ALDH4A1 (NM_001161504) Human Tagged ORF Clone

CAT#: RC228933

ALDH4A1 (Myc-DDK-tagged)-Human aldehyde dehydrogenase 4 family, member A1 (ALDH4A1), transcript variant 3

Product Images

Circular map for RC228933

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ALDH4A1
• Synonyms: ALDH4; P5CD; P5CDh
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC228933 representing NM_001161504
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGCCATCCCATGCGTGGTGGGGGATGAGGAGGTGTGGACGTCGGACGTGCAGTACCAAGTGTCGC
  CTTTTAACCATGGACATAAGGTGGCCAAGTTCTGTTATGCAGACAAGAGCCTGCTCAACAAAGCCATTGA
  GGCTGCCCTGGCTGCCCGGAAAGAGTGGGACCTGAAGCCTATTGCAGACCGGGCCCAGATCTTCCTGAAG
  GCGGCAGACATGCTGAGTGGGCCGCGCAGGGCTGAGATCCTCGCCAAGACCATGGTGGGACAGGGTAAGA
  CCGTGATCCAAGCGGAGATTGACGCTGCAGCGGAACTCATCGACTTCTTCCGGTTCAATGCCAAGTATGC
  GGTGGAGCTGGAGGGGCAGCAGCCCATCAGCGTGCCCCCGAGCACCAACAGCACGGTGTACCGGGGTCTG
  GAGGGCTTCGTGGCGGCCATCTCGCCCTTTAACTTCACTGCAATCGGCGGCAACCTGGCGGGGGCACCGG
  CCCTGATGGGCAACGTGGTCCTATGGAAGCCCAGTGACACTGCCATGCTGGCCAGCTATGCTGTCTACCG
  CATCCTTCGGGAGGCTGGCCTGCCCCCCAACATCATCCAGTTTGTGCCAGCTGATGGGCCCCTATTTGGG
  GACACTGTCACCAGCTCAGAGCACCTCTGTGGCATCAACTTCACAGGCAGTGTGCCCACCTTCAAACACC
  TGTGGAAGCAGGTGGCCCAGAACCTGGACCGGTTCCACACCTTCCCACGCCTGGCTGGAGAGTGCGGCGG
  AAAGAACTTCCACTTCGTGCACCGCTCGGCCGACGTGGAGAGCGTGGTGAGCGGGACCCTCCGCTCAGCC
  TTCGAGTACGGTGGCCAGAAGTGTTCCGCGTGCTCGCGTCTCTACGTGCCGCACTCGCTGTGGCCGCAGA
  TCAAAGGGCGGCTGCTGGAGGAGCACAGTCGGATCAAAGTGGGCGACCCTGCAGAGGATTTTGGGACCTT
  CTTCTCTGCAGTGATTGATGCCAAGTCCTTTGCCCGTATCAAGAAGTGGCTGGAGCACGCACGCTCCTCA
  CCCAGCCTCACCATCCTGGCCGGGGGCAAGTGTGATGACTCCGTGGGCTACTTTGTGGAGCCCTGCATCG
  TGGAGAGCAAGGACCCTCAGGAGCCCATCATGAAGGAGGAGATCTTCGGGCCTGTACTGTCTGTGTACGT
  CTACCCGGATGACAAGTACAAGGAGACGCTGCAGCTGGTTGACAGCACCACCAGCTATGGCCTCACGGGG
  GCAGTGTTCTCCCAGGATAAGGACGTCGTGCAGGAGGCCACAAAGGTGCTGAGGAATGCTGCCGGCAACT
  TCTACATCAACGACAAGTCCACTGGCTCGATAGTGGGCCAGCAGCCCTTTGGGGGGGCCCGAGCCTCTGG
  AACCAATGACAAGCCAGGGGGCCCACACTACATCCTGCGCTGGACGTCGCCGCAGGTCATCAAGGAGACA
  CATAAGCCCCTGGGGGACTGGAGCTACGCGTACATGCAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC228933 representing NM_001161504
  Red=Cloning site Green=Tags(s)
  
  MEAIPCVVGDEEVWTSDVQYQVSPFNHGHKVAKFCYADKSLLNKAIEAALAARKEWDLKPIADRAQIFLK
  AADMLSGPRRAEILAKTMVGQGKTVIQAEIDAAAELIDFFRFNAKYAVELEGQQPISVPPSTNSTVYRGL
  EGFVAAISPFNFTAIGGNLAGAPALMGNVVLWKPSDTAMLASYAVYRILREAGLPPNIIQFVPADGPLFG
  DTVTSSEHLCGINFTGSVPTFKHLWKQVAQNLDRFHTFPRLAGECGGKNFHFVHRSADVESVVSGTLRSA
  FEYGGQKCSACSRLYVPHSLWPQIKGRLLEEHSRIKVGDPAEDFGTFFSAVIDAKSFARIKKWLEHARSS
  PSLTILAGGKCDDSVGYFVEPCIVESKDPQEPIMKEEIFGPVLSVYVYPDDKYKETLQLVDSTTSYGLTG
  AVFSQDKDVVQEATKVLRNAAGNFYINDKSTGSIVGQQPFGGARASGTNDKPGGPHYILRWTSPQVIKET
  HKPLGDWSYAYMQ
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001161504
• ORF Size: 1509 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001161504.1, NP_001154976.1
• RefSeq Size: 3259 bp
• RefSeq ORF: 1512 bp
• Locus ID: 8659
• UniProt ID: P30038
• Cytogenetics: 1p36.13
• Protein Families: Druggable Genome
• Protein Pathways: Alanine, aspartate and glutamate metabolism, Arginine and proline metabolism, Metabolic pathways
• MW: 55.1 kDa
• Gene Summary: This protein belongs to the aldehyde dehydrogenase family of proteins. This enzyme is a mitochondrial matrix NAD-dependent dehydrogenase which catalyzes the second step of the proline degradation pathway, converting pyrroline-5-carboxylate to glutamate. Deficiency of this enzyme is associated with type II hyperprolinemia, an autosomal recessive disorder characterized by accumulation of delta-1-pyrroline-5-carboxylate (P5C) and proline. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jun 2009]