NHSL2 (NM_001013627) Human Tagged ORF Clone

CAT#: RC229419

NHSL2 (Myc-DDK-tagged)-Human NHS-like 2 (NHSL2)

Product Images

Circular map for RC229419

• 

  Western blot validation of overexpression lysate (Cat# LY432427) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC229419 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NHSL2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC229419 representing NM_001013627
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGTTCTACAGGCGCACGGTGGTACCCCAGCGCCTGTGCCCGCGCAACCCGCCGCAGCAGCTGGCGG
  AGCTCCGCGACGTGAGCCACCTGGCAGCGCTCAGCCTGCTCCGGCAGCTCGCCGACCTCTGTGGCCACTC
  GTTGGCTCTGCTCGAGGACCTCGAGGGGCACCTGCTGGCCCTGGGGCGCCGCACAGACAGCCTGTACCGG
  CGCACCGTGCGCCTCCGCCGCCGCCTTCCCTGCCGCCTGCTTGGCCCGGAGGAGGACGAGGAAGAGCTAG
  CTGCAGCTAACTCGGGTCGGGAAAATGCGACAGCGACTGCCCACTCGAGGTCGTCATGGCGACAGCCAGT
  GAACGTGTTCCTCTCCTCGGGCAGGCCCCCGAGTGTAGAGGAGCTGCTTCGGGAGGCGCAGCTCAATCTC
  CAGAGCCTGTTGCAAGAAGAATATGAGGAACAGTACTCGGAGGCCAGACTTGTGGGGCAGACCTTCCGCT
  CTTCTGATGAGGCCACTAAGCCCACCCCCAACCCAAGGCCCCAGTCTGCCAGGCGTCTGGAGTTTATATT
  GATGCCTACAAAACGGCAGCTGAGCGAGGATGAGACTACCACCCAGGGTGTGAGGGCCCCCGAGGCCTCC
  CTGAGCCTGTCTACCACAGCCGACAAGCAAACTGCCTGGAATAGCCTTTTCCCTCTGCCCATCCTAGAGG
  AGAAGCGGTGGCCTCAGCTTTGCTCCACGCAGTCTGACATTGTGCCCATCAACATCTCTGGGCAGCAGTT
  TGATAAACATGCAAGTTTGCGACACTCGTTGTTTAACACAGAGACAGCCGTGAACCCCAAGTCCACCCTG
  AGGCGGAGGCGGACCATTATTGGATTCTCTAACTTTTCCCAGCGAGACCAAGGTCACAGCAACAGCCCAG
  CAGGCAGTGTGGCCCACTCTACCACCTCCGACATCAGGCCCAGTCACTCAGTTCCAGAAGGGGTTCATGG
  AAGAGTTGCAGTTGGTCAGGATGCTCGGTTCCCAAGTCTCACCTCGCCAGTACTGAGAACTCCTTCCAGT
  GAGCCGGACGAACCTCACCAGGCACGGAGTGGCCCAAACCCTCCTGGCATGGAGAGCATGGGAATGGTGT
  ACAGTGTCCCCAGTTCTTGCAATGGACCTACAGAATCAACCTTCTCCACTTCCTGGAAGGGAGATGCTTT
  TACCTACATGACTCCAAGTGCCACCAGCCAGAGCAATCAAGTCAATGAAAATGGGAAAAATCCTTCCTGT
  GGGAATTCTTGGGTCTCTCTAAACAAAGTCCCACCTCTGGTTCCTAAGGAGGCTGCTACCCTCCTTGTCG
  CTCGTGATAACCCAGCAGGATGCAGTGGGTCAGCTGGCTACCCTGAGCGCCTTATTCAGCAAAGGCACAT
  GCCCGAAAGACCCTCCAAGATTGGCCTTCTGACCAGTGGGACCTCGAGGCTGGAGACAGGCCCCGGTGGG
  GCCAGCAGATTCCGGGAGCGGTCACTGTCTGTGCCCACAGACTCAGGCACCACAGATGTGGACTATGATG
  AGGAGCAGAAGGCCAATGAGGCCTGTGCCCTGCCTTTTGCCAGTACGAGCTCTGAGGGCAGTAACAGTGC
  TGACAACATTGCCTCCCTTAGTGCCCAGCAAGAGGCCCAGCACAGAAGGCAGAGGTCCAAGAGTATCTCA
  CTTAGGAAGGCCAAAAAGAAGCCTTCCCCACCCACACGCAGTGTCTCACTGGTCAAAGATGAGCCAGGCC
  TCTTGCCTGAAGGTGGGTCAGCACTACCCAAGGACCAGAGGCCCAAGAGCCTTTGCCTCTCCTTGGAACA
  TCAAGGACATCACTCGTCCCACCCAGATGCTCAGGGTCACCCAGCTATTCCAAACCACAAAGATCCAGAA
  AGTACACAATTCTCCCACCACTGGTATCTTACTGACTGGAAGTCTGGTGACACCTACCAATCCCTGTCCA
  GCTCCAGCACTGCCACTGGCACCACAGTCATTGAGTGCACCCAAGTTCAGGGCAGCTCAGAGTCTCTTGC
  CTCACCTTCCACCTCCAGAGCCACTACACCTTCCCAACTCTCCATTGAAGTGGAGGCCAGGGAGATATCA
  TCCCCGGGAAGGCCCCCTGGACTGATGTCACCCTCCAGTGGCTACTCCAGCCAGTCGGAAACACCAACAC
  CCACTGTTTCCATGTCCCTGACCCTGGGCCACTTACCCCCTCCAAGCAGCAGTGTCCGGGTACGTCCAGT
  GGTACCTGAGAGGAAGTCATCACTACCCCCGACGTCACCAATGGAGAAATTTCCCAAGTCACGGCTATCA
  TTTGACCTACCACTGACCTCTTCACCCAACCTGGATCTGTCTGGGATGAGTATCTCCATCCGAAGCAAAA
  CTAAGGTGAGTCGGCACCACTCAGAGACAAATTTTGGCGTCAAGCTGGCCCAGAAAACTAATCCCAACCA
  GCCAATCATGCCTATGGTTACTCAGTCCGACCTACGTTCTGTTCGCCTGAGGTCGGTCAGCAAGTCTGAG
  CCGGAAGATGATATTGAGAGCCCTGAGTATGCCGAGGAACCCAGAGCAGAAGAAGTCTTCACCTTGCCAG
  AGAGAAAGACAAAACCTCCCGTAGCTGAGAAGCCTCCGGTGGCCCGGAGGCCTCCAAGCTTGGTCCACAA
  GCCACCATCTGTTCCTGAGGAGTATGCACTAACTTCACCAACCTTGGCTATGCCCCCCAGGAGCTCAATT
  CAACATGCGAGACCACTCCCTCAAGACAGCTACACGGTAGTGCGGAAACCAAAGCCCTCCAGCTTCCCAG
  ATGGCAGAAGCCCAGGGGAGTCAACAGCACCCTCATCTCTTGTTTTCACGCCTTTTGCCAGTTCCTCTGA
  TGCTTTCTTCTCAGGAACACAGCAGCCTCCCCAGGGAAGTGTAGAGGACGAGGGCCCCAAGGTGAGGGTT
  CTGCCTGAAAGAATTAGCCTCCAGAGCCAGGAAGAAGCTGAGAAAAAGAAAGGCAAGATTCCACCTCCCG
  TACCAAAAAAACCCAGCGTGCTGTACCTGCCTCTCACTTCTCCCACAGCTCAAATGGAGGCCTATGTGGC
  AGAACCAAGGCTGCCTCTCAGCCCCATCATCACCCTGGAGGAAGACACCAAGTGTCCCGCCACCGGCGAT
  GACCTGCAATCACTTGGTCAAAGGGTGACTTCAACTCCTCAGGCTGACAGTGAAAGGGAGGCAAGCCCTC
  TGGGGAGTTCTGTGGAACCAGGCACCGAAGAAAAAAGTTTAATCAGTGATAAAACAGCTGAATGGATTGC
  AGAGGATGATGATGACGTGTTTGTGGCTTCACGCACAACTGAAGATTTATTTACTGTGATACACAGGTCC
  AAAAGGAAGCTGCTCGGCTGGAAGGAACCTGGTGAGGCCTTTGTGGGTGGCAGAACGAGTTCCCACTCAC
  CAATAAAGAACACAGCTGAGTCTCCAATCAGTGAGTCTACCGCCACTGCAGGGTCAGGCAGCAGTGCCAA
  CCTAGATGCTGGCAGAAATGACGATTTCAAGGCCTTGCTACAGAAGAAGGGAAGTAAGGCAACTCCAAGG
  TCTCGTCCCTCAGCAGCTGAACTTCTGAAGACCACTAACCCACTGGCTCGGAGAATTATTGCACAATTTT
  CAAAAGACTATGAAACCACCGATAACCCCAGTACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC229419 representing NM_001013627
  Red=Cloning site Green=Tags(s)
  
  MPFYRRTVVPQRLCPRNPPQQLAELRDVSHLAALSLLRQLADLCGHSLALLEDLEGHLLALGRRTDSLYR
  RTVRLRRRLPCRLLGPEEDEEELAAANSGRENATATAHSRSSWRQPVNVFLSSGRPPSVEELLREAQLNL
  QSLLQEEYEEQYSEARLVGQTFRSSDEATKPTPNPRPQSARRLEFILMPTKRQLSEDETTTQGVRAPEAS
  LSLSTTADKQTAWNSLFPLPILEEKRWPQLCSTQSDIVPINISGQQFDKHASLRHSLFNTETAVNPKSTL
  RRRRTIIGFSNFSQRDQGHSNSPAGSVAHSTTSDIRPSHSVPEGVHGRVAVGQDARFPSLTSPVLRTPSS
  EPDEPHQARSGPNPPGMESMGMVYSVPSSCNGPTESTFSTSWKGDAFTYMTPSATSQSNQVNENGKNPSC
  GNSWVSLNKVPPLVPKEAATLLVARDNPAGCSGSAGYPERLIQQRHMPERPSKIGLLTSGTSRLETGPGG
  ASRFRERSLSVPTDSGTTDVDYDEEQKANEACALPFASTSSEGSNSADNIASLSAQQEAQHRRQRSKSIS
  LRKAKKKPSPPTRSVSLVKDEPGLLPEGGSALPKDQRPKSLCLSLEHQGHHSSHPDAQGHPAIPNHKDPE
  STQFSHHWYLTDWKSGDTYQSLSSSSTATGTTVIECTQVQGSSESLASPSTSRATTPSQLSIEVEAREIS
  SPGRPPGLMSPSSGYSSQSETPTPTVSMSLTLGHLPPPSSSVRVRPVVPERKSSLPPTSPMEKFPKSRLS
  FDLPLTSSPNLDLSGMSISIRSKTKVSRHHSETNFGVKLAQKTNPNQPIMPMVTQSDLRSVRLRSVSKSE
  PEDDIESPEYAEEPRAEEVFTLPERKTKPPVAEKPPVARRPPSLVHKPPSVPEEYALTSPTLAMPPRSSI
  QHARPLPQDSYTVVRKPKPSSFPDGRSPGESTAPSSLVFTPFASSSDAFFSGTQQPPQGSVEDEGPKVRV
  LPERISLQSQEEAEKKKGKIPPPVPKKPSVLYLPLTSPTAQMEAYVAEPRLPLSPIITLEEDTKCPATGD
  DLQSLGQRVTSTPQADSEREASPLGSSVEPGTEEKSLISDKTAEWIAEDDDDVFVASRTTEDLFTVIHRS
  KRKLLGWKEPGEAFVGGRTSSHSPIKNTAESPISESTATAGSGSSANLDAGRNDDFKALLQKKGSKATPR
  SRPSAAELLKTTNPLARRIIAQFSKDYETTDNPST
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001013627
• ORF Size: 3675 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001013627.2, NP_001013649.2
• RefSeq ORF: 3678 bp
• Locus ID: 340527
• UniProt ID: Q5HYW2
• Cytogenetics: Xq13.1
• MW: 133.1 kDa