PRMT7 (NM_001184824) Human Tagged ORF Clone

CAT#: RC230386

PRMT7 (Myc-DDK-tagged)-Human protein arginine methyltransferase 7 (PRMT7), transcript variant 2

Product Images

Circular map for RC230386

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PRMT7
• Synonyms: SBIDDS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230386 representing NM_001184824
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGATCTTCTGCAGTCGGGCCAATCCGACCACGGGGTCTGTGGAGTGGCTGGAGGAGGATGAACACT
  ATGATTACCACCAGGAGATTGCAAGGTCATCTTATGCAGATATGCTACATGACAAAGACAGAGTTTTCAA
  GCCTATGGCTGATGCTGCTGTGAAGATTGTGGAGAAAAATGGCTTTAGTGATAAGATTAAGGTTATCAAC
  AAGCATTCCACCGAGGTGACTGTAGGTCCAGAGGGTGACATGCCATGCCGTGCCAACATCCTGGTCACAG
  AGTTGTTTGACACAGAGCTGATCGGGGAGGGGGCGCTGCCCTCCTATGAGCACGCACACAGGCATCTCGT
  GGAGGAAAATTGTGAGGCCGTGCCCCACAGAGCCACCGTCTATGCACAGCTGGTGGAGTCCGGGAGGATG
  TGGTCGTGGAACAAGCTATTTCCCATCCACGTGCAGACCAGCCTCGGAGAGCAGGTCATCGTCCCTCCCG
  TTGACGTGGAGAGCTGCCCTGGCGCACCCTCTGTCTGTGACATTCAGCTGAACCAGGTGTCACCAGCCGA
  CTTTACAGTCCTCAGCGATGTGCTGCCCATGTTCAGCATAGACTTCAGCAAGCAAGTCAGTAGCTCAGCA
  GCCTGCCATAGCAGGCGGTTTGAACCTCTGACATCTGGCCGAGCTCAGGTGGTTCTCTCGTGGTGGGACA
  TTGAAATGGACCCTGAGGGGAAGATCAAGTGCACCATGGCCCCCTTCTGGGCACACTCAGACCCAGAGGA
  GATGCAGTGGCGGGACCACTGGATGCAGTGTGTGTACTTCCTGCCACAAGAGGAGCCTGTGGTGCAGGGC
  TCAGCGCTCTATCTGGTAGCCCACCACGATGACTACTGCGTATGGTACAGCCTGCAGAGGACCAGCCCTG
  AAAAGAATGAGAGAGTCCGCCAGATGCGCCCCGTGTGTGACTGCCAGGCTCACCTGCTCTGGAACCGGCC
  TCGGTTTGGAGAGATCAATGACCAGGACAGAACTGATCGATACGTCCAGGCTCTGAGGACCGTGCTGAAG
  CCAGACAGCGTGTGCCTGTGTGTCAGCGATGGCAGCCTGCTCTCCGTGCTGGCCCATCACCTGGGGGTGG
  AGCAGGTGTTTACAGTCGAGAGTTCAGCAGCTTCTCACAAACTGTTGAGAAAAATCTTCAAGGCTAACCA
  CTTGGAAGATAAAATTAACATCATAGAGAAACGGCCGGAATTATTAACAAATGAGGACCTACAGGGCAGA
  AAGGTCTCTCTCCTCCTGGGCGAGCCGTTCTTCACTACCAGCCTGCTGCCGTGGCACAACCTCTACTTCT
  GGTACGTGCGGACCGCTGTGGACCAGCACCTGGGGCCAGGTGCCATGGTGATGCCCCAGGCAGCCTCGCT
  GCACGCTGTGGTTGTGGAGTTCAGGGACCTGTGGCGGATCCGGAGCCCCTGTGGTGACTGCGAAGGCTTC
  GACGTGCACATCATGGACGACATGATTAAGCGTGCCCTGGACTTCAGGGAGAGCAGGGAAGCTGAGCCCC
  ACCCGCTGTGGGAGTACCCATGCCGCAGCCTCTCCGAGCCCTGGCAGATCCTGACCTTTGACTTCCAGCA
  GCCGGTGCCCCTGCAGCCCCTGTGTGCCGAGGGCACCGTGGAGCTCAGAAGGCCCGGGCAGAGCCACGCA
  GCGGTGCTATGGATGGAGTACCACCTGACCCCGGAGTGCACGCTCAGCACTGGCCTCCTGGAGCCTGCAG
  ACCCCGAGGGGGGCTGCTGCTGGAACCCCCACTGCAAGCAGGCCGTCTACTTCTTCAGCCCTGCCCCAGA
  TCCCAGAGCACTGCTGGGTGGCCCACGGACTGTCAGCTATGCAGTGGAGTTTCACCCCGACACAGGCGAC
  ATCATCATGGAGTTCAGGCATGCAGATACCCCAGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230386 representing NM_001184824
  Red=Cloning site Green=Tags(s)
  
  MKIFCSRANPTTGSVEWLEEDEHYDYHQEIARSSYADMLHDKDRVFKPMADAAVKIVEKNGFSDKIKVIN
  KHSTEVTVGPEGDMPCRANILVTELFDTELIGEGALPSYEHAHRHLVEENCEAVPHRATVYAQLVESGRM
  WSWNKLFPIHVQTSLGEQVIVPPVDVESCPGAPSVCDIQLNQVSPADFTVLSDVLPMFSIDFSKQVSSSA
  ACHSRRFEPLTSGRAQVVLSWWDIEMDPEGKIKCTMAPFWAHSDPEEMQWRDHWMQCVYFLPQEEPVVQG
  SALYLVAHHDDYCVWYSLQRTSPEKNERVRQMRPVCDCQAHLLWNRPRFGEINDQDRTDRYVQALRTVLK
  PDSVCLCVSDGSLLSVLAHHLGVEQVFTVESSAASHKLLRKIFKANHLEDKINIIEKRPELLTNEDLQGR
  KVSLLLGEPFFTTSLLPWHNLYFWYVRTAVDQHLGPGAMVMPQAASLHAVVVEFRDLWRIRSPCGDCEGF
  DVHIMDDMIKRALDFRESREAEPHPLWEYPCRSLSEPWQILTFDFQQPVPLQPLCAEGTVELRRPGQSHA
  AVLWMEYHLTPECTLSTGLLEPADPEGGCCWNPHCKQAVYFFSPAPDPRALLGGPRTVSYAVEFHPDTGD
  IIMEFRHADTPD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001184824
• ORF Size: 1926 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001184824.3
• RefSeq ORF: 1929 bp
• Locus ID: 54496
• UniProt ID: Q9NVM4
• Cytogenetics: 16q22.1
• Protein Families: Druggable Genome
• MW: 73.6 kDa
• Gene Summary: This gene encodes a member of the protein arginine N-methyltransferase family of proteins. The encoded enzyme transfers single methyl groups to arginine residues to generate monomethylarginines on histone proteins as well as other protein substrates. This enzyme plays a role in a wide range of biological processes, including neuronal differentiation, male germ line imprinting, small nuclear ribonucleoprotein biogenesis, and regulation of the Wnt signaling pathway. Mutations in this gene underlie multiple related syndromes in human patients characterized by intellectual disability, short stature and other features. The encoded protein may promote breast cancer cell invasion and metastasis in human patients. [provided by RefSeq, May 2017]