TUBAL3 (NM_001171864) Human Tagged ORF Clone

CAT#: RC230015

TUBAL3 (Myc-DDK-tagged)-Human tubulin, alpha-like 3 (TUBAL3), transcript variant 2

Product Images

Circular map for RC230015

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TUBAL3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230015 representing NM_001171864
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCAGCTGGAAAATGCAAAAATGGAGCACACAAATGCATCTTTCGATACCTTCTTCTGTGAGACAA
  GAGCTGGGAAGCATGTGCCTAGAGCACTCTTCGTGGACTTGGAGCCAACTGTTATAGATGGGATCCGGAC
  GGGCCAGCACCGTTCACTCTTCCACCCCGAGCAGCTCCTTAGCGGAAAGGAGGATGCTGCTAACAATTAC
  GCGCGAGGCCGTTACTCTGTGGGGTCGGAGGTCATCGACCTTGTGCTGGAGAGGACCCGGAAGCTGGCAG
  AACAGTGTGGTGGACTTCAGGGATTTTTGATTTTCCGAAGCTTTGGAGGAGGCACTGGTTCAGGGTTTAC
  GTCTCTCTTAATGGAGAGGCTCACAGGAGAATATAGCAGAAAGACTAAGCTGGAGTTCTCGGTCTACCCA
  GCCCCCAGGATCTCCACTGCTGTGGTAGAGCCTTATAACTCTGTCCTCACCACCCACTCCACCACAGAGC
  ACACGGACTGTACCTTCATGGTGGACAACGAGGCCGTCTATGATATATGCCATCGTAAACTCGGTGTTGA
  ATGCCCCTCTCATGCCAGCATCAATAGATTGGTGGTTCAGGTGGTATCTTCCATCACTGCCTCCCTCCGG
  TTTGAAGGGCCCTTGAATGTAGACCTAATTGAATTCCAGACCAACCTGGTACCTTATCCGAGAATACATT
  TCCCCATGACAGCCTTCGCCCCCATCGTCTCTGCTGACAAAGCCTACCATGAGCAGTTCTCTGTGTCAGA
  CATCACCACTGCCTGCTTTGAGTCCTCCAACCAGCTGGTCAAGTGTGATCCTCGGCTTGGGAAGTACATG
  GCCTGCTGCCTACTCTATAGAGGGGATGTGGTCCCCAAGGAAGTGAATGCAGCAATCGCAGCCACGAAGT
  CGAGGCACTCTGTTCAGTTTGTAGATTGGTGTCCAACTGGTTTCAAGGTGGGCATCAACAATCGGCCGCC
  CACGGTGATGCCGGGTGGGGACCTGGCCAAAGTCCACCGGTCCATCTGCATGCTGAGCAACACCACGGCG
  ATTGTGGAGGCCTGGGCCCGCCTGGACCACAAGTTTGACCTCATGTACGCCAAGAGAGCATTTCTGCACT
  GGTACCTCAGAGAAGGCATGGAAGAAGCAGAGTTCTTGGAGGCCAGGGAAGATCTGGCAGCCCTGGAGAG
  GGACTATGAGGAAGTGGCGCAAAGTTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230015 representing NM_001171864
  Red=Cloning site Green=Tags(s)
  
  MDQLENAKMEHTNASFDTFFCETRAGKHVPRALFVDLEPTVIDGIRTGQHRSLFHPEQLLSGKEDAANNY
  ARGRYSVGSEVIDLVLERTRKLAEQCGGLQGFLIFRSFGGGTGSGFTSLLMERLTGEYSRKTKLEFSVYP
  APRISTAVVEPYNSVLTTHSTTEHTDCTFMVDNEAVYDICHRKLGVECPSHASINRLVVQVVSSITASLR
  FEGPLNVDLIEFQTNLVPYPRIHFPMTAFAPIVSADKAYHEQFSVSDITTACFESSNQLVKCDPRLGKYM
  ACCLLYRGDVVPKEVNAAIAATKSRHSVQFVDWCPTGFKVGINNRPPTVMPGGDLAKVHRSICMLSNTTA
  IVEAWARLDHKFDLMYAKRAFLHWYLREGMEEAEFLEAREDLAALERDYEEVAQSF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001171864
• ORF Size: 1218 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001171864.2
• RefSeq ORF: 1221 bp
• Locus ID: 79861
• UniProt ID: A6NHL2
• Cytogenetics: 10p15.1
• Protein Families: Druggable Genome
• Protein Pathways: Gap junction, Pathogenic Escherichia coli infection
• MW: 46 kDa
• Gene Summary: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity).[UniProtKB/Swiss-Prot Function]