SRPR alpha (SRPRA) (NM_001177842) Human Tagged ORF Clone

CAT#: RC230343

SRPR (Myc-DDK-tagged)-Human signal recognition particle receptor (docking protein) (SRPR), transcript variant 2

Product Images

Circular map for RC230343

• 

  Coomassie blue staining of purified SRPRA protein (Cat# TP330343). The protein was produced from HEK293T cells transfected with SRPRA cDNA clone (Cat# RC230343) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SRPR alpha
• Synonyms: DP; Sralpha; SRPR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230343 representing NM_001177842
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCGACTTCTTCACCATTTTCTCCAAGGGCGGGCTTGTGCTCTGGTGCTTCCAGGGCGTTAGCGACT
  CATGCACCGGACCCGTTAACGCGTTGATTCGTTCCGTGCTGCTGCAGGTTGGTTTTCAGAAGATCCTGAC
  ACTGACATATGTAGACAAATTGATAGATGACGTGCATCGGCTGTTTCGGGACAAGTACCGCACAGAGATC
  CAACAGCAAAGTGCTTTAAGTTTATTAAATGGCACTTTTGATTTCCAAAATGACTTCCTGCGGCTCCTTC
  GTGAAGCAGAGGAGAGCAGTAAGATCCGTGCTCCCACTACCATGAAGAAATTTGAAGATTCTGAAAAGGC
  CAAGAAACCTGTGAGGTCCATGATTGAGACACGGGGGGAAAAGCCCAAGGAAAAAGCAAAGAATAGCAAA
  AAAAAGGGGGCCAAGAAGGAAGGTTCTGATGGTCCTTTGGCTACCAGCAAACCAGTCCCTGCAGAAAAGT
  CAGGTCTTCCAGTGGGTCCTGAGAACGGAGTAGAACTTTCCAAAGAGGAGCTGATCCGCAGGAAGCGCGA
  GGAGTTCATTCAGAAGCATGGGAGGGGTATGGAGAAGTCCAACAAGTCCACGAAGTCAGATGCTCCAAAG
  GAGAAGGGCAAAAAAGCACCCCGGGTGTGGGAACTGGGTGGCTGTGCTAACAAAGAAGTGTTGGATTACA
  GTACTCCCACCACCAATGGAACCCCTGAGGCTGCCTTGTCTGAGGACATCAACCTGATTCGAGGGACTGG
  GTCTGGGGGGCAGCTTCAGGATCTGGACTGCAGCAGCTCTGATGACGAAGGGGCTGCTCAAAACTCTACC
  AAACCTAGTGCGACCAAGGGAACACTGGGTGGCATGTTTGGTATGCTGAAGGGCCTTGTGGGTTCAAAGA
  GCTTGAGTCGTGAAGACATGGAATCTGTGCTGGACAAGATGCGTGATCATCTCATTGCTAAGAACGTGGC
  TGCAGACATTGCCGTCCAGCTCTGTGAATCTGTTGCCAACAAGTTGGAAGGGAAGGTGATGGGGACGTTC
  AGCACGGTGACTTCCACAGTAAAGCAAGCCCTACAGGAGTCCCTGGTGCAGATTCTGCAGCCACAGCGTC
  GTGTAGACATGCTCCGGGACATCATGGATGCCCAGCGTCGCCAGCGCCCTTATGTCGTCACCTTCTGCGG
  CGTTAATGGAGTGGGGAAATCTACTAATCTTGCCAAGATTTCCTTCTGGTTGTTAGAGAATGGCTTCAGT
  GTCCTCATTGCTGCCTGTGATACATTTCGTGCTGGGGCCGTGGAGCAGCTGCGTACACACACCCGGCGTT
  TGAGTGCCCTACACCCTCCAGAGAAGCATGGTGGCCGCACCATGGTGCAGTTGTTTGAAAAGGGCTATGG
  CAAGGATGCTGCTGGCATTGCCATGGAAGCCATTGCTTTTGCACGTAACCAAGGCTTTGACGTGGTGCTG
  GTGGACACGGCAGGCCGCATGCAAGACAATGCCCCTCTGATGACTGCCCTGGCCAAACTCATTACTGTCA
  ATACACCTGATTTGGTGCTGTTTGTAGGAGAAGCCTTAGTAGGCAATGAAGCCGTGGACCAGCTGGTCAA
  GTTCAACAGAGCCTTGGCTGACCATTCTATGGCTCAGACACCTCGGCTCATTGATGGCATTGTTCTTACC
  AAATTTGATACCATTGATGACAAGGTGGGAGCTGCTATTTCTATGACGTACATCACAAGCAAACCCATCG
  TCTTTGTGGGCACCGGCCAGACCTACTGTGACCTACGCAGCCTCAATGCCAAGGCTGTGGTGGCTGCCCT
  CATGAAGGCT
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230343 representing NM_001177842
  Red=Cloning site Green=Tags(s)
  
  MLDFFTIFSKGGLVLWCFQGVSDSCTGPVNALIRSVLLQVGFQKILTLTYVDKLIDDVHRLFRDKYRTEI
  QQQSALSLLNGTFDFQNDFLRLLREAEESSKIRAPTTMKKFEDSEKAKKPVRSMIETRGEKPKEKAKNSK
  KKGAKKEGSDGPLATSKPVPAEKSGLPVGPENGVELSKEELIRRKREEFIQKHGRGMEKSNKSTKSDAPK
  EKGKKAPRVWELGGCANKEVLDYSTPTTNGTPEAALSEDINLIRGTGSGGQLQDLDCSSSDDEGAAQNST
  KPSATKGTLGGMFGMLKGLVGSKSLSREDMESVLDKMRDHLIAKNVAADIAVQLCESVANKLEGKVMGTF
  STVTSTVKQALQESLVQILQPQRRVDMLRDIMDAQRRQRPYVVTFCGVNGVGKSTNLAKISFWLLENGFS
  VLIAACDTFRAGAVEQLRTHTRRLSALHPPEKHGGRTMVQLFEKGYGKDAAGIAMEAIAFARNQGFDVVL
  VDTAGRMQDNAPLMTALAKLITVNTPDLVLFVGEALVGNEAVDQLVKFNRALADHSMAQTPRLIDGIVLT
  KFDTIDDKVGAAISMTYITSKPIVFVGTGQTYCDLRSLNAKAVVAALMKA
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001177842
• ORF Size: 1830 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001177842.1, NP_001171313.1
• RefSeq ORF: 1833 bp
• Locus ID: 6734
• UniProt ID: P08240
• Cytogenetics: 11q24.2
• Protein Families: Druggable Genome
• Protein Pathways: Protein export
• MW: 67 kDa
• Gene Summary: The gene encodes a subunit of the endoplasmic reticulum signal recognition particle receptor that, in conjunction with the signal recognition particle, is involved in the targeting and translocation of signal sequence tagged secretory and membrane proteins across the endoplasmic reticulum. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010]