EGR4 (NM_001965) Human Tagged ORF Clone

CAT#: RC229296

EGR4 (Myc-DDK-tagged)-Human early growth response 4 (EGR4)

Product Images

Circular map for RC229296

• 

  Western blot validation of overexpression lysate (Cat# LY432313) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC229296 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EGR4
• Synonyms: AT133; NGFI-C; NGFIC; PAT133
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC229296 representing NM_001965
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGTGGCCCGGGGAGTCGGAAGCCCGGAGCCAGCGCCGCCGCAGCTATATAAGTGGGGGGGCTGTG
  GGCTGGGGGAGCCCGGCAGCGCTTTGGAGAGGCGAGGAGCCGCCGCCCGAGGCCGGTGCGGGCGAGCGAG
  GGCGCCGCGGCTCCCCGACTCCTTTCCCAGAGGTGAGTGCCCGAAGCCAGGAGCCCGGGCGCCTAGGTCT
  GTGCGCTGCGGGGAACCCCTACCGCCAGCCTCCCCGCCACCCGCGCGCCCCCAAGCCCAGCGGGCGAGGC
  CCCGGGCGCCCCACAGCCGGCGCCGCGCCATGCTCCACCTTAGCGAGTTTTCCGAACCCGACGCGCTCCT
  CGTCAAGTCCACTGAAGGCTGTTGCGCCGAACCCAGCGCTGAATTGCCCCGGCTGCCTGCCAGGGACGCT
  CCCGCGGCCACCGGCTACCCTGGAGCAGGCGACTTCTTGAGCTGGGCTTTGAACAGCTGCGGCGCAAGTG
  GGGACTTAGCCGACTCCTGCTTCCTGGAGGGGCCTGCGCCCACACCCCCTCCCGGCCTCAGCTACAGCGG
  TAGCTTCTTCATTCAGGCAGTGCCCGAACACCCGCACGACCCGGAGGCACTCTTCAACCTCATGTCGGGC
  ATCTTAGGCCTGGCACCCTTCCCCGGTCCAGAGGCAGCAGCGTCCAGATCCCCGCTGGATGCCCCTTTTC
  CTGCGGGGTCCGATGCCTTGCTGCCGGGTCCGCCGGACCTTTACTCCCCGGATCTGGGCGCTGCCCCTTT
  CCCAGAGGCGTTCTGGGAGGCCTCGCCTTGCGCGGGTGCCCCCTCGCAGTGCCTGTATGAGCCTCAGCTC
  TCCCCGCCCGACGTCAAGCCCGGCCTCCGGGCGCCTCCCGCCTCGCCAGCGCTGGACGCTGTCTCTGCCT
  TCAAGGGTCCCTACGCGCCCTGGGAGCTGCTTTCTGTGGGGGCCCCAGGGAACTGTGGGTCACAGGGAGA
  CTACCAGGCCGCCCCGGAGGCTCGTTTTCCCGTAATAGGGACCAAGATTGAGGACTTGCTGTCCATCAGC
  TGCCCTGCGGAACTGCCGGCCGTCCCAGCCAACAGACTCTATCCCAGCGGGGCCTATGACGCTTTCCCGC
  TGGCCCCGGGTGACTTAGGGGAGGGGGCTGAGGGCCTCCCTGGGCTCCTGACCCCTCCTAGTGGGGAGGG
  AGGGAGTAGCGGCGACGGCGGAGAGTTTCTGGCCAGTACGCAGCCTCAGCTTTCCCCGCTGGGCCTTCGC
  AGCGCCGCCGCGGCGGACTTCCCTAAACCTCTGGTGGCGGACATCCCTGGAAGCAGTGGCGTGGCTGCAC
  CACCCGTGCCGCCGCCGCCGCCCACCCCTTTCCCCCAGGCCAAGGCGCGACGCAAGGGGCGCCGCGGCGG
  CAAATGCAGCACGCGCTGCTTCTGCCCGCGGCCGCACGCCAAGGCCTTCGCTTGCCCGGTGGAGAGTTGT
  GTGCGGAGCTTTGCGCGCTCCGACGAGCTCAATCGCCACCTGCGCATCCACACGGGCCACAAACCCTTCC
  AGTGCCGCATCTGCCTCCGCAACTTCAGCCGCAGCGACCACCTCACCACGCACGTGCGCACCCACACCGG
  CGAGAAGCCTTTTGCTTGCGACGTGTGCGGCCGCCGCTTCGCGCGCAGCGATGAGAAGAAACGGCACAGC
  AAGGTGCACCTCAAGCAGAAGGCGCGCGCCGAGGAGCGGCTCAAGGGCCTCGGCTTTTACTCGCTGGGCC
  TCTCCTTCGCTTCTCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC229296 representing NM_001965
  Red=Cloning site Green=Tags(s)
  
  MAVARGVGSPEPAPPQLYKWGGCGLGEPGSALERRGAAARGRCGRARAPRLPDSFPRGECPKPGARAPRS
  VRCGEPLPPASPPPARPQAQRARPRAPHSRRRAMLHLSEFSEPDALLVKSTEGCCAEPSAELPRLPARDA
  PAATGYPGAGDFLSWALNSCGASGDLADSCFLEGPAPTPPPGLSYSGSFFIQAVPEHPHDPEALFNLMSG
  ILGLAPFPGPEAAASRSPLDAPFPAGSDALLPGPPDLYSPDLGAAPFPEAFWEASPCAGAPSQCLYEPQL
  SPPDVKPGLRAPPASPALDAVSAFKGPYAPWELLSVGAPGNCGSQGDYQAAPEARFPVIGTKIEDLLSIS
  CPAELPAVPANRLYPSGAYDAFPLAPGDLGEGAEGLPGLLTPPSGEGGSSGDGGEFLASTQPQLSPLGLR
  SAAAADFPKPLVADIPGSSGVAAPPVPPPPPTPFPQAKARRKGRRGGKCSTRCFCPRPHAKAFACPVESC
  VRSFARSDELNRHLRIHTGHKPFQCRICLRNFSRSDHLTTHVRTHTGEKPFACDVCGRRFARSDEKKRHS
  KVHLKQKARAEERLKGLGFYSLGLSFASL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001965
• ORF Size: 1767 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001965.3, NP_001956.3
• RefSeq ORF: 1461 bp
• Locus ID: 1961
• UniProt ID: Q05215
• Cytogenetics: 2p13.2
• MW: 61.4 kDa
• Gene Summary: Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-GCGGGGGCG-3' (GSG). Activates the transcription of target genes whose products are required for mitogenesis and differentiation (By similarity).[UniProtKB/Swiss-Prot Function]