ARL13B (NM_001174151) Human Tagged ORF Clone

CAT#: RC229892

ARL13B (Myc-DDK-tagged)-Human ADP-ribosylation factor-like 13B (ARL13B), transcript variant 4

Product Images

Circular map for RC229892

• 

  Western blot validation of overexpression lysate (Cat# LY432892) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC229892 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ARL13B
• Synonyms: ARL2L1; JBTS8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC229892 representing NM_001174151
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGAGACAAAAGAGGCTATGTCAGAAATGCTAAGACATCCTAGGATATCGGGAAAGCCTATATTGG
  TGTTGGCAAATAAACAAGATAAAGAAGGAGCTTTAGGAGAAGCTGATGTCATTGAATGTCTATCTCTGGA
  AAAATTGGTCAATGAGCACAAGTGCCTGTGTCAGATAGAACCATGTTCAGCAATCTCGGGGTATGGAAAG
  AAAATTGACAAGTCCATTAAAAAAGGCCTTTATTGGCTGCTACATGTTATTGCAAGAGACTTTGATGCCT
  TAAATGAACGCATCCAAAAAGAGACAACAGAGCAGCGTGCTCTTGAGGAACAAGAGAAACAAGAAAGAGC
  TGAACGAGTGCGAAAATTACGAGAAGAAAGAAAACAAAATGAACAGGAGCAGGCTGAACTCGATGGAACC
  AGTGGTCTGGCTGAGTTGGACCCAGAACCAACGAATCCTTTCCAGCCAATAGCATCTGTAATCATTGAGA
  ATGAAGGAAAACTTGAAAGAGAGAAAAAAAACCAAAAAATGGAGAAAGACAGTGATGGCTGCCACCTGAA
  ACATAAAATGGAGCATGAGCAAATAGAGACACAAGGCCAGGTTAATCACAATGGCCAAAAAAATAATGAA
  TTTGGACTAGTAGAAAATTATAAGGAGGCATTAACACAGCAGTTAAAGAATGAAGATGAGACAGACCGGC
  CATCATTGGAATCAGCTAATGGTAAAAAGAAAACTAAGAAACTAAGAATGAAAAGGAACCACCGGGTAGA
  ACCACTTAATATAGATGACTGTGCTCCTGAGAGTCCAACGCCACCCCCACCCCCTCCTCCTGTTGGCTGG
  GGAACCCCTAAAGTCACTAGACTTCCAAAACTTGAGCCTCTTGGTGAAACACATCATAATGATTTCTATA
  GGAAGCCACTGCCTCCCCTGGCTGTGCCACAGCGACCTAACAGTGATGCTCATGATGTGATCTCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC229892 representing NM_001174151
  Red=Cloning site Green=Tags(s)
  
  MEETKEAMSEMLRHPRISGKPILVLANKQDKEGALGEADVIECLSLEKLVNEHKCLCQIEPCSAISGYGK
  KIDKSIKKGLYWLLHVIARDFDALNERIQKETTEQRALEEQEKQERAERVRKLREERKQNEQEQAELDGT
  SGLAELDPEPTNPFQPIASVIIENEGKLEREKKNQKMEKDSDGCHLKHKMEHEQIETQGQVNHNGQKNNE
  FGLVENYKEALTQQLKNEDETDRPSLESANGKKKTKKLRMKRNHRVEPLNIDDCAPESPTPPPPPPPVGW
  GTPKVTRLPKLEPLGETHHNDFYRKPLPPLAVPQRPNSDAHDVIS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001174151
• ORF Size: 975 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001174151.1, NP_001167622.1
• RefSeq ORF: 978 bp
• Locus ID: 200894
• UniProt ID: Q3SXY8
• Cytogenetics: 3q11.1-q11.2
• MW: 37.5 kDa
• Gene Summary: This gene encodes a member of the ADP-ribosylation factor-like family. The encoded protein is a small GTPase that contains both N-terminal and C-terminal guanine nucleotide-binding motifs. This protein is localized in the cilia and plays a role in cilia formation and in maintenance of cilia. Mutations in this gene are the cause of Joubert syndrome 8. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]