FCRLM1 (FCRLA) (NM_001184866) Human Tagged ORF Clone

CAT#: RC229969

FCRLA (Myc-DDK-tagged)-Human Fc receptor-like A (FCRLA), transcript variant 1

Product Images

Circular map for RC229969

• 

  Western blot validation of overexpression lysate (Cat# LY432969) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC229969 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FCRLM1
• Synonyms: FCRL; FCRL1; FCRLb; FCRLc1; FCRLc2; FCRLd; FCRLe; FCRLM1; FCRLX; FCRX; FREB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC229969 representing NM_001184866
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGAAGAAAATCAGTGTTGGGGTTGCAGGAGACCTAAACACAGTCACCATGAAGCTGGGCTGTGTCC
  TCATGGCCTGGGCCCTCTACCTTTCCCTTGGTGTGCTCTGGGTGGCCCAGATGCTACTGGCAGCTGGATG
  TCATGCCGCTGCCAGTTTTGAGACGCTGCAGTGTGAGGGACCTGTCTGCACTGAGGAGAGCAGCTGCCAC
  ACGGAGGATGACTTGACTGATGCAAGGGAAGCTGGCTTCCAGGTCAAGGCCTACACTTTCAGTGAACCCT
  TCCACCTGATTGTGTCCTATGACTGGCTGATCCTCCAAGGTCCAGCCAAGCCAGTTTTTGAAGGGGACCT
  GCTGGTTCTGCGCTGCCAGGCCTGGCAAGACTGGCCACTGACTCAGGTGACCTTCTACCGAGATGGCTCA
  GCTCTGGGTCCCCCCGGGCCTAACAGGGAATTCTCCATCACCGTGGTACAAAAGGCAGACAGCGGGCACT
  ACCACTGCAGTGGCATCTTCCAGAGCCCTGGTCCTGGGATCCCAGAAACAGCATCTGTTGTGGCTATCAC
  AGTCCAAGAACTGTTTCCAGCGCCAATTCTCAGAGCTGTACCCTCAGCTGAACCCCAAGCAGGAAGCCCC
  ATGACCCTGAGTTGTCAGACAAAGTTGCCCCTGCAGAGGTCAGCTGCCCGCCTCCTCTTCTCCTTCTACA
  AGGATGGAAGGATAGTGCAAAGCAGGGGGCTCTCCTCAGAATTCCAGATCCCCACAGCTTCAGAAGATCA
  CTCCGGGTCATACTGGTGTGAGGCAGCCACTGAGGACAACCAAGTTTGGAAACAGAGCCCCCAGCTAGAG
  ATCAGAGTGCAGGGTGCTTCCAGCTCTGCTGCACCTCCCACATTGAATCCAGCTCCTCAGAAATCAGCTG
  CTCCAGGAACTGCTCCTGAGGAGGCCCCTGGGCCTCTGCCTCCGCCGCCAACCCCATCTTCTGAGGATCC
  AGGCTTTTCTTCTCCTCTGGGGATGCCAGATCCTCATCTGTATCACCAGATGGGCCTTCTTCTCAAACAC
  ATGCAGGATGTGAGAGTCCTCCTCGGTCACCTGCTCATGGAGTTGAGGGAATTATCTGGCCACCGGAAGC
  CTGGGACCACAAAGGCTACTGCTGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC229969 representing NM_001184866
  Red=Cloning site Green=Tags(s)
  
  MLKKISVGVAGDLNTVTMKLGCVLMAWALYLSLGVLWVAQMLLAAGCHAAASFETLQCEGPVCTEESSCH
  TEDDLTDAREAGFQVKAYTFSEPFHLIVSYDWLILQGPAKPVFEGDLLVLRCQAWQDWPLTQVTFYRDGS
  ALGPPGPNREFSITVVQKADSGHYHCSGIFQSPGPGIPETASVVAITVQELFPAPILRAVPSAEPQAGSP
  MTLSCQTKLPLQRSAARLLFSFYKDGRIVQSRGLSSEFQIPTASEDHSGSYWCEAATEDNQVWKQSPQLE
  IRVQGASSSAAPPTLNPAPQKSAAPGTAPEEAPGPLPPPPTPSSEDPGFSSPLGMPDPHLYHQMGLLLKH
  MQDVRVLLGHLLMELRELSGHRKPGTTKATAE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001184866
• ORF Size: 1146 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001184866.1, NP_001171795.1
• RefSeq ORF: 1098 bp
• Locus ID: 84824
• UniProt ID: Q7L513
• Cytogenetics: 1q23.3
• Protein Families: Secreted Protein, Transmembrane
• MW: 41.6 kDa
• Gene Summary: This gene encodes a protein similar to receptors for the Fc fragment of gamma immunoglobulin (IgG). These receptors, referred to as FCGRs, mediate the destruction of IgG-coated antigens and of cells induced by antibodies. This encoded protein is selectively expressed in B cells, and may be involved in their development. This protein may also be involved in the development of lymphomas. Multiple alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. [provided by RefSeq, Aug 2011]