CMIP (NM_198390) Human Tagged ORF Clone

CAT#: RC229369

CMIP (Myc-DDK-tagged)-Human c-Maf-inducing protein (CMIP), transcript variant C-mip

Product Images

Circular map for RC229369

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  Western blot validation of overexpression lysate (Cat# LY432383) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC229369 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CMIP protein (Cat# TP329369). The protein was produced from HEK293T cells transfected with CMIP cDNA clone (Cat# RC229369) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CMIP
• Synonyms: TCMIP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC229369 representing NM_198390
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGTGACCAGCAGCTCGGGCGGCGGCGGCGACCCCCGGCAGATCGAGGAGACCAAGCCGCTGCTGG
  GGGGCGACGTGTCGGCCCCCGAAGGCACGAAGATGGGCGCCGTGCCCTGCCGCCGGGCTCTTCTGCTTTG
  CAACGGGATGAGGTACAAACTGCTGCAGGAGGGCGACATTCAGGTCTGTGTCATCCGGCACCCGCGGACC
  TTTCTCAGCAAGATCCTCACCTCGAAATTCCTGAGGCGCTGGGAGCCGCACCACCTAACGCTGGCCGACA
  ACAGCCTGGCGTCCGCCACGCCAACTGGGTACATGGAAAACTCAGTCTCCTACAGCGCAATTGAAGACGT
  TCAGCTGCTGTCCTGGGAGAATGCCCCGAAGTACTGTTTACAGCTCACGATTCCTGGGGGAACTGTCTTA
  CTGCAGGCTGCCAATAGCTACCTGCGAGACCAGTGGTTCCATTCTCTGCAATGGAAGAAAAAGATTTACA
  AATATAAGAAAGTGCTGAGTAACCCAAGCCGCTGGGAAGTTGTCTTGAAAGAGATCCGGACCCTGGTGGA
  CATGGCCCTGACATCCCCCCTGCAGGATGACTCCATCAACCAGGCCCCACTGGAAATCGTCTCGAAACTG
  CTCTCAGAGAACACAAACTTGACCACCCAGGAGCATGAAAACATCATTGTGGCAATCGCTCCTTTGCTGG
  AAAACAACCACCCACCACCAGATCTCTGTGAATTCTTTTGCAAGCACTGCAGAGAGCGGCCCCGGTCCAT
  GGTGGTCATCGAGGTGTTCACCCCCGTGGTGCAGCGAATCCTCAAGCATAACATGGACTTTGGGAAGTGC
  CCGCGACTGAGGCTGTTTACTCAGGAGTACATCCTTGCCTTGAACGAGCTCAACGCGGGGATGGAAGTGG
  TGAAGAAGTTCATTCAGAGCATGCACGGCCCCACAGGGCACTGCCCCCACCCCCGGGTCCTGCCCAACCT
  GGTGGCCGTGTGCCTGGCTGCCATCTACTCCTGCTATGAAGAGTTCATCAACAGCCGCGACAATTCCCCA
  AGCCTGAAGGAAATCCGGAACGGCTGCCAGCAGCCGTGCGACCGGAAGCCCACTTTACCTCTGCGCCTTC
  TGCACCCCAGCCCGGACCTGGTGTCTCAGGAAGCCACGCTGTCTGAGGCCCGGCTCAAGTCGGTGGTCGT
  GGCCTCCAGTGAGATCCACGTGGAGGTGGAACGCACCAGCACTGCCAAGCCGGCGCTGACGGCCAGCGCA
  GGCAACGACAGCGAGCCCAACCTCATCGACTGCCTCATGGTCAGCCCCGCCTGCAGCACCATGAGCATCG
  AGCTGGGCCCCCAGGCCGACCGCACGCTCGGCTGCTACGTGGAAATCCTCAAGCTGCTGTCAGACTATGA
  TGACTGGAGACCGTCTCTGGCCAGTTTGCTTCAACCCATTCCATTCCCCAAAGAAGCTCTCGCACATGAG
  AAGTTCACCAAGGAACTGAAGTACGTGATTCAGAGGTTCGCCGAAGACCCCAGGCAAGAGGTCCACTCAT
  GCCTGCTGAGCGTGCGGGCCGGCAAAGATGGCTGGTTCCAGCTCTACAGCCCCGGAGGGGTGGCCTGCGA
  CGATGACGGGGAGCTGTTCGCCAGCATGGTGCACATCCTCATGGGCTCCTGTTACAAGACCAAAAAATTC
  CTGCTCTCCCTGGCAGAAAACAAGCTGGGTCCCTGCATGCTCCTGGCACTGAGGGGGAACCAGACCATGG
  TGGAGATCCTGTGCTTGATGCTGGAATACAACATCATCGACAACAACGACACCCAACTGCAGATCATCTC
  AACCCTGGAGAGCACAGACGTGGGGAAGCGCATGTACGAGCAGCTGTGTGACCGGCAGCGGGAGCTGAAG
  GAGCTGCAAAGGAAAGGCGGGCCCACCAGGCTAACACTGCCCTCCAAGTCCACAGACGCTGACTTGGCTC
  GTTTGCTGAGCTCCGGCTCCTTCGGAAACCTGGAGAACCTCAGTTTGGCCTTCACCAATGTAACCAGTGC
  CTGCGCCGAGCACCTCATCAAACTGCCTTCGCTCAAGCAGCTGAACCTGTGGTCCACTCAGTTTGGAGAC
  GCTGGCCTTCGGCTCCTGTCGGAACACCTCACCATGCTCCAGGTGCTGAACCTGTGCGAGACCCCGGTCA
  CAGACGCTGGCCTGCTGGCCCTGAGCTCCATGAAGAGTCTCTGCAGTTTAAACATGAACAGCACCAAGCT
  CTCAGCTGACACCTACGAAGATCTGAAGGCCAAGCTTCCCAATTTGAAGGAAGTGGACGTCCGCTACACC
  GAAGCCTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC229369 representing NM_198390
  Red=Cloning site Green=Tags(s)
  
  MDVTSSSGGGGDPRQIEETKPLLGGDVSAPEGTKMGAVPCRRALLLCNGMRYKLLQEGDIQVCVIRHPRT
  FLSKILTSKFLRRWEPHHLTLADNSLASATPTGYMENSVSYSAIEDVQLLSWENAPKYCLQLTIPGGTVL
  LQAANSYLRDQWFHSLQWKKKIYKYKKVLSNPSRWEVVLKEIRTLVDMALTSPLQDDSINQAPLEIVSKL
  LSENTNLTTQEHENIIVAIAPLLENNHPPPDLCEFFCKHCRERPRSMVVIEVFTPVVQRILKHNMDFGKC
  PRLRLFTQEYILALNELNAGMEVVKKFIQSMHGPTGHCPHPRVLPNLVAVCLAAIYSCYEEFINSRDNSP
  SLKEIRNGCQQPCDRKPTLPLRLLHPSPDLVSQEATLSEARLKSVVVASSEIHVEVERTSTAKPALTASA
  GNDSEPNLIDCLMVSPACSTMSIELGPQADRTLGCYVEILKLLSDYDDWRPSLASLLQPIPFPKEALAHE
  KFTKELKYVIQRFAEDPRQEVHSCLLSVRAGKDGWFQLYSPGGVACDDDGELFASMVHILMGSCYKTKKF
  LLSLAENKLGPCMLLALRGNQTMVEILCLMLEYNIIDNNDTQLQIISTLESTDVGKRMYEQLCDRQRELK
  ELQRKGGPTRLTLPSKSTDADLARLLSSGSFGNLENLSLAFTNVTSACAEHLIKLPSLKQLNLWSTQFGD
  AGLRLLSEHLTMLQVLNLCETPVTDAGLLALSSMKSLCSLNMNSTKLSADTYEDLKAKLPNLKEVDVRYT
  EAW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_198390
• ORF Size: 2319 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198390.2, NP_938204.2
• RefSeq ORF: 2322 bp
• Locus ID: 80790
• UniProt ID: Q8IY22
• Cytogenetics: 16q23.2-q23.3
• MW: 86.2 kDa
• Gene Summary: This gene encodes a c-Maf inducing protein that plays a role in T-cell signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011]