FCAMR (NM_001170631) Human Tagged ORF Clone

CAT#: RC230305

FCAMR (Myc-DDK-tagged)-Human Fc receptor, IgA, IgM, high affinity (FCAMR), transcript variant 3

Product Images

Circular map for RC230305

• 

  Western blot validation of overexpression lysate (Cat# LY433305) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC230305 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FCAMR
• Synonyms: CD351; FCA/MR; FKSG87
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230305 representing NM_001170631
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGGAGAGGCCACAGTGAAGCCTGGAGAACAAAAGGAAGTGGTGAGGAGAGGAAGAGAAGTGGACT
  ACTCCAGGCTCATTGCTGGCACTTTACCACAATCTCACGTCACCAGCAGGAGGGCGGGATGGAAAATGCC
  CCTCTTCCTCATACTGTGCCTGCTACAAGGTTCTTCTTTCGCCCTTCCACAAAAAAGACCCCATCCGAGA
  TGGCTGTGGGAGGGCTCTCTCCCCTCCAGGACCCATCTCCGGGCCATGGGAACACTCAGGCCTTCCTCGC
  CCCTCTGCTGGCGGGAGGAGAGCTCCTTTGCAGCTCCAAATTCATTGAAGGGCTCAAGGCTGGTGTCAGG
  GGAGCCTGGAGGAGCTGTCACCATCCAGTGCCATTATGCCCCCTCATCTGTCAACAGGCACCAGAGGAAG
  TACTGGTGCCGTCTGGGGCCCCCAAGATGGATCTGCCAGACCATTGTGTCCACCAACCAGTATACTCACC
  ATCGCTATCGTGACCGTGTGGCCCTCACAGACTTTCCACAGAGAGGCTTGTTTGTGGTGAGGCTGTCCCA
  ACTGTCCCCGGATGACATCGGATGCTACCTCTGCGGCATTGGAAGTGAAAACAACATGCTGTTCTTAAGC
  ATGAATCTGACCATCTCTGCAGGTCCCGCCAGCACCCTCCCCACAGCCACTCCAGCTGCTGGGGAGCTCA
  CCATGAGATCCTATGGAACAGCGTCTCCAGTGGCCAACAGATGGACCCCAGGAACCACCCAGACCTTAGG
  ACAGGGGACAGCATGGGACACAGTTGCTTCCACTCCAGGAACCAGCAAGACTACAGCTTCAGCTGAGGGA
  AGACGAACCCCAGGAGCAACCAGGCCAGCAGCTCCAGGGACAGGCAGCTGGGCAGAGGGTTCTGTCAAAG
  CACCTGCTCCGATTCCAGAGAGTCCACCTTCAAAGAGCAGAAGCATGTCCAATACAACAGAAGGTGTTTG
  GGAGGGCACCAGAAGCTCGGTGACAAACAGGGCTAGAGCCAGCAAGGACAGGAGGGAGATGACAACTACC
  AAGGCTGATAGGCCAAGGGAGGACATAGAGGGGGTCAGGATAGCTCTTGATGCAGCCAAAAAGGTCCTAG
  GAACCATTGGGCCACCAGCTCTGGTCTCAGAAACTTTGGCCTGGGAAATCCTCCCACAAGCAACGCCAGT
  TTCTAAGCAACAATCTCAGGGTTCCATTGGAGAAACAACTCCAGCTGCAGGCATGTGGACCTTGGGAACT
  CCAGCTGCAGATGTGTGGATCTTGGGAACTCCAGCTGCAGATGTGTGGACCAGCATGGAGGCAGCATCTG
  GGGAAGGAAGCGCTGCAGGGGACCTAGATGCTGCCACTGGAGACAGAGGTCCCCAAGCAACACTGAGCCA
  GACCCCGGCAGTAGGACCCTGGGGACCCCCTGGCAAGGAGTCCTCCGTGAAGCGTACTTTTCCAGAAGAT
  GAAAGCAGCTCTCGGACCCTGGCTCCTGTCTCTACCATGCTGGCCCTGTTTATGCTTATGGCTCTGGTTC
  TATTGCAAAGGAAGCTCTGGAGAAGGAGGACCTCTCAGGAGGCAGAAAGGGTCACCTTAATTCAGATGAC
  ACATTTTCTGGAAGTGAACCCCCAAGCAGACCAGCTGCCCCATGTGGAAAGAAAGATGCTCCAGGATGAC
  TCTCTTCCTGCTGGGGCCAGCCTGACTGCCCCAGAGAGAAATCCAGGACCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230305 representing NM_001170631
  Red=Cloning site Green=Tags(s)
  
  MDGEATVKPGEQKEVVRRGREVDYSRLIAGTLPQSHVTSRRAGWKMPLFLILCLLQGSSFALPQKRPHPR
  WLWEGSLPSRTHLRAMGTLRPSSPLCWREESSFAAPNSLKGSRLVSGEPGGAVTIQCHYAPSSVNRHQRK
  YWCRLGPPRWICQTIVSTNQYTHHRYRDRVALTDFPQRGLFVVRLSQLSPDDIGCYLCGIGSENNMLFLS
  MNLTISAGPASTLPTATPAAGELTMRSYGTASPVANRWTPGTTQTLGQGTAWDTVASTPGTSKTTASAEG
  RRTPGATRPAAPGTGSWAEGSVKAPAPIPESPPSKSRSMSNTTEGVWEGTRSSVTNRARASKDRREMTTT
  KADRPREDIEGVRIALDAAKKVLGTIGPPALVSETLAWEILPQATPVSKQQSQGSIGETTPAAGMWTLGT
  PAADVWILGTPAADVWTSMEAASGEGSAAGDLDAATGDRGPQATLSQTPAVGPWGPPGKESSVKRTFPED
  ESSSRTLAPVSTMLALFMLMALVLLQRKLWRRRTSQEAERVTLIQMTHFLEVNPQADQLPHVERKMLQDD
  SLPAGASLTAPERNPGP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001170631
• ORF Size: 1731 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001170631.2
• RefSeq ORF: 1734 bp
• Locus ID: 83953
• Cytogenetics: 1q32.1
• Protein Families: Druggable Genome, Transmembrane
• MW: 62.6 kDa
• Gene Summary: Functions as a receptor for the Fc fragment of IgA and IgM. Binds IgA and IgM with high affinity and mediates their endocytosis. May function in the immune response to microbes mediated by IgA and IgM.[UniProtKB/Swiss-Prot Function]