PCCA (NM_001178004) Human Tagged ORF Clone

CAT#: RC230422

PCCA (Myc-DDK-tagged)-Human propionyl CoA carboxylase, alpha polypeptide (PCCA), transcript variant 3

Product Images

Circular map for RC230422

• 

  Western blot validation of overexpression lysate (Cat# LY433422) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC230422 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PCCA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230422 representing NM_001178004
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGGTTCTGGGTCGGGACAGCACCGCTGGTCGCTGCCGGACGGCGTGGGCGGTGGCCGCCGCAGC
  AGCTGATGCTGAGCGCGGCGCTGCGGACCCTGAAGCATGTTCTGTACTATTCAAGACAGTGCTTAATGGT
  GTCCCGTAATCTTGGTTCAGTGGGATATGATCCTAATGAAAAAACTTTTGATAAAATTCTTGTTGCTAAT
  AGAGGAGAAATTGCATGTCGGGTTATTAGAACTTGCAAGAAGATGGGCATTAAGACAGTTGCCATCCACA
  GTGATGTTGATGCTAGTTCTGTTCATGTGAAAATGGCGGATGAGGCTGTCTGTGTTGGCCCAGCTCCCAC
  CAGTAAAAGCTACCTCAACATGGATGCCATCATGGAAGCCATTAAGAAAACCAGGGCCCAAGCTGTACAT
  CCAGGTTATGGATTCCTTTCAGAAAACAAAGAATTTGCCAGATGTTTGGCAGCAGAAGATGTCGTTTTCA
  TTGGACCTGACACACATGCTATTCAAGCCATGGGCGACAAGATTGAAAGCAAATTATTAGCTAAGAAAGC
  AGAGGTTAATACAATCCCTGGCTTTGATGGAGTAGTCAAGGATGCAGAAGAAGCTGTCAGAATTGCAAGG
  GAAATTGGCTACCCTGTCATGATCAAGGCCTCAGCAGGTGGTGGTGGGAAAGGCATGCGCATTGCTTGGG
  ATGATGAAGAGACCAGGGATGGTTTTAGATTGTCATCTCAAGAAGCTGCTTCTAGTTTTGGCGATGATAG
  ACTACTAATAGAAAAATTTATTGATAATCCTCGTCATATAGAAATCCAGGTTCTAGGTGATAAACATGGG
  AATGCTTTATGGCTTAATGAAAGAGAGTGCTCAATTCAGAGAAGAAATCAGAAGGTGGTGGAGGAAGCAC
  CAAGCATTTTTTTGGATGCGGAGACTCGAAGAGCGATGGGAGAACAAGCTGTAGCTCTTGCCAGAGCAGT
  AAAATATTCCTCTGCTGGGACCGTGGAGTTCCTTGTGGACTCTAAGAAGAATTTTTATTTCTTGGAAATG
  AATACAAGACTCCAGGTTGAGCATCCTGTCACAGAATGCATTACTGGCCTGGACCTAGTCCAGGAAATGA
  TCCGTGTTGCTAAGGGCTACCCTCTCAGGCACAAACAAGCTGATATTCGCATCAACGGCTGGGCAGTTGA
  ATGTCGGGTTTATGCTGAGGACCCCTACAAGTCTTTTGGTTTACCATCTATTGGGAGATTGTCTCAGTAC
  CAAGAACCGTTACATCTACCTGGTGTCCGAGTGGACAGTGGCATCCAACCAGGAAGTGATATTAGCATTT
  ATTATGATCCTATGATTTCAAAACTAATCACATATGGCTCTGATAGAACTGAGGCACTGAAGAGAATGGC
  AGATGCACTGGATAACTATGTTATTCGAGGTGTTACACATAATATTGCATTACTTCGAGAGGTGATAATC
  AACTCACGCTTTGTAAAAGGAGACATCAGCACTAAATTTCTCTCCGATGTGTATCCTGATGGCTTCAAAG
  GACACATGCTAACCAAGAGTGAGAAGAACCAGTTATTGGCAATAGCATCATCATTGTTTGTGGCATTCCA
  GTTAAGAGCACAACATTTTCAAGAAAATTCAAGAATGCCTGTTATTAAACCAGACATAGCCAACTGGGAG
  CTCTCAGTAAAATTGCATGATAAAGTTCATACCGTAGTAGCATCAAACAATGGGTCAGTGTTCTCGGTGG
  AAGTTGATGGGTCGAAACTAAATGTGACCAGCACGTGGAACCTGGCTTCGCCCTTATTGTCTGTCAGCGT
  TGATGGCACTCAGAGGACTGTCCAGTGTCTTTCTCGAGAAGCAGGTGGAAACATGAGCATTCAGTTTCTT
  GGTACAGTGGTAGCAGAAGGTCAAGAAATTTGTGTGATTGAAGCCATGAAAATGCAGAATAGTATGACAG
  CTGGGAAAACTGGCACGGTGAAATCTGTGCACTGTCAAGCTGGAGACACAGTTGGAGAAGGGGATCTGCT
  CGTGGAGCTGGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230422 representing NM_001178004
  Red=Cloning site Green=Tags(s)
  
  MAGFWVGTAPLVAAGRRGRWPPQQLMLSAALRTLKHVLYYSRQCLMVSRNLGSVGYDPNEKTFDKILVAN
  RGEIACRVIRTCKKMGIKTVAIHSDVDASSVHVKMADEAVCVGPAPTSKSYLNMDAIMEAIKKTRAQAVH
  PGYGFLSENKEFARCLAAEDVVFIGPDTHAIQAMGDKIESKLLAKKAEVNTIPGFDGVVKDAEEAVRIAR
  EIGYPVMIKASAGGGGKGMRIAWDDEETRDGFRLSSQEAASSFGDDRLLIEKFIDNPRHIEIQVLGDKHG
  NALWLNERECSIQRRNQKVVEEAPSIFLDAETRRAMGEQAVALARAVKYSSAGTVEFLVDSKKNFYFLEM
  NTRLQVEHPVTECITGLDLVQEMIRVAKGYPLRHKQADIRINGWAVECRVYAEDPYKSFGLPSIGRLSQY
  QEPLHLPGVRVDSGIQPGSDISIYYDPMISKLITYGSDRTEALKRMADALDNYVIRGVTHNIALLREVII
  NSRFVKGDISTKFLSDVYPDGFKGHMLTKSEKNQLLAIASSLFVAFQLRAQHFQENSRMPVIKPDIANWE
  LSVKLHDKVHTVVASNNGSVFSVEVDGSKLNVTSTWNLASPLLSVSVDGTQRTVQCLSREAGGNMSIQFL
  GTVVAEGQEICVIEAMKMQNSMTAGKTGTVKSVHCQAGDTVGEGDLLVELE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001178004
• ORF Size: 2043 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001178004.1, NP_001171475.1
• RefSeq ORF: 2046 bp
• Locus ID: 5095
• UniProt ID: P05165
• Cytogenetics: 13q32.3
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Propanoate metabolism, Valine, leucine and isoleucine degradation
• MW: 75.5 kDa
• Gene Summary: The protein encoded by this gene is the alpha subunit of the heterodimeric mitochondrial enzyme Propionyl-CoA carboxylase. PCCA encodes the biotin-binding region of this enzyme. Mutations in either PCCA or PCCB (encoding the beta subunit) lead to an enzyme deficiency resulting in propionic acidemia. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2010]