MAN1 (LEMD3) (NM_001167614) Human Tagged ORF Clone

CAT#: RC230581

LEMD3 (Myc-DDK-tagged)-Human LEM domain containing 3 (LEMD3), transcript variant 2

Product Images

Circular map for RC230581

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  Western blot validation of overexpression lysate (Cat# LY433581) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC230581 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified LEMD3 protein (Cat# TP330581). The protein was produced from HEK293T cells transfected with LEMD3 cDNA clone (Cat# RC230581) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MAN1
• Synonyms: MAN1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230581 representing NM_001167614
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGCAGCAGCTTCGGCGCCTCAGCAGCTCTCGGATGAGGAGCTTTTCTCTCAGCTCCGCCGTT
  ACGGCCTGTCTCCCGGACCAGTGACGGAGAGCACCCGCCCGGTCTACCTCAAGAAGCTGAAGAAGCTTCG
  AGAGGAAGAGCAGCAACAGCACCGGTCAGGGGGCCGCGGCAACAAGACGCGGAACAGTAATAACAATAAC
  ACGGCAGCCGCCACGGTCGCAGCCGCGGGACCAGCGGCGGCGGCGGCCGCGGGGATGGGGGTCCGGCCGG
  TCTCGGGCGACCTCTCCTACTTACGGACTCCTGGGGGCCTGTGCCGAATCTCGGCCTCTGGCCCAGAGAG
  CCTCCTGGGAGGGCCCGGGGGCGCCTCCGCCGCCCCCGCGGCTGGCAGCAAAGTGCTGCTGGGCTTCAGC
  TCGGACGAGTCGGACGTGGAGGCCAGTCCCCGGGACCAGGCCGGCGGCGGCGGGAGGAAAGACCGGGCTT
  CGCTCCAGTACCGCGGGCTCAAAGCGCCGCCGGCGCCCCTGGCCGCCAGCGAGGTGACTAACAGCAACTC
  TGCAGAGCGAAGGAAGCCCCACTCGTGGTGGGGGGCCAGGAGGCCGGCGGGCCCCGAGCTGCAGACCCCG
  CCGGGGAAAGATGGAGCAGTGGAGGACGAGGAAGGGGAGGGAGAGGACGGTGAGGAGAGGGACCCGGAGA
  CCGAGGAGCCGCTCTGGGCGAGCCGGACCGTGAATGGCAGCCGGCTTGTCCCCTACAGCTGCCGGGAAAA
  CTATTCGGACTCAGAGGAAGAGGACGACGACGACGTGGCCTCCAGCAGACAGGTATTAAAGGACGACTCC
  CTTTCCCGGCATCGGCCCAGACGAACCCATAGTAAGCCTCTCCCCCCGCTGACTGCTAAATCGGCCGGCG
  GCAGGCTGGAGACTTCAGTTCAGGGAGGGGGAGGACTCGCGATGAATGACAGGGCGGCGGCTGCCGGGAG
  TCTAGACAGGAGCCGAAACCTCGAAGAGGCGGCGGCCGCGGAGCAGGGAGGAGGGTGTGATCAAGTGGAC
  TCCAGCCCCGTTCCTAGATACCGTGTTAACGCTAAGAAACTGACCCCTCTCCTGCCCCCGCCACTTACTG
  ACATGGACTCAACCTTGGATTCGTCAACAGGCTCCCTTCTGAAAACCAATAATCATATTGGCGGTGGGGC
  CTTCAGTGTGGACTCCCCCAGGATTTATTCTAACAGTCTCCCTCCCAGTGCGGCGGTGGCCGCCTCTAGT
  TCACTCAGGATCAATCACGCCAATCATACGGGCTCCAATCATACCTACCTGAAAAACACATACAACAAAC
  CGAAGCTTTCCGAACCCGAAGAGGAACTTCTCCAGCAATTTAAACGGGAGGAGGTGTCCCCAACAGGGAG
  TTTCAGTGCCCACTACTTGTCGATGTTTCTCTTAACTGCTGCCTGCTTATTTTTCCTAATACTGGGACTG
  ACTTACCTAGGAATGAGAGGGACAGGAGTATCTGAGGATGGAGAACTCAGCAAAAACCCCTTTGGTGAAA
  CATTTGGAAAAATACAAGAAAGTGAAAAAACTCTTATGATGAACACATTATATAAGCTTCATGATCGATT
  GGCACAGCTTGCAGGAGATCATGAATGTGGCAGTTCTAGTCAAAGAACGCTTTCTGTTCAAGAGGCAGCT
  GCGTATTTAAAAGATTTAGGTCCTGAATATGAAGGTATATTTAACACTTCATTGCAGTGGATCTTAGAAA
  ATGGAAAAGATGTTGGAATAAGGTGTGTTGGTTTTGGCCCTGAGGAAGAATTGACAAATATAACTGATGT
  GCAGTTTTTACAGTCCACAAGACCACTGATGTCTTTTTGGTGTCGTTTTCGACGTGCTTTTGTTACTGTA
  ACTCACAGATTATTGTTGTTATGCTTAGGTGTAGTGATGGTTTGTGTCGTTCTGCGTTACATGAAATATC
  GATGGACAAAAGAAGAGGAGGAAACAAGGCAGATGTATGATATGGTGGTAAAGATTATAGATGTTTTACG
  AAGTCATAATGAAGCCTGCCAGGAAAACAAAGATTTACAACCTTACATGCCTATTCCACATGTACGCGAT
  TCCTTAATACAGCCTCATGACAGGAAAAAAATGAAGAAAGTCTGGGATAGAGCTGTTGACTTCCTTGCTG
  CTAATGAGTCTAGAGTTCGCACGGAAACACGAAGAATAGGTGGTGCAGATTTTCTGGTTTGGCGGTGGAT
  CCAGCCTTCTGCATCCTGTGACAAAATATTAGTTATACCTTCTAAAGTATGGCAAGGTCAAGCATTTCAT
  TTAGATAGAAGAAATTCACCACCAAATAGTTTGACACCGTGTCTAAAGATTCGGAATATGTTTGATCCCG
  TTATGGAAATAGGGGATCAGTGGCATTTGGCAATTCAAGAAGCAATTTTAGAAAAATGCAGTGATAATGA
  TGGCATTGTTCACATTGCAGTAGACAAAAATTCACGTGAGGGTTGTGTATATGTTAAATGTCTGTCTCCA
  GAATATGCTGGAAAGGCTTTTAAAGCATTGCATGGCTCTTGGTTTGATGGGAAATTGGTTACAGTAAAAT
  ATTTACGACTAGATAGATACCACCATCGCTTTCCCCAGGCTCTCACTTCCAACACTCCATTGAAGCCATC
  AAATAAACATATGAACTCCATGTCTCATCTTCGTCTTCGGACTGGCCTAACCAATTCTCAAGGAAGTTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230581 representing NM_001167614
  Red=Cloning site Green=Tags(s)
  
  MAAAAASAPQQLSDEELFSQLRRYGLSPGPVTESTRPVYLKKLKKLREEEQQQHRSGGRGNKTRNSNNNN
  TAAATVAAAGPAAAAAAGMGVRPVSGDLSYLRTPGGLCRISASGPESLLGGPGGASAAPAAGSKVLLGFS
  SDESDVEASPRDQAGGGGRKDRASLQYRGLKAPPAPLAASEVTNSNSAERRKPHSWWGARRPAGPELQTP
  PGKDGAVEDEEGEGEDGEERDPETEEPLWASRTVNGSRLVPYSCRENYSDSEEEDDDDVASSRQVLKDDS
  LSRHRPRRTHSKPLPPLTAKSAGGRLETSVQGGGGLAMNDRAAAAGSLDRSRNLEEAAAAEQGGGCDQVD
  SSPVPRYRVNAKKLTPLLPPPLTDMDSTLDSSTGSLLKTNNHIGGGAFSVDSPRIYSNSLPPSAAVAASS
  SLRINHANHTGSNHTYLKNTYNKPKLSEPEEELLQQFKREEVSPTGSFSAHYLSMFLLTAACLFFLILGL
  TYLGMRGTGVSEDGELSKNPFGETFGKIQESEKTLMMNTLYKLHDRLAQLAGDHECGSSSQRTLSVQEAA
  AYLKDLGPEYEGIFNTSLQWILENGKDVGIRCVGFGPEEELTNITDVQFLQSTRPLMSFWCRFRRAFVTV
  THRLLLLCLGVVMVCVVLRYMKYRWTKEEEETRQMYDMVVKIIDVLRSHNEACQENKDLQPYMPIPHVRD
  SLIQPHDRKKMKKVWDRAVDFLAANESRVRTETRRIGGADFLVWRWIQPSASCDKILVIPSKVWQGQAFH
  LDRRNSPPNSLTPCLKIRNMFDPVMEIGDQWHLAIQEAILEKCSDNDGIVHIAVDKNSREGCVYVKCLSP
  EYAGKAFKALHGSWFDGKLVTVKYLRLDRYHHRFPQALTSNTPLKPSNKHMNSMSHLRLRTGLTNSQGSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001167614
• ORF Size: 2730 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001167614.2
• RefSeq ORF: 2733 bp
• Locus ID: 23592
• UniProt ID: Q9Y2U8
• Cytogenetics: 12q14.3
• Protein Families: Transmembrane
• MW: 100.3 kDa
• Gene Summary: This locus encodes a LEM domain-containing protein. The encoded protein functions to antagonize transforming growth factor-beta signaling at the inner nuclear membrane. Two transcript variants encoding different isoforms have been found for this gene. Mutations in this gene have been associated with osteopoikilosis, Buschke-Ollendorff syndrome and melorheostosis.[provided by RefSeq, Nov 2009]