SCAPER (NM_020843) Human Tagged ORF Clone

CAT#: RC229439

SCAPER (Myc-DDK-tagged)-Human S-phase cyclin A-associated protein in the ER (SCAPER), transcript variant 1

Product Images

Circular map for RC229439

• 

  Western blot validation of overexpression lysate (Cat# LY432447) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC229439 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SCAPER
• Synonyms: IDDRP; MSTP063; Zfp291; ZNF291
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC229439 representing NM_020843
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGCTTCATTCCAGCGCTCCAATAGTCATGACAAAGTAAGGAGAATAGTTGCAGAGGAGGGTCGTA
  CAGCAAGAAACCTAATAGCTTGGAGTGTTCCACTAGAAAGCAAAGATGATGATGGAAAACCTAAATGTCA
  AACTGGTGGAAAATCTAAGAGGACCATTCAAGGCACTCATAAAACTACTAAACAGAGTACTGCAGTGGAC
  TGTAAAATAACATCGTCTACGACTGGAGATAAACACTTTGATAAAAGTCCCACTAAAACAAGGCACCCTC
  GGAAAATTGATCTAAGAGCTCGATACTGGGCATTTCTTTTTGATAATCTTCGCCGAGCAGTAGATGAAAT
  CTATGTAACTTGCGAATCAGATCAGAGTGTGGTCGAATGTAAGGAGGTGCTAATGATGCTGGATAACTAT
  GTAAGAGATTTCAAAGCATTGATTGACTGGATTCAGCTTCAGGAAAAGCTAGAGAAGACAGATGCTCAAA
  GCAGACCAACATCATTGGCATGGGAAGTAAAGAAGATGTCTCCGGGACGCCATGTGATTCCAAGTCCATC
  AACAGATAGAATAAATGTAACATCAAATGCTCGACGAAGCTTAAATTTTGGAGGTTCAACTGGCACAGTG
  CCAGCTCCTCGTCTGGCTCCCACAGGTGTCAGTTGGGCTGACAAGGTAAAGGCTCATCATACAGGCTCTA
  CTGCTTCTTCAGAAATAACACCCGCCCAGTCTTGCCCACCAATGACAGTGCAGAAGGCCTCACGCAAAAA
  TGAACGGAAAGATGCTGAAGGATGGGAGACCGTTCAGAGAGGAAGGCCTATTCGTTCTCGATCAACAGCA
  GTGATGCCAAAAGTTTCATTGGCAACAGAAGCCACAAGATCAAAGGATGACAGTGATAAAGAAAATGTAT
  GTCTTTTACCTGATGAAAGCATACAGAAAGGTCAATTTGTTGGAGATGGAACTTCTAATACTATAGAATC
  TCATCCCAAAGACTCATTACACTCTTGTGACCATCCTCTTGCCGAAAAAACCCAGTTCACAGTGAGTACA
  TTGGATGATGTGAAGAATTCTGGCAGTATTCGAGACAATTATGTTCGAACTTCTGAAATATCTGCTGTCC
  ACATTGATACAGAGTGTGTTTCAGTTATGCTGCAAGCTGGTACACCTCCTTTACAAGTAAATGAAGAAAA
  ATTTCCAGCAGAGAAAGCAAGGATAGAAAATGAAATGGACCCTTCAGATATTTCAAATTCCATGGCAGAA
  GTCCTTGCTAAAAAAGAAGAGCTAGCAGATCGTCTAGAAAAGGCCAATGAAGAAGCCATTGCTAGTGCTA
  TTGCTGAAGAAGAACAGTTAACTAGAGAAATTGAAGCTGAAGAAAACAATGATATTAACATTGAAACTGA
  CAACGACAGTGATTTTTCTGCCAGCATGGGCAGTGGGAGTGTTTCTTTCTGTGGTATGTCCATGGACTGG
  AACGATGTCCTTGCAGATTATGAAGCTCGTGAGTCTTGGCGCCAAAATACATCCTGGGGGGACATTGTAG
  AAGAAGAACCTGCTAGACCTCCAGGGCATGGAATTCACATGCATGAAAAACTTTCTTCACCCTCTCGTAA
  AAGAACAATTGCAGAATCTAAGAAGAAACATGAAGAAAAACAAATGAAAGCACAGCAGCTAAGGGAAAAG
  TTACGCGAAGAGAAAACATTGAAGCTTCAGAAATTGTTAGAAAGGGAGAAGGATGTCCGGAAGTGGAAGG
  AAGAATTGCTAGATCAACGACGCAGGATGATGGAAGAAAAATTACTTCATGCTGAGTTTAAGCGAGAAGT
  GCAGTTACAAGCAATTGTGAAAAAAGCACAAGAAGAAGAAGCTAAGGTAAATGAAATTGCCTTTATAAAT
  ACCCTTGAAGCCCAGAATAAACGTCATGATGTTTTATCAAAATTGAAGGAATATGAACAGAGGCTTAATG
  AGCTACAGGAAGAGCGTCAGAGAAGACAGGAAGAAAAGCAAGCACGTGATGAAGCTGTGCAGGAACGCAA
  GAGAGCTCTAGAGGCAGAGCGGCAGGCCCGTGTAGAAGAATTGTTAATGAAGAGGAAAGAACAAGAAGCC
  CGAATTGAACAACAGAGGCAAGAAAAGGAAAAAGCCCGTGAGGATGCAGCCCGGGAAAGAGCTAGAGACA
  GGGAAGAACGATTGGCAGCACTCACAGCTGCTCAACAAGAAGCTATGGAAGAGTTACAGAAAAAAATTCA
  GCTCAAGCATGATGAAAGTATTCGAAGGCACATGGAACAGATTGAACAAAGAAAAGAAAAAGCTGCTGAG
  CTAAGCAGTGGGCGACATGCAAATACTGATTATGCCCCCAAACTGACCCCTTATGAAAGAAAGAAGCAGT
  GTTCTCTCTGCAATGTCCTGATCTCTTCAGAGGTATATCTTTTTAGCCATGTTAAAGGGAGAAAACACCA
  GCAAGCCGTGAGAGAGAATACCAGCATCCAGGGGCGTGAACTGTCAGATGAAGAAGTGGAGCATCTTTCC
  TTGAAGAAGTACATTATTGACATTGTGGTTGAAAGTACAGCTCCAGCAGAAGCTTTGAAAGATGGAGAAG
  AGCGGCAAAAAAATAAAAAAAAAGCCAAAAAGATAAAAGCCCGGATGAACTTCAGGGCTAAGGAATATGA
  GAGTTTAATGGAAACCAAAAATTCTGGCTCTGATTCACCTTATAAAGCAAAGCTTCAGCGATTAGCCAAA
  GATCTTCTAAAACAAGTACAAGTTCAAGACAGTGGCTCATGGGCAAACAATAAAGTGTCTGCTTTGGATC
  GGACCCTAGGAGAGATCACTAGAATACTGGAAAAAGAGAATGTGGCAGATCAGATTGCATTTCAAGCTGC
  TGGTGGATTAACAGCCCTTGAACACATCCTTCAAGCAGTAGTCCCAGCCACAAATGTGAACACAGTTTTA
  AGAATTCCTCCTAAGTCTCTCTGCAATGCAATCAATGTTTACAACCTCACCTGCAATAACTGTTCAGAAA
  ACTGCAGTGATGTTCTGTTTAGTAACAAGATTACCTTCTTAATGGACCTCCTGATACACCAGTTGACGGT
  TTATGTTCCAGATGAAAATAATACTATTTTGGGGAGAAATACAAATAAACAAGTTTTTGAAGGCTTGACA
  ACTGGACTTCTCAAAGTCAGTGCTGTGGTTTTGGGCTGCCTGATTGCCAATCGACCAGATGGAAACTGCC
  AGCCAGCTACCCCAAAAATACCAACACAGGAAATGAAAAACAAACCCTCACAAGGTGATCCTTTTAACAA
  TCGAGTTCAGGACCTTATCAGCTACGTGGTGAACATGGGTCTGATTGACAAACTGTGTGCCTGCTTCCTC
  TCGGTGCAAGGCCCAGTGGATGAGAATCCCAAGATGGCCATATTTCTGCAGCATGCCGCAGGACTCTTAC
  ATGCAATGTGTACACTGTGCTTTGCTGTCACTGGAAGGTCATACAGCATATTTGACAATAATCGCCAGGA
  TCCCACAGGGCTGACAGCTGCTCTTCAGGCAACCGACCTGGCTGGAGTTCTTCATATGCTCTACTGTGTC
  CTCTTCCATGGCACCATCTTGGACCCCAGCACTGCCAGTCCCAAGGAGAATTACACTCAAAATACCATCC
  AAGTGGCCATTCAGAGTTTACGTTTCTTCAACAGCTTTGCAGCTCTTCATCTGCCTGCTTTTCAGTCTAT
  TGTAGGGGCAGAGGGCTTGTCCCTTGCATTCCGGCACATGGCCAGCTCCCTGCTGGGCCACTGCAGCCAA
  GTCTCCTGTGAAAGCCTCCTTCATGAGGTCATCGTCTGTGTGGGCTACTTCACTGTCAACCACCCAGATA
  ACCAGGTGATCGTGCAGTCCGGCCGCCACCCCACAGTGCTGCAGAAGCTCTGCCAGTTGCCCTTCCAGTA
  TTTCAGTGACCCACGGCTGATCAAAGTACTGTTCCCTTCACTTATCGCTGCTTGTTACAACAACCATCAG
  AACAAGATCATTCTGGAGCAAGAGATGAGCTGTGTTTTACTGGCCACTTTCATTCAGGATTTGGCACAGA
  CTCCAGGTCAAGCGGAAAACCAGCCTTACCAACCCAAAGGGAAATGCCTTGGTTCCCAAGACTATCTTGA
  GCTGGCTAACAGATTTCCTCAGCAGGCCTGGGAAGAAGCTCGACAGTTTTTCTTGAAAAAAGAGAAAAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC229439 representing NM_020843
  Red=Cloning site Green=Tags(s)
  
  MMASFQRSNSHDKVRRIVAEEGRTARNLIAWSVPLESKDDDGKPKCQTGGKSKRTIQGTHKTTKQSTAVD
  CKITSSTTGDKHFDKSPTKTRHPRKIDLRARYWAFLFDNLRRAVDEIYVTCESDQSVVECKEVLMMLDNY
  VRDFKALIDWIQLQEKLEKTDAQSRPTSLAWEVKKMSPGRHVIPSPSTDRINVTSNARRSLNFGGSTGTV
  PAPRLAPTGVSWADKVKAHHTGSTASSEITPAQSCPPMTVQKASRKNERKDAEGWETVQRGRPIRSRSTA
  VMPKVSLATEATRSKDDSDKENVCLLPDESIQKGQFVGDGTSNTIESHPKDSLHSCDHPLAEKTQFTVST
  LDDVKNSGSIRDNYVRTSEISAVHIDTECVSVMLQAGTPPLQVNEEKFPAEKARIENEMDPSDISNSMAE
  VLAKKEELADRLEKANEEAIASAIAEEEQLTREIEAEENNDINIETDNDSDFSASMGSGSVSFCGMSMDW
  NDVLADYEARESWRQNTSWGDIVEEEPARPPGHGIHMHEKLSSPSRKRTIAESKKKHEEKQMKAQQLREK
  LREEKTLKLQKLLEREKDVRKWKEELLDQRRRMMEEKLLHAEFKREVQLQAIVKKAQEEEAKVNEIAFIN
  TLEAQNKRHDVLSKLKEYEQRLNELQEERQRRQEEKQARDEAVQERKRALEAERQARVEELLMKRKEQEA
  RIEQQRQEKEKAREDAARERARDREERLAALTAAQQEAMEELQKKIQLKHDESIRRHMEQIEQRKEKAAE
  LSSGRHANTDYAPKLTPYERKKQCSLCNVLISSEVYLFSHVKGRKHQQAVRENTSIQGRELSDEEVEHLS
  LKKYIIDIVVESTAPAEALKDGEERQKNKKKAKKIKARMNFRAKEYESLMETKNSGSDSPYKAKLQRLAK
  DLLKQVQVQDSGSWANNKVSALDRTLGEITRILEKENVADQIAFQAAGGLTALEHILQAVVPATNVNTVL
  RIPPKSLCNAINVYNLTCNNCSENCSDVLFSNKITFLMDLLIHQLTVYVPDENNTILGRNTNKQVFEGLT
  TGLLKVSAVVLGCLIANRPDGNCQPATPKIPTQEMKNKPSQGDPFNNRVQDLISYVVNMGLIDKLCACFL
  SVQGPVDENPKMAIFLQHAAGLLHAMCTLCFAVTGRSYSIFDNNRQDPTGLTAALQATDLAGVLHMLYCV
  LFHGTILDPSTASPKENYTQNTIQVAIQSLRFFNSFAALHLPAFQSIVGAEGLSLAFRHMASSLLGHCSQ
  VSCESLLHEVIVCVGYFTVNHPDNQVIVQSGRHPTVLQKLCQLPFQYFSDPRLIKVLFPSLIAACYNNHQ
  NKIILEQEMSCVLLATFIQDLAQTPGQAENQPYQPKGKCLGSQDYLELANRFPQQAWEEARQFFLKKEKK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020843
• ORF Size: 4200 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020843.4
• RefSeq ORF: 4203 bp
• Locus ID: 49855
• UniProt ID: Q9BY12
• Cytogenetics: 15q24.3
• Domains: ZnF_U1
• MW: 158.1 kDa
• Gene Summary: CCNA2/CDK2 regulatory protein that transiently maintains CCNA2 in the cytoplasm.[UniProtKB/Swiss-Prot Function]