TMEM185A (NM_001174092) Human Tagged ORF Clone

CAT#: RC229841

TMEM185A (Myc-DDK-tagged)-Human transmembrane protein 185A (TMEM185A), transcript variant 2

Product Images

Circular map for RC229841

• 

  Western blot validation of overexpression lysate (Cat# LY432841) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC229841 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TMEM185A
• Synonyms: CXorf13; ee3; FAM11A; FRAXF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC229841 representing NM_001174092
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCTGAGGGGCCTCTTCCAGGACTTCAACCCGAGAGCAGAAGGAGAAACGTGTGTGGAGTTTAAAG
  CCATGTTGATTGCAGTGGGCATCCACTTGCTCTTGTTGATGTTTGAAGTTCTGGTCTGTGACAGAATCGA
  GAGAGGAAGCCATTTCTGGCTCCTGGTCTTCATGCCGCTGTTCTTTGTTTCCCCGGTGTCTGTTGCAGCT
  TGCGTTTGGGGCTTTCGACATGACAGGTCACTAGAGTTAGAAATCCTGTGTTCTGTCAACATTCTCCAGT
  TTATATTCATTGCCTTAAGACTGGACAAGATCATCCACTGGCCCTGGCTTGTTGTGTGTGTCCCGCTGTG
  GATTCTCATGTCCTTTCTGTGCCTGGTGGTCCTCTACTACATTGTGTGGTCCGTCTTGTTCTTGCGCTCT
  ATGGATGTGATTGCAGAGCAGCGCAGGACACACATAACCATGGCCCTGAGCTGGATGACCATCGTCGTGC
  CCCTTCTTACATTTGAGATTCTGCTGGTTCACAAACTGGATGGCCACAACGCCTTCTCCTGCATCCCGAT
  CTTTGTCCCCCTTTGGCTCTCGTTGATCACGCTGATGGCAACCACATTTGGACAGAAGGGAGGAAACCAC
  TGGTGGTTTGGTATCCGCAAAGATTTCTGTCAGTTTCTGCTTGAAATCTTCCCATTTCTACGAGAATATG
  GAAACATTTCCTATGATCTCCATCACGAAGATAATGAAGAAACCGAAGAGACCCCAGTTCCGGAGCCCCC
  TAAAATCGCACCCATGTTTCGAAAGAAGGCCAGGGTGGTCATTACCCAGAGCCCTGGGAAGTATGTGCTC
  CCACCTCCCAAATTAAATATCGAAATGCCAGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC229841 representing NM_001174092
  Red=Cloning site Green=Tags(s)
  
  MNLRGLFQDFNPRAEGETCVEFKAMLIAVGIHLLLLMFEVLVCDRIERGSHFWLLVFMPLFFVSPVSVAA
  CVWGFRHDRSLELEILCSVNILQFIFIALRLDKIIHWPWLVVCVPLWILMSFLCLVVLYYIVWSVLFLRS
  MDVIAEQRRTHITMALSWMTIVVPLLTFEILLVHKLDGHNAFSCIPIFVPLWLSLITLMATTFGQKGGNH
  WWFGIRKDFCQFLLEIFPFLREYGNISYDLHHEDNEETEETPVPEPPKIAPMFRKKARVVITQSPGKYVL
  PPPKLNIEMPD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001174092
• ORF Size: 873 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001174092.1, NP_001167563.1
• RefSeq ORF: 876 bp
• Locus ID: 84548
• UniProt ID: Q8NFB2
• Cytogenetics: Xq28
• Protein Families: Transmembrane
• MW: 34.4 kDa
• Gene Summary: The protein encoded by this gene is predicted to be a transmembrane protein. This gene is best known for localizing to the CpG island of the fragile site FRAXF. The 5' untranslated region of this gene contains a CGG trinucleotide repeat sequence that normally consists of 7-40 tandem CGG repeats but which can expand to greater than 300 repeats. Methylation of the CpG island leads to transcriptional silencing of this gene, but neither the silencing nor an expanded repeat region appear to manifest itself in a clear phenotypic manner. Alternative splicing results in multiple transcript variants. A pseudogene of this gene has been defined on the X chromosome. [provided by RefSeq, Aug 2013]