SH2D2A (NM_001161444) Human Tagged ORF Clone

CAT#: RC228868

SH2D2A (Myc-DDK-tagged)-Human SH2 domain containing 2A (SH2D2A), transcript variant 5

Product Images

Circular map for RC228868

• 

  Western blot validation of overexpression lysate (Cat# LY431896) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC228868 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SH2D2A
• Synonyms: F2771; SCAP; TSAD; VRAP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC228868 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTTCCCCCTGGCCCAGATATGTCCCCAAGGGAGTCACGAAGCCCCCATCCCAACCTTCAGCACCT
  TCCAGATCACAGACATGACCCGCAGGAGCTGCCAGAACCTGGGCTACACTGCGGCATCTCCCCAGGCCCC
  GGAGGCTGCCTCCAGCACAGGGAATGCTGAGAGGGCAGAGGAGGTGCCTGGAGAAGGAAGCCTGTTCCTG
  CAGGCCGAGACCCGGGCTTGGTTCCAGAAGACCCAGGCCCACTGGCTCCTGCAGCACGGGGCAGCCCCTG
  CCTGGTTCCATGGCTTCATCACCCGGAGGGAGGCAGAGAGGCTGCTGGAGCCCAAGCCTCAGGGGTGCTA
  CTTGGTGCGGTTCAGCGAGAGCGCGGTGACCTTCGTGCTGACTTACAGGAGCCGGACTTGCTGCCGCCAC
  TTCCTGCTGGCCCAGCTCAGGGACGGGCGCCACGTGGTGCTGGGCGAGGACAGCGCCCACGCGCGGCTGC
  AGGACCTGCTGCTGCACTACACCGCGCACCCGCTCAGCCCCTACGGGGAGACGCTCACCGAGCCCCTCGC
  CCGACAGACTCCTGAGCCTGCAGGACTTTCCCTGAGGACCGAAGAATCAAACTTTGGAAGCAAAAGCCAG
  GACCCAAACCCCCAGTACAGCCCAATCATCAAACAGGGGCAAGCCCCAGTCCCGATGCAGAAAGAGGGGG
  CCGGGGAGAAGGAGCCCTCCCAGCTGCTCAGGCCCAAGCCTCCCATCCCCGCCAAACCTCAGCTGCCCCC
  AGAAGTCTACACAATCCCTGTTCCACGACACCGCCCGGCCCCACGCCCCAAGCCCTCCAATCCTATCTAC
  AATGAGCCTGATGAACCCATAGCTTTCTATGCCATGGGCCGGGGCAGCCCTGGGGAAGCCCCCAGCAACA
  TCTATGTGGAAGTGGAAGATGAGGGCCTACCCGCCACCCTTGGGCACCCTGTCCTACGGAAGAGCTGGTC
  CAGGCCTGTCCCAGGAGGCCAGAATACAGGTGGCTCCCAGCTGCATTCTGAGAACTCTGTGATTGGGCAA
  GGCCCTCCCCTGCCCCACCAGCCCCCACCCGCCTGGAGACACACCCTCCCCCACAATCTTTCTAGACAGG
  TGCTTCAGGACAGAGGACAGGCATGGCTTCCCCTTGGGCCTCCTCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC228868 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEFPLAQICPQGSHEAPIPTFSTFQITDMTRRSCQNLGYTAASPQAPEAASSTGNAERAEEVPGEGSLFL
  QAETRAWFQKTQAHWLLQHGAAPAWFHGFITRREAERLLEPKPQGCYLVRFSESAVTFVLTYRSRTCCRH
  FLLAQLRDGRHVVLGEDSAHARLQDLLLHYTAHPLSPYGETLTEPLARQTPEPAGLSLRTEESNFGSKSQ
  DPNPQYSPIIKQGQAPVPMQKEGAGEKEPSQLLRPKPPIPAKPQLPPEVYTIPVPRHRPAPRPKPSNPIY
  NEPDEPIAFYAMGRGSPGEAPSNIYVEVEDEGLPATLGHPVLRKSWSRPVPGGQNTGGSQLHSENSVIGQ
  GPPLPHQPPPAWRHTLPHNLSRQVLQDRGQAWLPLGPPQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001161444
• ORF Size: 1167 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001161444.1, NP_001154916.1
• RefSeq Size: 1445 bp
• RefSeq ORF: 1170 bp
• Locus ID: 9047
• UniProt ID: Q9NP31
• Cytogenetics: 1q23.1
• Protein Pathways: VEGF signaling pathway
• MW: 42.9 kDa
• Gene Summary: This gene encodes an adaptor protein thought to function in T-cell signal transduction. A related protein in mouse is responsible for the activation of lymphocyte-specific protein-tyrosine kinase and functions in downstream signaling. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]