers glucuronic acid (GlcUA) from UDP-GlcUA and N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of the elongating chondroitin polymer. Isoform 2 may facilitate PRKN transport into the mitochondria. In collaboration with PRKN, isoform 2 may enhance cell viability and protect cells from oxidative stress.[UniProtKB/Swiss-Prot Function]