GPR64 (ADGRG2) (NM_001184835) Human Tagged ORF Clone

CAT#: RC230599

GPR64 (Myc-DDK-tagged)-Human G protein-coupled receptor 64 (GPR64), transcript variant 7

Product Images

Circular map for RC230599

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GPR64
• Synonyms: CBAVDX; EDDM6; GPR64; HE6; TM7LN2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230599 representing NM_001184835
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTTTCTCTGTCAGGCAGTGTGGCCATGTTGGCAGAACTGAAGAAGTTTTACTGACGTTCAAGATAT
  TCCTTGTCATCATTTGTCTTCATGTCGTTCTGGTAACATCCCTGGAAGAAGATACTGATAATTCCAGTTT
  GTCACCACCACCTGATGTTACTTTAAGCTTACTCCCTTCAAACGAAACAGGCGTCAAACCCCAGAGAAAT
  ATCTGCAATTTGTCATCTATTTGCAATGACTCAGCATTTTTTAGAGGTGAGATCATGTTTCAATATGATA
  AAGAAAGCACTGTTCCCCAGAATCAACATATAACGAATGGCACCTTAACTGGAGTCCTGTCTCTAAGTGA
  ATTAAAACGCTCAGAGCTCAACAAAACCCTGCAAACCCTAAGTGAGACTTACTTTATAATGTGTGCTACA
  GCAGAGGCCCAAAGCACATTAAATTGTACATTCACAATAAAACTGAATAATACAATGAATGCATGTGCTG
  TAATAGCTGCTTTGGAAAGAGTAAAGATTCGACCAATGGAACACTGCTGCTGTTCTGTCAGGATACCCTG
  CCCTTCCTCCCCAGAAGAGTTGGAAAAGCTTCAGTGTGACCTGCAGGATCCCATTGTCTGTCTTGCTGAC
  CATCCACGTGGCCCACCATTTTCTTCCAGCCAATCCATCCCAGTGGTGCCTCGGGCCACTGTGCTTTCCC
  AGGTCCCCAAAGCTACCTCTTTTGCTGAGCCTCCAGATTATTCACCTGTGACCCACAATGTTCCCTCTCC
  AATAGGGGAGATTCAACCCCTTTCACCCCAGCCTTCAGCTCCCATAGCTTCCAGCCCTGCCATTGACATG
  CCCCCACAGTCTGAAACGATCTCTTCCCCTATGCCCCAAACCCATGTCTCCGGCACCCCACCTCCTGTGA
  AAGCCTCATTTTCCTCTCCCACCGTGTCTGCCCCTGCGAATGTCAACACTACCAGCGCACCTCCTGTCCA
  GACAGACATCGTCAACACCAGCAGTATTTCTGATCTTGAGAACCAAGTGTTGCAGATGGAGAAGGCTCTG
  TCCTTGGGCAGCCTGGAGCCTAACCTCGCAGGAGAAATGATCAACCAAGTCAGCAGACTCCTTCATTCCC
  CGCCTGACATGCTGGCCCCTCTGGCTCAAAGATTGCTGAAAGTAGTGGATGACATTGGCCTACAGCTGAA
  CTTTTCAAACACGACTATAAGTCTAACCTCCCCTTCTTTGGCTCTGGCTGTGATCAGAGTGAATGCCAGT
  AGTTTCAACACAACTACCTTTGTGGCCCAAGACCCTGCAAATCTTCAGGTTTCTCTGGAAACCCAAGCTC
  CTGAGAACAGTATTGGCACAATTACTCTTCCTTCATCGCTGATGAATAATTTACCAGCTCATGACATGGA
  GCTAGCTTCCAGGGTTCAGTTCAATTTTTTTGAAACACCTGCTTTGTTTCAGGATCCTTCCCTGGAGAAC
  CTCTCTCTGATCAGCTACGTCATATCATCGAGTGTTGCAAACCTGACCGTCAGGAACTTGACAAGAAACG
  TGACAGTCACATTAAAGCACATCAACCCGAGCCAGGATGAGTTAACAGTGAGATGTGTATTTTGGGACTT
  GGGCAGAAATGGTGGCAGAGGAGGCTGGTCAGACAATGGCTGCTCTGTCAAAGACAGGAGATTGAATGAA
  ACCATCTGTACCTGTAGCCATCTAACAAGCTTCGGCGTTCTGCTGGACCTATCTAGGACATCTGTGCTGC
  CTGCTCAAATGATGGCTCTGACGTTCATTACATATATTGGTTGTGGGCTTTCATCAATTTTTCTGTCAGT
  GACTCTTGTAACCTACATAGCTTTTGAAAAGATCCGGAGGGATTACCCTTCCAAAATCCTCATCCAGCTG
  TGTGCTGCTCTGCTTCTGCTGAACCTGGTCTTCCTCCTGGACTCGTGGATTGCTCTGTATAAGATGCAAG
  GCCTCTGCATCTCAGTGGCTGTATTTCTTCATTATTTTCTCTTGGTCTCATTCACATGGATGGGCCTAGA
  AGCATTCCATATGTACCTGGCCCTTGTCAAAGTATTTAATACTTACATCCGAAAATACATCCTTAAATTC
  TGCATTGTCGGTTGGGGGGTACCAGCTGTGGTTGTGACCATCATCCTGACTATATCCCCAGATAACTATG
  GGCTTGGATCCTATGGGAAATTCCCCAATGGTTCACCGGATGACTTCTGCTGGATCAACAACAATGCAGT
  ATTCTACATTACGGTGGTGGGATATTTCTGTGTGATATTTTTGCTGAACGTCAGCATGTTCATTGTGGTC
  CTGGTTCAGCTCTGTCGAATTAAAAAGAAGAAGCAACTGGGAGCCCAGCGAAAAACCAGTATTCAAGACC
  TCAGGAGTATCGCTGGCCTTACATTTTTACTGGGAATAACTTGGGGCTTTGCCTTCTTTGCCTGGGGACC
  AGTTAACGTGACCTTCATGTATCTGTTTGCCATCTTTAATACCTTACAAGGATTTTTCATATTCATCTTT
  TACTGTGTGGCCAAAGAAAATGTCAGGAAGCAATGGAGGCGGTATCTTTGTTGTGGAAAGTTACGGCTGG
  CTGAAAATTCTGACTGGAGTAAAACTGCTACTAATGGTTTAAAGAAGCAGACTGTAAACCAAGGAGTGTC
  CAGCTCTTCAAATTCCTTACAGTCAAGCAGTAACTCCACTAACTCCACCACACTGCTAGTGAATAATGAT
  TGCTCAGTACACGCAAGCGGGAATGGAAATGCTTCTACAGAGAGGAATGGGGTCTCTTTTAGTGTTCAGA
  ATGGAGATGTGTGCCTTCACGATTTCACTGGAAAACAGCACATGTTTAACGAGAAGGAAGATTCCTGCAA
  TGGGAAAGGCCGTATGGCTCTCAGAAGGACTTCAAAGCGGGGAAGCTTACACTTTATTGAGCAAATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230599 representing NM_001184835
  Red=Cloning site Green=Tags(s)
  
  MVFSVRQCGHVGRTEEVLLTFKIFLVIICLHVVLVTSLEEDTDNSSLSPPPDVTLSLLPSNETGVKPQRN
  ICNLSSICNDSAFFRGEIMFQYDKESTVPQNQHITNGTLTGVLSLSELKRSELNKTLQTLSETYFIMCAT
  AEAQSTLNCTFTIKLNNTMNACAVIAALERVKIRPMEHCCCSVRIPCPSSPEELEKLQCDLQDPIVCLAD
  HPRGPPFSSSQSIPVVPRATVLSQVPKATSFAEPPDYSPVTHNVPSPIGEIQPLSPQPSAPIASSPAIDM
  PPQSETISSPMPQTHVSGTPPPVKASFSSPTVSAPANVNTTSAPPVQTDIVNTSSISDLENQVLQMEKAL
  SLGSLEPNLAGEMINQVSRLLHSPPDMLAPLAQRLLKVVDDIGLQLNFSNTTISLTSPSLALAVIRVNAS
  SFNTTTFVAQDPANLQVSLETQAPENSIGTITLPSSLMNNLPAHDMELASRVQFNFFETPALFQDPSLEN
  LSLISYVISSSVANLTVRNLTRNVTVTLKHINPSQDELTVRCVFWDLGRNGGRGGWSDNGCSVKDRRLNE
  TICTCSHLTSFGVLLDLSRTSVLPAQMMALTFITYIGCGLSSIFLSVTLVTYIAFEKIRRDYPSKILIQL
  CAALLLLNLVFLLDSWIALYKMQGLCISVAVFLHYFLLVSFTWMGLEAFHMYLALVKVFNTYIRKYILKF
  CIVGWGVPAVVVTIILTISPDNYGLGSYGKFPNGSPDDFCWINNNAVFYITVVGYFCVIFLLNVSMFIVV
  LVQLCRIKKKKQLGAQRKTSIQDLRSIAGLTFLLGITWGFAFFAWGPVNVTFMYLFAIFNTLQGFFIFIF
  YCVAKENVRKQWRRYLCCGKLRLAENSDWSKTATNGLKKQTVNQGVSSSSNSLQSSSNSTNSTTLLVNND
  CSVHASGNGNASTERNGVSFSVQNGDVCLHDFTGKQHMFNEKEDSCNGKGRMALRRTSKRGSLHFIEQM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001184835
• ORF Size: 2937 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001184835.2
• RefSeq ORF: 2940 bp
• Locus ID: 10149
• UniProt ID: Q8IZP9
• Cytogenetics: Xp22.13
• Protein Families: Druggable Genome, GPCR, Transmembrane
• MW: 108.1 kDa
• Gene Summary: This gene encodes a member of the G protein-coupled receptor family described as an epididymis-specific transmembrane protein. The encoded protein may be proteolytically processed as it contains a motif shown to be a protein scission motif in some members of this family (PMID: 11973329). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]