BCAR1 (NM_001170714) Human Tagged ORF Clone

CAT#: RC230582

BCAR1 (Myc-DDK-tagged)-Human breast cancer anti-estrogen resistance 1 (BCAR1), transcript variant 1

Product Images

Circular map for RC230582

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BCAR1
• Synonyms: CAS; CAS1; CASS1; CRKAS; P130Cas
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230582 representing NM_001170714
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTGCCAAGCCCTTCCTCTCTTCTGTTCTGCTCTCCTGGAAAGTTCTGGACTTCTCTGGCCCAGGGC
  CTCAGGGGACTGGCCAGCCCTGCTCCTGTGGGCACTGGGCAGAGGGACAAGGCGGACCACCTGAGCCTGC
  TGGAGGGCCGAACGTGCTGGCCAAAGCGCTCTATGACAATGTGGCCGAGTCCCCGGATGAGCTCTCCTTC
  CGCAAGGGTGACATCATGACGGTGCTGGAGCAGGACACGCAGGGCCTGGACGGCTGGTGGCTCTGCTCGC
  TGCATGGGCGCCAGGGCATCGTGCCTGGGAACCGCCTCAAGATCTTGGTGGGCATGTATGATAAGAAGCC
  AGCAGGGCCTGGCCCCGGCCCTCCCGCCACCCCGGCCCAGCCTCAGCCTGGCCTCCATGCCCCAGCGCCT
  CCGGCCTCCCAGTACACGCCCATGCTCCCCAACACCTACCAGCCCCAGCCAGACAGCGTCTACCTGGTGC
  CCACTCCCAGCAAGGCTCAGCAAGGCCTCTACCAAGTCCCGGGTCCCAGCCCTCAGTTCCAGTCTCCCCC
  AGCCAAGCAGACATCCACCTTCTCGAAGCAGACACCCCATCACCCGTTTCCCAGCCCGGCCACAGACCTG
  TACCAGGTGCCCCCAGGGCCTGGAGGCCCTGCCCAGGATATTTACCAGGTGCCACCTTCTGCCGGGATGG
  GGCATGACATCTACCAGGTCCCCCCGTCCATGGACACACGCAGCTGGGAGGGCACGAAGCCCCCGGCAAA
  GGTGGTGGTGCCCACCCGCGTGGGGCAGGGCTATGTATACGAGGCCGCCCAGCCGGAGCAGGACGAGTAC
  GACATCCCGCGACACCTGCTGGCCCCGGGGCCACAGGACATCTATGATGTGCCCCCGGTTCGGGGGCTGC
  TTCCCAGCCAGTATGGCCAGGAGGTGTATGACACACCCCCCATGGCTGTCAAGGGTCCCAATGGCCGAGA
  CCCGTTGCTGGAGGTGTATGACGTGCCCCCCAGTGTGGAGAAGGGCCTGCCACCGTCCAACCACCACGCA
  GTCTACGACGTTCCTCCATCGGTGAGCAAGGATGTGCCCGATGGCCCACTGCTGCGTGAGGAGACCTACG
  ATGTGCCCCCCGCCTTCGCCAAGGCCAAGCCCTTTGACCCGGCCCGCACCCCACTGGTACTGGCTGCGCC
  CCCTCCAGACTCCCCGCCGGCCGAGGACGTGTATGACGTGCCGCCCCCGGCTCCTGACCTCTACGACGTG
  CCCCCTGGCTTGCGGCGGCCTGGCCCGGGCACCCTGTACGATGTGCCCCGTGAACGGGTGCTTCCTCCTG
  AGGTGGCTGATGGTGGCGTGGTCGACAGTGGTGTGTATGCGGTGCCTCCCCCAGCTGAACGTGAAGCCCC
  GGCAGAGGGCAAGCGCCTGTCGGCCTCCAGCACCGGCAGCACACGCAGCAGCCAGTCTGCGTCCTCCTTG
  GAGGTGGCAGGGCCGGGCCGGGAACCCCTGGAGCTGGAAGTTGCTGTGGAGGCCCTGGCACGGCTGCAGC
  AGGGTGTGAGCGCCACCGTTGCCCACCTTCTGGACCTGGCAGGCAGCGCCGGTGCGACTGGGAGCTGGCG
  TAGCCCCTCTGAGCCACAGGAGCCGCTGGTGCAGGACCTGCAGGCTGCTGTGGCCGCTGTCCAGAGTGCC
  GTCCACGAGCTGTTGGAGTTTGCCCGCAGCGCGGTGGGCAATGCTGCCCACACATCTGACCGTGCCCTGC
  ATGCCAAGCTTAGCCGGCAGCTGCAGAAGATGGAGGACGTGCACCAGACGCTGGTGGCACATGGTCAGGC
  CCTCGACGCTGGCCGGGGAGGCTCTGGAGCCACCCTTGAGGACCTGGACCGGCTGGTGGCCTGCTCGCGG
  GCTGTGCCCGAGGACGCCAAGCAGCTGGCCTCCTTCCTGCACGGCAATGCCTCACTGCTCTTCAGACGGA
  CCAAGGCCACTGCCCCGGGGCCTGAGGGGGGTGGCACCCTGCACCCCAACCCCACTGACAAGACCAGCAG
  CATCCAGTCACGACCCCTGCCCTCACCCCCTAAGTTCACCTCCCAGGACTCGCCAGATGGGCAGTACGAG
  AACAGCGAGGGGGGCTGGATGGAGGACTATGACTACGTCCACCTACAGGGGAAGGAGGAGTTTGAGAAGA
  CCCAGAAGGAGCTGCTGGAAAAGGGCAGCATCACGCGGCAGGGCAAGAGCCAGCTGGAGTTGCAGCAGCT
  GAAGCAGTTTGAACGACTGGAACAGGAGGTGTCACGGCCCATAGACCACGACCTGGCCAACTGGACGCCA
  GCCCAACCCCTGGCCCCGGGGCGAACAGGCGGCCTGGGGCCCTCGGACCGGCAGCTGCTGCTCTTCTACC
  TGGAGCAGTGTGAGGCCAACCTGACCACACTGACCAACGCCGTGGACGCCTTCTTTACCGCCGTGGCCAC
  CAACCAGCCGCCCAAGATCTTTGTGGCGCACAGCAAGTTCGTCATCCTCAGCGCCCACAAGCTGGTGTTC
  ATCGGGGACACACTGTCACGGCAGGCCAAGGCTGCTGACGTGCGCAGCCAGGTGACCCACTACAGCAACC
  TGCTGTGCGACCTCCTGCGCGGCATCGTGGCCACCACCAAGGCCGCTGCCTTGCAGTACCCATCGCCTTC
  CGCGGCCCAGGACATGGTGGAGAGGGTCAAGGAGCTGGGCCACAGCACCCAGCAGTTCCGCCGCGTCCTA
  GGCCAGCTGGCAGCCGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230582 representing NM_001170714
  Red=Cloning site Green=Tags(s)
  
  MPAKPFLSSVLLSWKVLDFSGPGPQGTGQPCSCGHWAEGQGGPPEPAGGPNVLAKALYDNVAESPDELSF
  RKGDIMTVLEQDTQGLDGWWLCSLHGRQGIVPGNRLKILVGMYDKKPAGPGPGPPATPAQPQPGLHAPAP
  PASQYTPMLPNTYQPQPDSVYLVPTPSKAQQGLYQVPGPSPQFQSPPAKQTSTFSKQTPHHPFPSPATDL
  YQVPPGPGGPAQDIYQVPPSAGMGHDIYQVPPSMDTRSWEGTKPPAKVVVPTRVGQGYVYEAAQPEQDEY
  DIPRHLLAPGPQDIYDVPPVRGLLPSQYGQEVYDTPPMAVKGPNGRDPLLEVYDVPPSVEKGLPPSNHHA
  VYDVPPSVSKDVPDGPLLREETYDVPPAFAKAKPFDPARTPLVLAAPPPDSPPAEDVYDVPPPAPDLYDV
  PPGLRRPGPGTLYDVPRERVLPPEVADGGVVDSGVYAVPPPAEREAPAEGKRLSASSTGSTRSSQSASSL
  EVAGPGREPLELEVAVEALARLQQGVSATVAHLLDLAGSAGATGSWRSPSEPQEPLVQDLQAAVAAVQSA
  VHELLEFARSAVGNAAHTSDRALHAKLSRQLQKMEDVHQTLVAHGQALDAGRGGSGATLEDLDRLVACSR
  AVPEDAKQLASFLHGNASLLFRRTKATAPGPEGGGTLHPNPTDKTSSIQSRPLPSPPKFTSQDSPDGQYE
  NSEGGWMEDYDYVHLQGKEEFEKTQKELLEKGSITRQGKSQLELQQLKQFERLEQEVSRPIDHDLANWTP
  AQPLAPGRTGGLGPSDRQLLLFYLEQCEANLTTLTNAVDAFFTAVATNQPPKIFVAHSKFVILSAHKLVF
  IGDTLSRQAKAADVRSQVTHYSNLLCDLLRGIVATTKAAALQYPSPSAAQDMVERVKELGHSTQQFRRVL
  GQLAAA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001170714
• ORF Size: 2748 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001170714.3
• RefSeq ORF: 2751 bp
• Locus ID: 9564
• UniProt ID: P56945
• Cytogenetics: 16q23.1
• Protein Families: Druggable Genome
• Protein Pathways: Chemokine signaling pathway, Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton
• MW: 98.3 kDa
• Gene Summary: The protein encoded by this gene is a member of the Crk-associated substrate (CAS) family of scaffold proteins, characterized by the presence of multiple protein-protein interaction domains and many serine and tyrosine phosphorylation sites. The encoded protein contains a Src-homology 3 (SH3) domain, a proline-rich domain, a substrate domain which contains 15 repeat of the YxxP consensus phosphorylation motif for Src family kinases, a serine-rich domain, and a bipartite Src-binding domain, which can bind both SH2 and SH3 domains. This adaptor protein functions in multiple cellular pathways, including in cell motility, apoptosis and cell cycle control. Dysregulation of this gene can have a wide range of effects, affecting different pathways, including cardiac development, vascular smooth muscle cells, liver and kidney function, endothelial migration, and cancer. [provided by RefSeq, Sep 2017]